LOCUS       sequence-327052-        6346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline resistance reporter of ChnR activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Thompson MG, Sedaghatian N, Barajas JF, Wehrs M, Bailey CB, Kaplan 
            N, Hillson NJ, Mukhopadhyay A, Keasling JD
  TITLE     Isolation and characterization of novel mutations in the pSC101 
            origin that increase copy number.
  JOURNAL   Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.1038/s41598-018-20016-w.
  PUBMED    29371642
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-01-25"; volume: "8(1):1590. doi"; pages: " 
            10.1038/s41598-018-20016-w"
COMMENT     Sequence Label: pMGT1
FEATURES             Location/Qualifiers
     source          1..6346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1467..2417)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     promoter        complement(4107..4184)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4390..4409
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             5002..6201
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatt gtctttcttc agctcgctga tgatatgcgt acgggttttg
      961 ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc agatccggct
     1021 tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat tttttcccca
     1081 cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc agcatcgcct
     1141 gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg tgttcaattt
     1201 catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt acatgctgtt
     1261 catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca aaaactcgta
     1321 aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg gacagttttc
     1381 cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt gatgcttcac
     1441 tgatagatac aagagccata agaacctcag atccttccgt atttagccag tatgttctct
     1501 agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc atacttactt
     1561 tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc agttaaagca
     1621 tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat ggttgttggt
     1681 attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag atccatttgt
     1741 ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc
     1801 aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac ccattggtta
     1861 agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa ttcatcaagg
     1921 ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa taaccactca
     1981 taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt atattttatg
     2041 aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa ttctaatttt
     2101 tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt aaccaaagga
     2161 ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa tacaccataa
     2221 gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa cgataccgtc
     2281 cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca gcataaaatt
     2341 agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt tgctttgaaa
     2401 acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact ataccaattg
     2461 agatgggcta gtcaatgata attactagtc cttttcccgg gtgatctggg tatctgtaaa
     2521 ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt aaattccgct
     2581 agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag aataaagaaa
     2641 gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact actttagtca
     2701 gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca aaacagacct
     2761 taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct gaatattcct
     2821 tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc
     2881 gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca
     2941 tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat
     3001 ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga
     3061 ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac
     3121 ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta gtcaatagag
     3181 gagactgaat tttcagacac gagattggtg tgttcctgtc tgatttcgat gggtgttttc
     3241 ccaaaaaatt tttgaaatac tcgggtcata tggctctgat cgcaaaaacc ggcttcactg
     3301 gcaatcactt tcagtggaat ttcttttttt gaagtgagca aacgtttagc aaattgcacc
     3361 ctgagattca tgatgtaggc agcaggggaa ttaccaaaat cgactttaaa ttttctcatt
     3421 aaatgaggca cgctcatctt gactaatccc gctaaatctt cgagggtaat tttaatgctc
     3481 atgttttcat tgataaattc taataacagt ctgcgttgta ggggagtaaa accagaacgg
     3541 cactgtcctt ccttaaaatc taatttggca tactgacgga gtaaatgcag ggcgatctgg
     3601 tttttaatgc tctctaaata caggtttcca cctaaaccac cttgctttaa ttctagttct
     3661 aaaagtgaag tcagagcagg taaaacatga tcttccacac cgacttcgtc tctgattcgg
     3721 atcgaagcga tatcataatc aaacacctta ttcgccattg aggtgatgga gtcatgcgaa
     3781 atgtagacat gggtgacggc aatatggtca ttccacgccc agcgggaatc ttcaccacag
     3841 gtcaacaagg agacataacc cggcttaacc acttgggtat cccaagaggc atcgcctttt
     3901 ctacgcattt ccgccgcact ttttttatag ttgacgatca tgtagtcacg catggcagga
     3961 atatgcacat cgaggttggt atattcgtac cctctgattg caatgttttc ccaaccggca
     4021 ttcatgctgt caattgtgac attacccgga atccataaca cgacatcttc gggtttgatc
     4081 agcgtatttg ctttgtctgt gctcatattc accaccctga attgactctc ttccgggcgc
     4141 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     4201 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     4261 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     4321 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     4381 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     4441 gccggccacg atgcgtccgg cgtagaggat cgagtggccg cgggatatca ctagtgcggc
     4501 cggtggtggt ggtccaaact atccgaatat gtaagtctaa tcacttcaag gaagaggttt
     4561 cccatttccc ttctttctag cagatgaaga agcttgcaac taaaagagat tgtttggatc
     4621 agttacccaa aatcgttgaa aagattttaa ctcttcgatt tttatttttt aggtaatcct
     4681 agccctctcg ggggctagga ttaaaaattt taagttattc caacacgaat gacaaattgt
     4741 tcaatgcaaa ataaaaacat acaatatata aatatatttt ttaattaaaa cataagatta
     4801 caataaaata agaattttta tttggagttt gttttttttc tacaatgatc attatgtaca
     4861 atttttaggt tcaccccatc caagccttgt gattgcattc ctgcgattct ttattcaatg
     4921 aataagcaat gctattaatc agcaatgaat aaccagcact gcagattttg aataaattca
     4981 catgtcgtaa tggagggtac catgaaaccc aacatacccc tgatcgtaat tctgagcact
     5041 gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc
     5101 gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg
     5161 ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg
     5221 ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact acgccatcat ggcgacagcg
     5281 cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg
     5341 gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc
     5401 ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg
     5461 atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat
     5521 ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc
     5581 cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc
     5641 gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg
     5701 gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc
     5761 gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg
     5821 ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg
     5881 cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt
     5941 ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag
     6001 gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc
     6061 ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca
     6121 ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca
     6181 gggcaacgag ccgatcgcta accgttgatc gtgctatgat cgactgatgt catcagcggt
     6241 ggagtgcaat gtcatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     6301 taacggcgca gtggcggttt tcatggcttg ttatgactgt tttttt
//
