LOCUS       sequence-327057-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be
            co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain 
            DiB-RM‐split-Zip protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov
            MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
  TITLE     Computational redesign of a fluorogen activating protein with 
            Rosetta.
  JOURNAL   PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
  PUBMED    34748541
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-11-8"; volume: "17(11):e1009555. doi"; pages: " 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov"
COMMENT     Sequence Label: pET11a-Z-N-DiB-RM
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             430..441
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(519..537)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      533..580
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(755..760,761..777,778..783))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(810..828)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgggtc atcaccacca ccatcacctc
       61 gaatcaacca gcttgtacaa gaaatccagc tccacgccgc cgcgtggcgt gaccgtagta
      121 aataatttcg actgcaaacg ttatctcggt acctggtatg agattgcccg ttttgatcac
      181 cgcgcagaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga tgacggcggc
      241 ctgaatgtca tcttcaaagg ctataaccct gacagaggaa tgtggcagca gtacgttggg
      301 aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtggcgtt ctttggtggc
      361 tctggctctg gctcgagcgc cctcaaaaaa gaattgcagg caaacaaaaa agaacttgcg
      421 cagctgaagt gggagttaca agctctgaaa aaggaactgg cgcagtaagg atccggctgc
      481 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      541 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      601 cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
      661 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
      721 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
      781 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tatagg
//
