LOCUS       sequence-327061-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of MERS-CoV soluble spike trimer protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R619-M88-303: CMV51p> MERS-CoV
            S-2P-T4f-3C-His...
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3477..3488
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6799..6822
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6826..6849
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6856..6879
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6919..6942
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6944..6968)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7030..7048)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7031..7048)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7068..7085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7074..7298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7344..7772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7431..7450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7641..7662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7781..7801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7786..8115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7966..8101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8028..8047
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8182..8976
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8236..8255)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8846..8865
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9150..9271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9187..9206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9241..9260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9320..9336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9320..9336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9333..9355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9344..9360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9368..9374,9375..9392,9393..9398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9413..9434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9551..9568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9722..10310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9802..9821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10481..11272,11273..11341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11104..11123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11342..11446)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgattcacag cgtgttcctc ctgatgttcc ttctgacccc caccgagagc
     2881 tacgtggacg tcgggccaga tagcgtgaag tcggcgtgta tcgaggtgga catccagcag
     2941 accttcttcg ataagacctg gcctaggccc atcgacgtgt ccaaggcgga tggcatcatc
     3001 tacccgcagg gtcggactta ctccaacatc actattacct accaaggcct gttcccatat
     3061 caaggagatc acggcgatat gtacgtctac tcggctggac atgcgaccgg cacaaccccc
     3121 cagaaactgt ttgtggccaa ctactcccag gacgtgaagc agttcgccaa tggtttcgtc
     3181 gtgcggattg gagcggccgc caactcgacc ggaaccgtga ttattagccc tagcacctcc
     3241 gccaccatca ggaagatcta cccggccttt atgcttgggt cgagcgtggg aaacttcagc
     3301 gacggcaaaa tgggccgctt cttcaaccat accctcgtgc tgctgcctga cggctgcggt
     3361 accctcctcc gcgccttcta ctgtatcctg gagcctagat ccggcaacca ttgcccggct
     3421 gggaactcat acacctcctt cgcaacttac cacactccag ccaccgattg ctcagatggg
     3481 aactacaacc ggaatgcgtc gctgaactcg ttcaaggaat acttcaactt gcgaaactgc
     3541 actttcatgt acacctacaa catcactgag gacgaaattc tcgagtggtt tggtattact
     3601 cagaccgccc agggagtgca tctgttctcc tcgcgctatg tcgacctata cggtggaaac
     3661 atgttccagt tcgccacact tcctgtgtat gacaccatta agtactattc aatcattcct
     3721 cactcgatta gatctatcca gtccgacaga aaggcctggg ccgcattcta cgtgtacaag
     3781 ctgcagcctc tgaccttcct gctggatttt agcgtggacg gctacatcag acgcgccatt
     3841 gactgcggct tcaatgacct gagccagctg cactgctcgt acgagtcttt cgatgtggag
     3901 tcaggcgtat actcagtttc gtctttcgaa gcgaagccga gcggttccgt cgtcgaacaa
     3961 gccgaagggg tggaatgcga cttcagcccc ttgttgagcg ggactccccc acaagtgtac
     4021 aacttcaagc gcctggtgtt cactaactgc aactataacc tgactaagct gctttcgctg
     4081 ttctccgtga acgactttac ctgttcccaa atctcccctg ccgctatcgc ctccaactgt
     4141 tactcaagcc tcatcctgga ttacttctcc tacccactgt cgatgaagag tgacctctcg
     4201 gtgtcctctg ccggaccgat cagccagttc aattacaagc agagctttag caaccccact
     4261 tgcctcattc tcgcaaccgt gccccacaac ctgaccacga ttactaagcc cctgaagtac
     4321 tcatatatca acaagtgctc ccgcttcctc tccgacgacc gcactgaagt cccgcagctc
     4381 gtcaacgcaa accagtactc cccctgcgtg tccatcgtgc cctccaccgt gtgggaagat
     4441 ggggattact accggaagca gctgtcaccg ctcgaaggcg gaggatggct tgtggcctcc
     4501 ggaagcaccg tggccatgac cgaacagctg cagatggggt tcggcatcac tgtgcagtac
     4561 ggaacggaca ccaactccgt atgccccaag ctggagttcg ccaacgacac gaagattgct
     4621 agccagctcg gcaactgcgt ggagtactca ctgtacgggg tgtcaggacg cggcgtgttc
     4681 cagaactgca ccgccgtggg agtcagacag cagcgcttcg tgtacgacgc ctaccaaaac
     4741 ctggtcggat actactctga cgacgggaac tactactgcc tgcgggcttg cgtgtccgtg
     4801 ccggtgtccg tgatctacga caaggagaca aagacccacg ccaccctctt tggatcagtg
     4861 gcctgcgagc acatctcgtc aactatgtcg caatactcgc ggtccacacg gtccatgctg
     4921 aagcgccgcg attcgaccta tggaccgctg cagactcctg tgggctgtgt gcttggactc
     4981 gtgaacagct ccctgttcgt ggaggattgc aagctgccgc ttggacaaag tctgtgcgcg
     5041 ctgccagata ctccgtccac tctgacccct gcttccgtcg gatccgtgcc cggagaaatg
     5101 cggctggcgt ccattgcctt caaccacccg atccaagtgg accagctgaa ctcctcatac
     5161 tttaagttga gcatccctac gaatttctcg ttcggcgtga cccaggagta catccagact
     5221 actattcaaa aggtcaccgt ggactgcaag cagtatgtct gcaacggatt ccagaagtgc
     5281 gagcagctgc tgagagaata tgggcagttt tgctccaaaa tcaaccaggc cctccatgga
     5341 gccaacttgc ggcaggacga ttccgtcagg aacctgttcg cgtccgtgaa gtcctcccag
     5401 tcgagcccaa tcatcccggg attcggcgga gatttcaacc tgacgcttct ggaaccggtg
     5461 tccatttcca ccgggagcag atcggccaga agcgccattg aagatctgct gttcgataag
     5521 gtcaccatag cagaccctgg ctacatgcag ggatacgacg actgcatgca gcagggtccg
     5581 gctagcgcaa gagatctgat ctgcgcgcaa tacgtggcgg gctacaaggt cctgccgcct
     5641 ctcatggacg tgaatatgga ggcggcttac acctcctctc tgctcgggtc catcgcggga
     5701 gtgggatgga ctgctggcct ttcgtcgttc gccgccattc cgttcgccca gtccattttc
     5761 taccggctga acggcgtcgg aatcactcaa caagtcctct ccgaaaacca gaagctgatt
     5821 gccaacaagt tcaaccaggc actgggagcc atgcagaccg ggttcaccac cacgaacgaa
     5881 gcgttccaca aggtccagga tgccgtcaac aacaacgccc aagccctctc caaactcgcc
     5941 tcagagcttt ccaatacctt cggcgccatt tccgcctcca tcggcgacat tatccagagg
     6001 ttggaccctc ctgaacaaga tgcgcagatc gaccgcctca tcaacggcag gcttaccacc
     6061 ctgaacgcct tcgtggctca gcagctcgtg cggagcgaga gtgctgccct gtcggctcag
     6121 ttggcaaagg acaaagtgaa cgaatgcgtg aaggcccagt caaagcggtc gggcttctgc
     6181 ggtcaaggaa cccacattgt gtccttcgtc gtgaacgccc cgaatggcct gtacttcatg
     6241 catgtcggct actacccctc caaccacatc gaagtcgtgt ccgcgtacgg actctgcgat
     6301 gccgcgaacc ccaccaactg tattgccccc gtgaacggct actttattaa gaccaacaac
     6361 actcggatcg tggatgagtg gtcgtacacc gggagctcct tctacgctcc ggaacctatc
     6421 acgtcactga acaccaaata cgtcgcgccg caagtcacct atcagaacat ctcgactaac
     6481 ttgcctcccc ctctgctggg aaacagcacc ggcattgact ttcaagacga gctggacgaa
     6541 ttcttcaaga acgtcagcac ctccataccc aacttcggat cccttaccca aatcaatacc
     6601 accttgctcg acctcaccta cgagatgctg tcactgcaac aggtcgtcaa ggccctgaac
     6661 gaaagttaca ttgacctcaa ggagctgggg aactacacct acggaagcgg atacatccct
     6721 gaggcaccaa gggacgggca ggcatacgtc cggaaggacg gagaatgggt gctgctgtcc
     6781 accttcctgg gacggagcct ggaagtgctg ttccaaggcc cgggacatca ccaccatcac
     6841 catcatcact cagcatggtc gcacccccaa tttgagaagg gcggtggttc cggtggagga
     6901 ggatcgggag gttccgcatg gtcccatccc caattcgaga aataatcaac tttcttgtac
     6961 aaagttgtga aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     7021 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     7081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7201 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7261 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7321 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7501 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7561 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7621 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7681 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7741 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7801 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7861 tcagcaacca ggtgtggaaa gtccctaggc tccccagcag gcagaagtat gcaaagcatg
     7921 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8041 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8101 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     8161 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8221 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8281 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8341 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     8401 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8461 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8521 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8581 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8641 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8701 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8761 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8821 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8881 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8941 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9001 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     9061 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9121 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9301 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11461 aagtgccacc tgacgtc
//
