LOCUS       sequence-327063-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the DiB-RM‐split protein. This plasmid needs to be 
            co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain 
            DiB-RM‐split protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov
            MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
  TITLE     Computational redesign of a fluorogen activating protein with 
            Rosetta.
  JOURNAL   PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
  PUBMED    34748541
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-11-8"; volume: "17(11):e1009555. doi"; pages: " 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov"
COMMENT     Sequence Label: pET11a-N-DiB-RM
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(409..427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      423..470
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(645..650,651..667,668..673))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(700..718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        786..890
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgggt catcaccacc accatcacct
       61 cgaatcaacc agcttgtaca agaaatccag ctccacgccg ccgcgtggcg tgaccgtagt
      121 aaataatttc gactgcaaac gttatctcgg tacctggtat gagattgccc gttttgatca
      181 ccgcgcagaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg
      241 cctgaatgtc atcttcaaag gctataaccc tgacagagga atgtggcagc agtacgttgg
      301 gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtggcgt tcttttaagg
      361 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      421 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      481 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
      541 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
      601 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
      661 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      901 aacatttcc
//
