LOCUS       sequence-327071-       13526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged FL mouse talin1 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 13526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pCDNA_GFP_mTLN1-FL-LBD
FEATURES             Location/Qualifiers
     source          1..13526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             771..800
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(801..803,804..806,807..1517)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(801..803,804..806,807..1517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(846..867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1107..1126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1524..1547
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9215..9244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9260..9277
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9300..9317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9306..9530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9576..10004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9663..9682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9873..9894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10013..10033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10018..10347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10198..10333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10260..10279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10395..10442
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10461..10835
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAGWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10965..11086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11002..11021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11056..11075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11135..11151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11135..11151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11148..11170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11159..11175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11183..11189,11190..11207,11208..11213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11228..11249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11366..11383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11537..12125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11617..11636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12296..13087,13088..13156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12919..12938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13157..13261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13336..13355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg ctagcggtac cgccaccatg catcaccacc
      781 accatcatca tcaccaccat atggtgagca agggcgagga gctgttcacc ggggtggtgc
      841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      901 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      961 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1021 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1081 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1201 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1261 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc accctcggca
     1501 tggacgagct gtacaaggga tccctggaag ttctgttcca ggggccccat atggttgcgc
     1561 tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag ccatctacca
     1621 tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg gctggccctc
     1681 ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc tggctagagg
     1741 ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag tacagaaaaa
     1801 agcagagacc cctgaagatc cggatgttag acggaacggt gaagactatc atggtggatg
     1861 actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt atcaccaacc
     1921 atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag gggacaggga
     1981 ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag ctaaagcaga
     2041 agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg agggaacagg
     2101 gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca gaccagaatg
     2161 tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga gatgacatcc
     2221 tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc ttccagtgcc
     2281 agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt gacctgaagg
     2341 acttcctgcc caaggagtat gtgcagcaga agggagagcg taagatcttt caggcacaca
     2401 agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag ctggcccgtt
     2461 ccctcaagac ttacggtgtc tccttcttcc tagtccagga aaagatgaag gggaagaatc
     2521 aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg gatcagcaga
     2581 ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct gcctctccca
     2641 agagctttac tctggacttt ggagactatc aggatggcta ctactcagta cagacgacag
     2701 aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt aagaagaaaa
     2761 aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg gaggactcag
     2821 tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg aaagtggagc
     2881 acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct gagaatttcc
     2941 aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg caccgaggac
     3001 acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac tccagcatgc
     3061 aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc cctcttggcc
     3121 aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag catgagatcc
     3181 actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg acagcaggag
     3241 atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc tcttccaacc
     3301 tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat gaaggcggca
     3361 atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg gaactgcttc
     3421 gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgctggca gccgggaacg
     3481 tgggccaggc cagtggggag ctgttgcagc aaattgggga aagtgacact gacccccact
     3541 tccaggatgt tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc gccctggtcc
     3601 tcaaggccaa gagtgtggcc cagcgcacag aggattccgg gcttcagacc caagtgattg
     3661 ctgcagctac gcaatgtgct ctgtccactt cccagctggt ggcctgtacc aaggtggtag
     3721 cacctacaat cagctcacct gtgtgccaag aacagctagt cgaggctggg cgactggtgg
     3781 ccaaagctgt ggagggctgt gtgtctgcct cgcaagcagc tacagaagat ggacagctgc
     3841 ttcgaggggt aggagcagca gccacggctg tcacccaggc cctcaatgag ctgctgcagc
     3901 acgtgaaggc ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa gccactgata
     3961 ccatcctcac cgtcactgaa aacatcttca gctccatggg tgatgctggg gagatggtgc
     4021 gccaggcccg catcctggcc caagccacct cagacctggt caatgctatc aaggccgatg
     4081 ctgaggggga gagtgatctg gagaactcta gaaagctcct gagtgctgcc aagatcctcg
     4141 ctgatgccac cgccaagatg gtggaggcgg ccaagggagc agccgcccac cctgacagtg
     4201 aggaacagca gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc accaatgcag
     4261 ctgcgcagaa cgccatcaag aagaagttgg tgcagcgcct ggagcatgca gccaagcaag
     4321 ctgcagcctc tgcaacacag accattgctg cagcccaaca tgcagcctct gcccccaagg
     4381 cctctgcggg cccccagccc ctactggtgc agagctgtaa ggccgtggca gagcagattc
     4441 cgttgctggt gcagggtgtc cgaggaagtc aagctcagcc tgacagccct agtgctcagc
     4501 tcgccctcat tgctgccagc cagagcttcc tgcagccagg tgggaagatg gtggcagcgg
     4561 caaaagcctc agtgccaaca attcaggacc aggcttcagc gatgcagctg agtcagtgcg
     4621 caaagaacct aggcactgcc ctggccgagc tgcgcactgc tgctcagaag gctcaggagg
     4681 catgtggacc tttggagatg gattctgcac tgagtgttgt acaaaatcta gagaaagacc
     4741 tacaggaaat aaaagcagca gctcgagatg gcaagcttaa gcccttacct ggggaaacaa
     4801 tggagaagtg tacccaagat cttggcaaca gcaccaaagc ggtgagctct gccatcgcca
     4861 agctgctggg ggagattgcc cagggcaatg agaactatgc aggtattgca gctcgggatg
     4921 tagctggtgg actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg ctgacatcag
     4981 atcctgcagt gcaggccatt gtgcttgaca cagccagcga cgttctggac aaggccagca
     5041 gcctcattga agaggcaaaa aaagcatctg gacacccagg ggacccagaa agccagcaaa
     5101 ggcttgctca ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc agctgcctgc
     5161 ctggccaaag agacgtggat aatgccctac gagccgttgg agatgccagc aagcgactcc
     5221 tgagtgactt gcttcctcca agcacgggga catttcaaga agcacagagc cgattgaatg
     5281 aagctgcagc tgggttaaat caggccgcca cagaactggt gcaagcctcc cgaggaactc
     5341 ctcaggacct ggctcgggcc tccggccgat ttggacagga cttcagcacc ttcctggaag
     5401 ctggtgtgga gatggccgga caggccccga gccaggagga ccgagcccag gtggtgtcca
     5461 acctgaaggg catatccatg tcttcaagca aacttctcct tgccgctaag gccttgtcca
     5521 cagaccctgc ttctcccaac ctcaagagtc agctggctgc ggctgcccgg gcagtgacgg
     5581 acagcatcaa ccagctcatc accatgtgca cccagcaggc acctggccag aaggagtgtg
     5641 acaatgcact tcggcagcta gagacagtcc gagaactcct ggagaatcca gtccagccca
     5701 tcaacgacat gtcctacttc ggttgcttgg acagtgtcat ggagaactcc aaggtcctag
     5761 gtgaggccat gactggcatc tcccaaaatg ccaagaatgg aaatctgccg gagtttggag
     5821 atgccattgc cacagcctcc aaggctctct gtggcttcac ggaggcagcc gcacaggcag
     5881 catatctagt tggtgtctct gaccccaaca gccaagctgg acagcaagga ctggtggaac
     5941 ccacacagtt tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt ctgggggagc
     6001 ctggctgtac ccaggcccag gtgttatctg cagccactat tgtagccaaa cacacatctg
     6061 cattgtgtaa cagctgtcgc ctggcttccg ctaggactgc caatcccact gccaagcgcc
     6121 agtttgtaca gtcagccaag gaggtggcca acagtacagc caatcttgtc aagaccatca
     6181 aggcactaga tggggacttc acagaagaga accgtgccca gtgccgagca gccacagccc
     6241 ctctgctgga agctgtggat aacctgagtg cctttgcctc caaccctgag ttctccagcg
     6301 tccctgccca gatcagccct gagggcagag cagccatgga gcccattgta atctctgcta
     6361 agacaatgtt ggagagtgct ggagggctca tccagacagc tcgggcgtta gcagtcaatc
     6421 cccgagaccc cccacgttgg tctgtgctag ctggccactc tcggactgtc tcagattcca
     6481 tcaagaaact tattacaagc atgagggaca aagccccagg gcagctggag tgtgagacag
     6541 ccattgcggc tctgaacagc tgcttgcggg acctagatca ggcttcgctt gccgctgtca
     6601 gccagcagct tgctccccgt gaaggaatct ctcaagaggc tttgcacacc cagatgctca
     6661 ctgcagtgca agaaatttct catctcattg agccgctggc cagcgctgct cgggctgaag
     6721 cctcccagct gggacacaag gtgtcccaaa tggcccagta ctttgaacca ctcaccctgg
     6781 ctgcagtggg tgctgcgtct aagaccctga gccacccgca gcagatggca cttctggacc
     6841 agactaaaac gttggcagag tctgccttgc agttgctata tactgccaag gaggctggtg
     6901 gtaaccccaa gcaagcagca cacacccagg aagccctgga ggaggctgtg cagatgatga
     6961 cagaggccgt agaagacctg acgacgaccc tcaacgaagc agccagtgct gcaggagtcg
     7021 ttggcggcat ggtggactct atcactcagg ccatcaacca gctagatgaa ggacctatgg
     7081 gtgacccaga aggctcattc gtagattacc agacaaccat ggtgaggaca gccaaggcca
     7141 ttgctgtcac tgttcaggag atggtaacca agtcaaacac cagccctgaa gagctaggcc
     7201 ctcttgccaa ccagctgacc agtgactatg gccgactggc ctcacaagcc aagcctgcag
     7261 ctgtggctgc tgaaaatgaa gagataggcg ctcacatcaa acaccgagta caggagctgg
     7321 gccatggctg ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc cccagtgatg
     7381 tctacaccaa gaaggagctc atagagtgtg cccgcagagt gtcagagaag gtctcccatg
     7441 ttctggctgc actccaggct gggaatcgtg gtacccaggc ctgcattaca gcagccagtg
     7501 ctgtgtctgg tatcattgct gacctcgaca ccaccatcat gttcgctact gctggcacac
     7561 ttaaccgtga gggtgctgaa acttttgctg accaccggga gggtatctta aagacagcaa
     7621 aggttcttgt ggaggacacc aaggtcctag tgcagaatgc agctgggagc caggagaagt
     7681 tggcacaagc cgcccagtcc tccgtggcca ccattacccg cctcgctgat gtggtcaagc
     7741 tcggtgcagc cagcctagga gccgaagacc ctgaaactca ggtggtgctg atcaatgcag
     7801 taaaggacgt agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct gcagcgggca
     7861 aagttgggga tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg atggtgacca
     7921 atgtgacatc attgctcaag acagtgaagg ctgtggaaga tgaggccacc aaaggcacac
     7981 gggccctaga ggcaaccaca gagcacatac gtcaggaact ggcggtcttc tgttccccag
     8041 agccacctgc caagacctct acccctgaag acttcatccg aatgaccaag ggtattacta
     8101 tggcaacagc caaagccgtt gctgctggca attcctgtcg acaggaagat gtcattgcca
     8161 cagccaatct gagccgacgg gctattgcgg acatgcttcg ggcttgcaag gaagcagctt
     8221 tccacccaga agtggcgcct gatgtacggc tccgagccct gcactatggc cgggagtgtg
     8281 ccaatggtta cctggaactg ctggaccacg tgctgctgac ccttcagaag ccaaacccag
     8341 acctgaagca gcagctaact ggacactcaa agcgggttgc tggctctgtg actgagctca
     8401 tccaggctgc tgaagccatg aaaggaacag agtgggtgga cccagaggac cctactgtca
     8461 ttgctgagaa tgagctcttg ggagccgcag ccgccatcga ggctgcagcc aagaagctgg
     8521 agcagctgaa gcccagggcc aaacccaagg aggcggatga gtccttgaac tttgaggaac
     8581 aaatcctaga agctgtcaag tccatcgctg cagccaccag tgcactggta aaggctgcgt
     8641 cagcagccca gagggagctg gtggctcaag gaaaggtggg cgccattcca gccaatgcac
     8701 tggatgatgg gcagtggtcg cagggcctca tttccgctgc ccgtatggtg gctgcggcca
     8761 ccaacaatct gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc caggagaaac
     8821 tcatttcctc agccaagcaa gtagctgcct ccacagctca gctcctggta gcttgcaagg
     8881 tcaaagcaga tcaggactct gaggcaatga aacggctcca ggctgctggc aatgcagtga
     8941 agagggcctc agataacctg gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg
     9001 agaatgagac ggtggtggtg aaggagaaaa tggttggggg cattgcccag attatcgcag
     9061 cacaggaaga gatgcttcgg aaggaacgag agctggaaga ggctcggaaa aagctcgccc
     9121 agatccggca gcagcagtac aagttcttgc cttcagagct tcgagacgag cactgagaat
     9181 tcgcggccgc tcgagtctag agggcccgcg gttcgaacaa aaactcatct cagaagagga
     9241 tctgaatatg cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     9301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     9601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
    10021 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10081 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    10141 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    10201 ccgcccctaa ctccgcccag ttccgccctt tctccgcccc atggctgact aatttttttt
    10261 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
    10321 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
    10381 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
    10441 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
    10501 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
    10561 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
    10621 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
    10681 tgtacgccgg gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
    10741 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
    10801 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
    10861 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    10921 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    10981 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    11041 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    11101 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    11161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    11221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    11281 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11341 tgtgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    11761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11941 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    12001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    12241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    12301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    12361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    12421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    12481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    12781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    12841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    13021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    13081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    13141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    13261 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    13321 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    13381 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    13441 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
    13501 cgatgtacgg gccagatata cgcgtt
//
