LOCUS       sequence-327073-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: C1(1-29)-TurboID-V5_pLX304
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             513..908
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    968..1085
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1202..1505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1506..1709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1660..1680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1732..1756
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1933..2889
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2896..2937
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(2942..2966)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2999..3587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3052..3072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3470..3481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3659..3892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3970..4091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4007..4026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4061..4080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4131..4266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4286..4305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4287..4305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4315..4332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4315..4331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4324..4346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4473..4928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4560..4579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4770..4791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4954..5058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5059..5127,5128..5919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6090..6678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6579..6598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6832..6849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6966..6987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7002..7007,7008..7025,7026..7032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7040..7056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7045..7067
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7064..7080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7064..7080
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7099..7119
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7101..7119
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7147..7373
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7374..7554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7601..7726
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8219..8452
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8637..8681
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct caagaagaat
      541 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
      601 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
      661 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
      721 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
      781 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
      841 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
      901 gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg gcagtattca
      961 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1021 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1081 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc
     1141 ggggttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     1201 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     1261 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     1321 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     1381 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     1441 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     1501 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     1561 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     1621 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1681 tacggtggga ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg
     1741 tacaaaaaag caggcttcga aggagataga accatggacc ctgtggtcgt gctggggctg
     1801 tgcctgagct gtctgctgct gctgagcctg tggaagcagt cctacggagg cgggggatca
     1861 ggaagcggcg gccgcggatc aggaagcgga tcaggaagcg gatcaggaag cggatcagga
     1921 agcggatcag gaaaagacaa tactgtgcct ctgaagctga tcgctctcct ggctaatggc
     1981 gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc cgctatcaac
     2041 aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc
     2101 tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg acagctggac
     2161 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     2221 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     2281 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     2341 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     2401 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     2461 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct
     2521 gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg
     2581 gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg
     2641 gccgctacgc tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg
     2701 gctccatatc tgccacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg
     2761 atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg
     2821 ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct
     2881 gccgaaaagg ctagcggcaa gcccatcccc aaccccctgc tgggcctgga cagcacctaa
     2941 gacccagctt tcttgtacaa agtggtgcta gcgctaaccg gtggcgcgtt aagtcgacaa
     3001 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3061 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3121 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3181 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3241 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3301 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3361 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3421 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3481 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3541 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     3601 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3661 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     3721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     3901 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     3961 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4021 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4081 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4141 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4201 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4261 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4321 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4381 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4441 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4501 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4561 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4681 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4741 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4801 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4861 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4921 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5041 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5101 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5161 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5221 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5281 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5341 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5401 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5461 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5521 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5581 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5641 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5701 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5761 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5821 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5881 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6721 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6781 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6841 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6901 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6961 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7021 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7081 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7141 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7201 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7261 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7321 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7381 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7441 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7501 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7561 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7621 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7681 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7741 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7801 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7861 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7921 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7981 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8041 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8101 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8161 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8221 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8281 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8341 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8401 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8461 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8521 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8581 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8641 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8701 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8761 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8821 cgtttcagac ccacctccca accccgaggg gaccc
//
