LOCUS       sequence-327079-        8485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  insertin of CBP degrom.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Zhu Y, Denholtz M, Lu H, Murre C
  TITLE     Calcium signaling instructs NIPBL recruitment at active enhancers 
            and promoters via distinct mechanisms to reconstruct genome 
            compartmentalization.
  JOURNAL   Genes Dev. 2021 Jan 1;35(1-2):65-81. doi: 10.1101/gad.343475.120. 
            Epub 2020 Dec 17.
  PUBMED    33334824
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2021-01-1"; volume: "35(1-2):65-81. doi"; pages: " 
            10.1101/gad.343475.120. Epub 2020 Dec 17"
COMMENT     Sequence Label: px330 CBP gRNA
FEATURES             Location/Qualifiers
     source          1..8485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        97..345
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     97..117
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     268..287
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        367..442
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        539..824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        826..1080
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          1081..1308
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1320..1329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1329..1394
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1401..1421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1446..5546
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1446..5546
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5547..5594
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5622..5639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5628..5835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5844..5984
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5844..5973
                     /label=AAV2 ITR
     rep_origin      6059..6514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6356..6377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6531..6550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6650..6672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6710..6728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6796..6900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6901..6969,6970..7761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7119..7138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7932..8485,1..35)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8421..8440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
       61 ggttcctggc cttttgctgg ccttttgctc acatgtgagg gcctatttcc catgattcct
      121 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
      181 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
      241 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
      301 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgccat tggggatcag
      361 ctcatcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      421 aaaagtggca ccgagtcggt gcttttttgt tttagagcta gaaatagcaa gttaaaataa
      481 ggctagtccg tttttagcgc gtgcgccaat tctgcagaca aatggctcta gaggtacccg
      541 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      601 cgtcaatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      661 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      721 tcaatgacgg taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc
      781 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      841 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      901 ttttttaatt attttgtgca gcgatggggg cggggggggg cggggcgggg cgaggggcgg
      961 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     1021 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     1081 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1141 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1201 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1261 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1321 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
     1381 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
     1441 cagccgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
     1501 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
     1561 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
     1621 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
     1681 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     1741 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     1801 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     1861 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     1921 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     1981 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     2041 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     2101 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     2161 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     2221 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     2281 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     2341 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     2401 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     2461 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     2521 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     2581 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     2641 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     2701 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     2761 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     2821 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     2881 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     2941 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     3001 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     3061 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     3121 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     3181 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     3241 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     3301 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     3361 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     3421 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     3481 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     3541 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     3601 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     3661 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     3721 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     3781 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     3841 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     3901 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     3961 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     4021 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     4081 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     4141 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     4201 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     4261 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     4321 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     4381 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     4441 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     4501 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     4561 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     4621 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     4681 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     4741 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     4801 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     4861 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     4921 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     4981 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     5041 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     5101 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     5161 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     5221 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     5281 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     5341 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     5401 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     5461 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     5521 tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg
     5581 caaaaaagaa aaagtaagaa ttcctagagc tcgctgatca gcctcgactg tgccttctag
     5641 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5701 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5761 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag
     5821 caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     5881 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     5941 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     6001 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     6061 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6121 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6181 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6241 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     6301 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6361 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     6421 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6481 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     6541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     6601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     6721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7141 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7441 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
     7621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8461 cacctctgac ttgagcgtcg atttt
//
