LOCUS       sequence-327082-       10129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression GgPCFT protein with C terminally tagged GFP-His tag 
            cleavable with TEV protease..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Parker JL, Deme JC, Kuteyi G, Wu Z, Huo J, Goldman ID, Owens RJ, 
            Biggin PC, Lea SM, Newstead S
  TITLE     Structural basis of antifolate recognition and transport by PCFT.
  JOURNAL   Nature. 2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 
            10.1038/s41586-021-03579-z.
  PUBMED    34040256
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 
            10.1038/s41586-021-03579-z."
COMMENT     Sequence Label: pDDGFP-Leu2d_GgPCFT
FEATURES             Location/Qualifiers
     source          1..10129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    23..44
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(59..64,65..82,83..89)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     102..124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     121..137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     121..137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     156..176
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        158..176
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        200..641
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    200..317
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     612..635
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     656..672
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     656..672
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2103..2123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2130..2840
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2841..2864
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(2894..2910)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2895..2911)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      2913..3160
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2929..2947)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3178..3197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3179..3197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3207..3224)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3207..3223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3216..3238)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3451..3470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(3871..4965)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     5201..5222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5490..6293)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6294..6509)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6533..6552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6652..6674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6712..6730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      6771..8113
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    7103..7150
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        8140..8244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8245..8313,8314..9105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8463..8482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9276..9864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9765..9784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10018..10035
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
       61 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      121 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
      181 aaaagctgga gctctagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga
      241 agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct
      301 cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga
      361 ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac
      421 cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag
      481 cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact
      541 aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac
      601 aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaaccc cggattctag
      661 aactagtgga tcccccaaaa atggctgctc catctgatcc accaactgct gctactccac
      721 cagctccacc accaccagct agaagatgtt tgccagctcc atcagttgaa cctttgttgt
      781 ttttggctac tttggccttg ggtttacaag ttccattggc tactcaatac ttgtgggata
      841 gattgggtgc tgaaagaggt tacgttggtc caaatgcttc ttctccacat ggttgtggta
      901 atggttctgg tgctgttgat ccattgagag aagaagttga agctttggtt gctcattgga
      961 atttgtgcat taacctaggt ggtttcttcg tcggtttgtt ctctgttact ttgtttggtc
     1021 catggtctga ttctgttggt agaaggccag ttttggtttt accagctgtt ggtatggctg
     1081 ttcaagctgc tgtttatttg ttggtcatgt acttgagatt gcacgttgct tacttgttgt
     1141 tgggtagaat tatctctggt ttgttgggtg actacaactt gattttggct ggttgttttg
     1201 cttccgttgc cgattcttct aatcaaagaa ctagaacctt cagagtcgct attttggaag
     1261 cttgtttggg tgttgctggt atggttgcat ctgtaggtgg tggtcaatgg cgtaaagctg
     1321 aaggttatat caatccattc tggttggttt tggctgcttc tttagctgct gcattatatg
     1381 ctgctttgtg cttgcaagaa actgtcaaac aaagaagggc tgctaagttg ttgacattgc
     1441 aacattacaa ggctgtctac aagttgtata ctgctccaga agatctgtcc tctagaagaa
     1501 aattggcctt gtattctctg gctttcttct tgttggttac cgttcatttt ggtacgaaag
     1561 acttgtacgt cttgtacgaa ttgggttctc cattgtgttg ggcttcagat ttgattggtt
     1621 acggttctgc tgcttcatac ttggcttatt tgtcatcttt aggtggcttg aggttgttgc
     1681 aattgtgttt ggaagatact tgggttgccg aaattggtct gatctctaat attgcaggtt
     1741 tggtcgttat ttctttggct acaactactc cattgatgtt taccggttac ggtatcatgt
     1801 ttttgtctat ggctgctacc ccagttatta gagctaaatt gtctaagctg gtgtccgaaa
     1861 ctgaacaagg tgctttgttc gcttctgttg cttgtgttga aggtttgtgt tctttagttg
     1921 ctaccggtgt tttcaactcc ttgtatccat ctaccttgca tttcatgaga ggtttcccat
     1981 ttttgttcgg tgccattttg ttgttgattc cagctgctat tatgggttgg atcgaaatcc
     2041 aagattccaa cttgcaatac tcccatttct ctgatgcttc atcttctcca gctgatggtg
     2101 gggaaaattt atattttcaa ggtcaatttt ctaaaggtga agaattattc actggcgttg
     2161 tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg
     2221 aaggtgaagg tgacgctact tacggtaaat tgaccttaaa atttatttgt actactggta
     2281 aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt caatgttttg
     2341 cgagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg ccagaaggtt
     2401 atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc agagctgaag
     2461 tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt gattttaaag
     2521 aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac aatgtttaca
     2581 tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga cacaacattg
     2641 aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt ggtgatggtc
     2701 cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc aaagatccaa
     2761 acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt attacccatg
     2821 gtatggatga attgtacaaa caccaccacc atcatcatca tcattaactg caggaattcg
     2881 atatcaagct tatcgatacc gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca
     2941 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3001 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     3061 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     3121 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc
     3181 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3241 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3301 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3361 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3481 ccacgttcgc cggcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt
     3541 ttttagagaa aatcctccaa tatataaatt aggaatcata gtttcatgat tttctgttac
     3601 acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt
     3661 tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc
     3721 ctttttctcc ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga
     3781 acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat
     3841 gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc
     3901 gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc
     3961 tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa
     4021 tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt
     4081 tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc
     4141 tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc
     4201 ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt
     4261 tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt
     4321 agggaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa
     4381 aacattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa
     4441 agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc
     4501 gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct
     4561 gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag
     4621 agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat
     4681 ttttagtaaa ccttgttcag gtctaacact accggtaccc catttaggac cacccacagc
     4741 acctaacaaa acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac
     4801 acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt
     4861 ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc
     4921 aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattacaa tggtatatcc
     4981 ttgaaatata tataaaaaaa ggcgccttag accgctcggc caaacaacgc cggctttccc
     5041 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5101 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5161 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5221 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5281 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5341 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5401 cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
     5461 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
     5521 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
     5581 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
     5641 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
     5701 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
     5761 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
     5821 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
     5881 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
     5941 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
     6001 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
     6061 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
     6121 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
     6181 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
     6241 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
     6301 cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
     6361 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     6421 tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa
     6481 aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc agtacaatct
     6541 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     6601 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6661 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     6721 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagtat gatccaatat
     6781 caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag
     6841 aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat
     6901 cacaggaggt actagactac ctttcatcct acataaatag acgcatataa gtacgcattt
     6961 aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag
     7021 atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag
     7081 cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagaaagta
     7141 taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac
     7201 caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca
     7261 ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca
     7321 tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc
     7381 tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa
     7441 aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac
     7501 cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt
     7561 atgcgcaatc cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca
     7621 cccgcagctt cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa
     7681 gagaaaatag acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt
     7741 tatcaagaga ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg
     7801 ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt
     7861 tgttggtaaa atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     7921 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga
     7981 ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca
     8041 gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca
     8101 cagatgcttc gttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaag
//
