LOCUS       sequence-327084-        7708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sMHT-113 fused to FKBP/FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ
  TITLE     A Split Methyl Halide Transferase AND Gate That Reports by 
            Synthesizing an Indicator Gas.
  JOURNAL   ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 
            10.1021/acssynbio.0c00355. Epub 2020 Oct 26.
  PUBMED    33104325
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00355"; journalName: "ACS Synth Biol"; date: 
            "2020-11-20- 20"; volume: "9"; issue: "11"; pages: "3104-3113"
COMMENT     Sequence Label: pDH011
FEATURES             Location/Qualifiers
     source          1..7708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             148..180
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(571..573,574..897)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MMVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     promoter        910..944
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             962..1774
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             962..1774
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1866..2321)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2003..2024)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2215..2234
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(2358..2405)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2401..2419
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(2472..2489)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3194..3216
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    3231..3255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3256..3274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3288..4103)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3288..4103)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4095..4114
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4225..4813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4714..4733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4967..4984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4999..5138
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5064..5083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5224..5246
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5240..5431)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6240..7322)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7284..7303
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7323..7400)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7606..7625
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt
      181 gcgggatcca tgagcaccgt ggcgaacatt gcgccggtgt ttaccggcga ttgcaaaacc
      241 attccgaccc cggaagaatg cgcgaccttt ctgtataaag tggtgaacag cggcggctgg
      301 gaaaaatgct gggtggaaga agtgattccg tgggatctgg gcgtgccgac cccgctggtg
      361 ctgcatctgg tgaaaaacaa cgcgctgccg aacggcaaag gcctggtgcc gggctgcggc
      421 ggcggctatg atgtggtggc gatggcgaac ccggaacgct ttatggtggg cctggatatt
      481 agcgaaaacg cgctgaaaaa agcgcgcgaa acctttagca ccatgccggc ggccgcgggt
      541 gcttctggtg gtggttcttc tggtggtcac atgatggtgc aggtggaaac catcagcccc
      601 ggagatgggc gtacctttcc gaaacgtggc cagacctgcg tggtgcatta caccggcatg
      661 ctggaagatg gcaagaaatt tgatagcagc cgggaccgca ataaaccgtt caagttcatg
      721 ttgggcaaac aggaggtcat acgcggctgg gaggaaggcg tggcgcagat gagcgtgggc
      781 cagcgtgcga aactgaccat ttctcccgac tatgcgtatg gcgcgacagg ccaccctggc
      841 attattccgc ctcatgcgac cctggtgttt gatgtggaac tgctgaaact ggaataatga
      901 ggatcctgat ttacggctag ctcagtccta ggtatagtgc tagcaggagg tgtactagtt
      961 gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc
     1021 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1081 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1141 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     1201 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1261 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1321 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1381 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     1441 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     1501 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     1561 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     1621 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     1681 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     1741 gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat
     1801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1861 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     1921 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     1981 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2041 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2101 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2161 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2221 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2281 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc caatccggat
     2341 atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
     2401 gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca
     2461 gccggatctc agtggtggtg gtggtggtgc tcgagtaagc ttttagttgt tcattttttt
     2521 ccagcgcgcc agtttttctt tgcctttgcg cggttccacc gccagatcgc tataatcttc
     2581 caggctcagc tgtttaaagc ccagcggcac cagcactttt tcatattcgc tttcgctcac
     2641 gctaaacggc gggccgcctt catggttggt aatcggatac atcagggtaa tcagttcgcc
     2701 atccggtttc agcagttcat acatcgcttt gccccacgcc gggcgcattt tcggatcaat
     2761 cgcgcaaaaa aacacataat caaaaataaa atcaaacggc tgttccgggc gccaggtaaa
     2821 cacatcttct ttcacaaagc taaagcagct gctgtttgcg gccgccatgt gaccaccaga
     2881 agaaccacca ccagaagcac cgctaatacg ccgaaacaca tgatagtaca aatcccacgc
     2941 ctgggtcagg tctttcacat tgccgctttt catatactta cggcaccact cctgcgcctc
     3001 cataagatcg cggccatacg cctgattaaa gctggtttct ttcagggtct gcgggccacg
     3061 ttccatcatc gcatgcagcg gttccagaac ttcgaacatt cccttcacgt tacgttcgcc
     3121 aaaatacaaa cggctcgctt cttccaggcc ctcatgccac atctcgtgcc acagaatcgc
     3181 caccatatgt atatctcctt cttaaagtta aacaaaatta tttcttgagg ggaattgtta
     3241 tccgctcaca attcccctat agtgagtcgt attaactgca gggatcctta gaaaaactca
     3301 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     3361 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     3421 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     3481 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     3541 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     3601 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     3661 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     3721 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     3781 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     3841 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     3901 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     3961 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     4021 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     4081 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     4141 tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4201 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4261 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4321 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4381 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4441 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4501 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4561 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4621 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4681 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4741 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4801 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4861 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4921 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4981 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5041 acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     5101 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     5161 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5221 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     5281 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     5341 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     5401 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     5461 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     5521 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
     5581 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
     5641 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     5701 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     5761 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     5821 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     5881 cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     5941 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     6001 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     6061 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     6121 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     6181 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct
     6241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6301 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     6361 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     6421 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     6481 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
     6541 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
     6601 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
     6661 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
     6721 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
     6781 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
     6841 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
     6901 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
     6961 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
     7021 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
     7081 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     7141 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     7201 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     7261 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     7321 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     7381 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
     7441 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     7501 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     7561 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     7621 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     7681 gaggatcgag atctcgatcc cgcgaaat
//
