LOCUS       sequence-327085-        8702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite Gateway (attR4-attR3) vector for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
  TITLE     Combinatorial assembly of clone libraries using site-specific 
            recombination.
  JOURNAL   Methods Mol Biol. 2014;1116:193-208. doi: 
            10.1007/978-1-62703-764-8_14.
  PUBMED    24395366
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1116:193-208. doi"; pages: " 
            10.1007/978-1-62703-764-8_14"
COMMENT     Sequence Label: pDest-659 (attR4-attR3)
FEATURES             Location/Qualifiers
     source          1..8702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1931..2054
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2095..2400)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2197..2216)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2720..3400)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     3315..3334
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3454..3460,3461..3478,3479..3484))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3509..3632)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3651..4239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3704..3724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4122..4133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4290..4310)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4295..4624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4475..4610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4672..4719
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4738..5136
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5223..5456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5528..5649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5565..5584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5619..5638
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5689..5824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5844..5863)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5845..5863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5873..5890)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5873..5889)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5882..5904)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6031..6486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6118..6137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6328..6349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6512..6616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6617..6685,6686..7477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6835..6854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7648..8236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8137..8156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8390..8407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8524..8545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8560..8565,8566..8583,8584..8590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8598..8614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8603..8625
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8622..8638
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8622..8638
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8657..8677
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8659..8677
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgtccaat caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc
     1981 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     2041 atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat
     2101 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
     2161 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
     2221 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag
     2281 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
     2341 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
     2401 ttcaccagcc cctgttctcg tcggcaaaag agccgttcat ttcaataaac cgggcgacct
     2461 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
     2521 tctgcatggt tgtgcttacc gaaccggaga tattgacatc atatatgcct tgagcaactg
     2581 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
     2641 atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac
     2701 gcatactgtt atctggcttt tagtaagccg gatcctctag attacgcccc gcctgccact
     2761 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     2821 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2881 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2941 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     3001 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     3061 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     3121 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     3181 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     3241 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     3301 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     3361 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     3421 aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg
     3481 taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
     3541 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     3601 cgtttctcgt tcaactttat tatacatagt tgatgtggcg gccgctcgac aatcaacctc
     3661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3961 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4021 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4081 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4141 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4201 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattctg cagatatccg
     4261 gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4441 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4561 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4621 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     4681 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     4741 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     4801 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     4861 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     4921 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     4981 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5041 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5101 gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt
     5161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5221 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5461 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5521 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5641 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5761 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5821 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5881 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5941 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6001 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6061 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6481 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6541 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6601 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6781 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6841 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7141 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8341 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8401 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8461 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8521 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8581 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8641 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8701 tt
//
