LOCUS       sequence-327116-       13307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins (except TniQ) with crRNA 
            targeting PmcPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS19: pHelper(PmcCAST)_PmcPSP1_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..13307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        288..306
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    307..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      4230..4277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4423..4441
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4442..4466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9790..9808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9809..9833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9942..9986
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      10038..10085
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10206..10997)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(10998..11089)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11137..11875)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(12085..13167)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13129..13148
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13168..13245)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(13291..13307,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtgagtc gtggctttat gccgttaggc gttgctcaag agaagtcatt
      121 taataaggcc actgttaaac gtaggtgagt cgtggcttta tgccgttagg cgttgctcaa
      181 gatccgaatt cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta
      241 agtcgaacag aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac
      301 tataggggaa ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga
      361 aggagatata catatgacag ctatattaat tcaaagtgag gaatatgtag acttaacatt
      421 taagctaaga ggcgcaccga ttcctcttga caacggttat cttacctacg ctgccttatc
      481 ccgtatttgt cccccactac atgaactaaa gtctattggg attcatccga ttgctggaat
      541 accgaccagg aacaatttgc ttgagctaac tgctcaatct cgtctaaaaa ttcggattta
      601 tcatcagcaa attcctttaa tttatcctta tttagcgggt caagcttttc atataggtca
      661 aaatttttat caactagata tccccgacta caaaccgtta atttcttcag aaagtgtcta
      721 ttccagatta gtgataatta aagggttcca agactctact aactttattg aagctgtgca
      781 acggcaaatg gataatttag gaatacaagg aaaaattgaa cttcttaccc gacaagatgg
      841 aacacctcaa cgaagacaat taaccatcaa taaagaaggg aaacagttta aagttagagg
      901 atttggcgta aaaatcagtg aactcaatcc tgaagattcc ttaaccttgc aagaacaggg
      961 aattggcggg aaacgaaaga tgatgtgtgg aatatttgtc ccagcgactc gcagcaagga
     1021 agaagaggaa acctgatgat agtctctacc caacccaaaa tttccctatc tctccatgct
     1081 gcggatacga caataatgca ccgcgtcgga atgaccggac tttacatgac tttaaagcga
     1141 ttggaaaagc aatatccctt atctcgtcaa cggggaggac atatatcatg gtttttgact
     1201 gctgatacta ttgaattatt ctgggaagga agtgatttca tcgccttatc ttggttaatt
     1261 aatgagtctt ttcaattaga tgatacaggt ttaattcatt tagtaggatt agacaatgat
     1321 cgaatagatt taaggcagaa aatccatatt catgagggaa tatgcggtgt ttttctacgt
     1381 cttaataagt tttatcaagc aggagagata attaacactg aactaaggtt tgaagaaaaa
     1441 caagtcgagt atcaatataa atccctaact tggtacgcac atcaaacctt tgcagagaag
     1501 ttatgcgagg cagataccca acaactcaga catgattaca ttcaaataac cagttggttg
     1561 tatttagggg gaattgttcg ccatgctcgg acgcagaata ctacaaaact agaagagaaa
     1621 cctgaatacg ctttagcatt attatttgta ccagtagttt gtcattattg cttacttcat
     1681 ataccatcag aagacttaaa agaaaggaaa ccccatcgct acttggtggt gattccagaa
     1741 ataaaggatt ttgaagacgc ttctcaaagg agatggcgat tacagcaatt agaaactaaa
     1801 cagtttcatg ttagtagcct tggggaagca ggattacttt attacagctt agacgatatt
     1861 cagcctgaag tggcctacta tcaagcttgt caagtttggt tatatgaaaa aacaaataaa
     1921 gcttctcgtc aacggacatt gatgagtata gaagaaatta aaattgataa gaatatttta
     1981 atcacttatc aacaggtgca aaaatacttt aaaactaatt atcagataat taaatataag
     2041 caaattttta ttaaggtcaa tccgattcgc tccctgattg ctgataattt agtgaaagga
     2101 atccattggt ggtctaattt ttgggaaaaa ttggtgatag aggattcaaa agaatattta
     2161 tttaaccagt tattttccaa tcgagaagga ttcatcataa tggcagagaa tagtgaagaa
     2221 gacaagcagt atcttatttt tattaaggta tttcagcaag ctatgaaggg caactttgct
     2281 aaaatttatg ccaaaacaga ggaaggtaaa gatcctccaa ttaagaagaa agttgaacga
     2341 ttaagggcag agctaaacta ttgttatgat gaattgtcgt ttaaggaata tttgtctgac
     2401 tttttagtaa gagggggatt aaataagtat tttaatgagc atcaggaaga gattgcacta
     2461 ctaattaaaa aatcaccttg gcaagaaata agaatttggt cattgttagc gatcgccagt
     2521 tataaaccca aagataaact cactaatagg gatgatagtt cattgtcaaa caatcaaaaa
     2581 ctagaagaag tgaatgatga ctcagaagaa gaatgacagt aatatcccaa attattatct
     2641 ttacggaaca gtcctcactc gttatggttt agcttcctta aatcatgaca ttagacgagg
     2701 aaataagact attctgcaaa aaggctattg gaataatggt aaaattcatt cttttgtagg
     2761 ttcaagtgca attcgttggg cgttacgttt ttatcttcaa aagcaaggtt atttagttaa
     2821 tcgagtctgg gatgaggaag aacatattaa tcggttaaca agtgaagatt tcgatccaga
     2881 aaaattttat gatgatgata tttttgggtt tgctttatta gagtctgctg aaacagagga
     2941 ggacacttca acaacaaaga gaaaaaagaa acaaacaaag accagcactc ctaatcagcg
     3001 aatgggtgct ttagggatga atatggctgt ttcgctaacg ccttatgatg gtgcagttaa
     3061 gttaggggca aaaagtggca gagagaagga tagtacatct ctccatttta cagaatatca
     3121 tgcaactcga tatcaatatt attttggaat tgatgccact catctcaaag atttttcgcg
     3181 gattttacct atgatagatg ggattatgaa tctgcccaag gtaggaggga gtagtaatat
     3241 ttttaattat cctttctgtc ccgatagttt ggtatttcaa tggacaaatc attttgcttc
     3301 atatatttcc tattgctttg aatattgcga tcctaagagt aaggaagcaa aactttcaca
     3361 agagtttata gatgaagtgg aatgcggaca gattgaccct agtaaattgt ggattggggg
     3421 aacaattgtt aaggatttac agcaattgga taattttgaa agttcccctt tgaacaaggc
     3481 gcatatttat cgtaatcgaa atgaaatgat tgaagctttg aaaacagtca ttaaaagaga
     3541 tttgggcttg gaagaatcaa aatgattgca ccattaattc tctatttgga tgttccattt
     3601 acgacttttc gggagtccca tgcaagggaa atgggaaaaa cctatcctgt tcctcctcca
     3661 gcgacagtgt atgggatgtt gttgtctttg gtaggagaaa cgaatgtcta tcgccactgt
     3721 ggggtagaat tggccattgc aatgttatct agccccaaaa agtcgcgaat tttgcgtcaa
     3781 atgagacggt ttaagaatgc tgattttagc catccagaga atgttattcc ctgttatcaa
     3841 gaaattttgt ctaatctgaa gtgtttgatt tgggttcgtt ctgacgagga aaagatacaa
     3901 ccaagtttaa gggagaggat acagttagcg tttgaccatc ctgagctagt aagacggttt
     3961 ggttgtttat ttttgggaga aagtgaccag ttgattaaaa cgatcaaact tgcaagggaa
     4021 gattatctag agggagtgag acagtgggcg attagagata atcgaggcag gttaacttta
     4081 ccctattggg ttgaccatgt ggggtcaaga aatacccgat ttttgaggta tcggattgag
     4141 gaaatggata gattatcacc tcctgattta gcctggacaa tggttcaatc tccgatttga
     4201 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     4261 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     4321 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctcgg
     4381 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag
     4441 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg
     4501 agatatacca tgaaagagca atttgaacaa tgggttgctc gccttcaaat atccgaggca
     4561 gggcaaaaaa ttattgagaa agttcgctca tctgagccat cccgtcgcgt tggtggcggt
     4621 agcaagaatg tttcgggacg ctaccctagt cgcaagatgg gagtcacgat tcaatttgag
     4681 tctcatcggg ttgaactgcc aattatctat cagttagaac atgataaaga tgttctagaa
     4741 ttctacgatc aaccaccgca aattaaactt gactatcaag caagtaacgg acgtagatta
     4801 ggcgttttac acaccccaga tttatttgtc atccgaacca attcggctgg ttgggaagag
     4861 tgtaaaactg agcaggattt gaaaaaacta gcagagaaaa accccaatcg ttacttttac
     4921 tctgaagaaa ataatcaatg gcattccttg ccaggagaag tttatgctaa ccagtttggg
     4981 ctttatcacc gcattcattc agatcgcgaa atcaattggg ttctgcaacg taatttgcaa
     5041 tttttagaag attattatcg ctcagaatct ctagtagtgg aagaagcgat cgctcaatca
     5101 ttacttgcaa ttgtgtcatc tcaaccagga atcactttag cagaattact caatcggtca
     5161 acaggagcta aatctgacga tatctacacc ttaattattc aagagcaaat ctatattgat
     5221 ttaaatgcta caccactagc tgaaccagaa agatgtctaa tcttccgtga cgagcaaact
     5281 gctagtgctt atagattaat ggttgagcaa ccaagtgtaa gtcttccggc tatctctcct
     5341 gtagtcaata ttgttactgg agcaactgtt agttgggacg gcaaaggttt aaatatcatc
     5401 catgttggag aaacagaggt tatacttggc gcagaaaata atcagttaat tgaactcaaa
     5461 aaagctatat ttgagaatct ggttcgccaa ggaaaaatca caagtctcca aacaccagaa
     5521 aaaacagcta ttagcacaga atcttggcag cgatttcatc aagctagtcc tgaagaccaa
     5581 gcagaagccc ttgaacgcta caaaactatc gaaccttact taaacggaca tcctccagaa
     5641 aacgaaacaa taccagcccg taccattcgt cactggaaag ctaaatattt aattgcaatt
     5701 caaaaatacg gttgcggcta cattgggctt ttgtcccatc gcagtgtcaa aggtaatcga
     5761 cagcgcaagc tgcccgaaga caccttagca attatggaga agtttatttt agaagactat
     5821 gaaactctta aacaaaaaag aatgtgggag gttcacgcgg ctttagttcg agcttgcgaa
     5881 gaagctggtg taattgctcc gagctataaa gcatttacca aagaagtaca acgccggact
     5941 ggttacgaac agactaaaaa acgccaaggt cgtcgtgccg cttatcagca tgaatccttc
     6001 tattgggagc ttgctataac taccccaaga cacggcgatc gcccttttga aattgggcat
     6061 atagatcata ctgagttaga tgtagagcta gtttgctctg atacaggtcg caatcttggc
     6121 agaccttggg caacatttct cgtagatgct tattcacggc gattgctggc agtttaccta
     6181 acgtttgact caccctcata tcgttcttgc ttaatggtgc taagaatttg tgtcaagcgt
     6241 catggcaggc ttccccaaat tgtagtggta gataacggtg cagagtttca cagcgtttac
     6301 tttgaaacac tactagctac ttttgaatgt acaaaaaagc aaagaccccc agccaaagct
     6361 cgttttggtt cggtttgcga acgcctattt ggaacttcca atactcaatt tgtccataac
     6421 ttacttggta atacacaaat cactcgtaat gtccgccaag tcactaagtc agttaaccct
     6481 aaaaatctag ctgtatggac acttggttta ctttatgaat atctttgtgc ttgggcttac
     6541 gaagtttatg atacagacga acatccggca cttttccaga gtccgaggga tgcgtttgct
     6601 gcgggtatgg cgattggagg cagtcgggtt caccgcatga ttccttacga tgaaaacttt
     6661 cagattctta ccctacctac aacctcagag ggaaaagcaa aagttcaagt tggacgcggc
     6721 ataaaaatta actacatata ctactggtct aatagtttcc gcgatcctca aattgaaaat
     6781 acctctgtgc agataagata cgacccgttt aatattggca tcgcttacgc ctttgttcga
     6841 ggacagtggg tacagtgtat ctctcaatat tatgcagaac tccaaggtcg ttcagaaaaa
     6901 gaactgaagt tagctagtat cgagttacgc aaacactatt caaatcatac acaacaatct
     6961 aaagtttcgg ctaagaattt agcagaattt ttagcatcgg tagaagctca agaagctcta
     7021 ttagaacagc gttcctatga tgcagaagtt aaggaagtct ttagggtaat cgagggtgga
     7081 aaagcgacaa caagccgtaa cgaagaacca aaacttattc aagttacttt tgccaatacc
     7141 gattttcaag cggatgaaga tgaagcgatc gtcccagaaa cacttgtagt gtatgaggag
     7201 ttttaactat gacaaaatct actggatttc cgcttgaact cctcacacgg cctgctacag
     7261 aaaggcttgc ttactttgaa aattacaccg tagcgcatcc tcgacttaaa gaagtctatg
     7321 aaattctcat gcgaacgatt gccgaaccag caggagcttc atttatcttt gtttacggtg
     7381 ctagtggtgt tggtaaaaca actttgcgac tccgcgttga gcagaaatta acagaactag
     7441 cattacctaa attggaaagc gatcgcgctc gtgttcctgt tgttggtatt gaagctatag
     7501 cgccagaatc gagatacttc aactggaaag aatactacac acgcgcctta attactttgg
     7561 aagaaccatt aatcgatcac aagtttgatt acggtgtgcg tggtatctct cgcgataatt
     7621 ttggcaaaat taatgttgag tcgaaagtag ttgcacctgc tctacgcaga gctttagaaa
     7681 atgctctcat ccatcgtcat ccagacgtat tttttgtaga tgaagcacaa cattttggta
     7741 aggtggcaag cggatacaaa ctacaagatc aattagactg tctcaaatct ttagcaaata
     7801 tgacaggcat cttgcactgt ctattaggaa catacgaact acttacattc cgtaatttga
     7861 gcggtcaact ctcacgtcgt agtgttgata ttcatttccg ccgttattgt gctgatagcc
     7921 cagaagatgt acaggctttt aagagtgtgt tgttaacttt tcaacagcat ctgccactag
     7981 cagaaactcc aaatttagtc gatcattggg aatattttta cgaacgtact ttaggatgta
     8041 ttggcacact gaaagattgg ctgaagcgtg ttcttagtga tgctttggat cgagaagcaa
     8101 caacaatcac tctcaaagat ttacaaaaac gtgctttgtc ggttgctcag tgtcagaaaa
     8161 tgttcaagga aattcaagag ggtgaacgac aattatctga aacagaagca gatgtgcaaa
     8221 acttacgctc tgcattagga cttggcgcaa aaccaattgt gctacccgaa gaaaccccaa
     8281 aaactactcg tccgccagga aaggttggca aacgcaaacc taaaagagat cccatcggag
     8341 tgcagcaaga tgttagctag tgtattagaa acatacgaat cttggaattt aaaaaagcca
     8401 ttaattccac aacgtagtcg cctctatcaa cctcaacctg taggtattgg gactccttac
     8461 atagagagtt taacaggtta tatcacacgg atagcagagt tgcatggagt tcttccagga
     8521 gtcttgatga ctagagaaat tgctccgctt gtcaataaaa tatatttcca aaatggagct
     8581 aatcgaggtt ttcgtgaaat attcaaccgt agtcaagcgt taaatggcat gggagagatg
     8641 gcagcagatt tagttcaagt gttgcaaaaa ttgacgctta gagatgactt gcgtttttta
     8701 accatgctgt tttggtctaa cattttgact ccacgcaatt tatttcgtac cagaaaagct
     8761 tggtgtccaa tttgttatca agagcggcat caaaatgggc tggtagtcta tgagcaattg
     8821 ctttggacaa ttaacctaat tacaatttgc ccgcagcatc aaaaaccctt ggttgaactt
     8881 tgccctcact gtaatcatga atcacctttg ttaaactggc gctcaagacc tggttactgt
     8941 tcaaagtgtg gagagtggtt aggcgcaaat caatgcctta agacatttac agatggtgaa
     9001 ggatcaataa aattacagtt ggagtggcag tattggactg ccaatgttgt aggagagcta
     9061 attttagctt ctcaatgctt tgaatctgcc ccatcaaaag aaaatattac aaaatcatta
     9121 aatatagtta ttgataaagt tgcggaaaat aatgcagctg cattttctcg tcttattgga
     9181 gtaccaaaaa atagtttgtg gatgtggcag tctactaaaa ccttgccaga gctaaataca
     9241 cttctgaaaa tttgttacga attagagata tcgttggtag aatttcttac tccaaaaaat
     9301 ctaattacta aatcttttac aaaaatctcg cagaagcacc tccaactttc tcgcacacca
     9361 agggtatctc caaaatcttt cgaccaatat caagtaaaag atgctttatt agcaatactt
     9421 gcaggtaatg aagaaccacc accaacaatg gaagaagttg gaaagcgttt aggtcatcac
     9481 aacagaacta tttctagaca ctttccagat ttgtgtagtg ctatttctgc taaatgtcgt
     9541 aattacaata aagcttgccg tctcaaaagc atagaaaaac tttgcagcga agttagagag
     9601 attgtcctta gccttaatgc tcagggtgtg tatcccacag aaggtcgtgt ttgtgaatta
     9661 atgccaaatc cggggtgttt tagatataaa caagtccgcg ctgcttttaa tgatgcacga
     9721 cgtgagtttg gtttgtaact taagtcgaac agaaagtaat cgtattgtac acggccgcat
     9781 aatcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccatctt
     9841 agtatattag ttaagtataa gaaggagata tacatatggc agatctcaat tggatatcgg
     9901 ccggccacgc gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc gctgctgcga
     9961 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg
    10021 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
    10081 ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtgccct
    10141 gaaccgacga ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt
    10201 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
    10261 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
    10321 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
    10381 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
    10441 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
    10501 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
    10561 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
    10621 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
    10681 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
    10741 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
    10801 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
    10861 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
    10921 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
    10981 atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta tcagggttat
    11041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca
    11101 ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta
    11161 cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc
    11221 cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt
    11281 ccggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc
    11341 gacaggactt aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt
    11401 ccgaccctgc cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc
    11461 tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg
    11521 ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact
    11581 tgagtccaac ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga
    11641 gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc
    11701 ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta
    11761 agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt
    11821 tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11881 tactgaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
    11941 ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag
    12001 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
    12061 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    12121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
    12181 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
    12241 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
    12301 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
    12361 cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
    12421 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
    12481 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
    12541 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
    12601 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
    12661 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
    12721 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
    12781 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
    12841 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
    12901 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
    12961 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
    13021 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
    13081 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
    13141 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
    13201 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
    13261 ctcccttatg cgactcctgc attaggaaat taatacgact cactata
//
