LOCUS       sequence-327127-        2973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target plasmid containing PmcPSP1 and attachment site for PmcCAST..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS30: pTarget(PmcCAST)
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(74..619)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(154..173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1236..1255)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(1686..2345)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2260..2279
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(2913..2930)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
       61 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
      121 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
      181 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
      241 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
      301 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
      421 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
      481 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
      541 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
      601 cagaccaaaa cgatctcaag aagatcatct tattaatata cgtagtatct acgatacgta
      661 gtatctacga tacgtagatc atggagaagt catttaataa ggccactgtt aaacgtagat
      721 atatctacgc gtagatatat ctacgatacg tagcggccga cgcgctgggc tacgtcttta
      781 atgggcagcg cccacatacg cagcgatttc caacagcttg aggcggaagc tttaatgcgg
      841 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
      901 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
      961 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     1021 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     1081 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     1141 ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac gcatcgtggc
     1201 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1261 ggaagatcgg gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc
     1321 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     1381 agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat
     1441 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac
     1501 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     1561 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     1621 ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa
     1681 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1741 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1801 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1861 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1921 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1981 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2041 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2101 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     2161 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2221 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2281 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2341 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggggcg
     2401 gttagctcag ttggtagagc gcctgcctta caagcaggat gtcatcagtt cgagtctggt
     2461 actgcccagt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     2521 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     2581 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     2641 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
     2701 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
     2761 aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga
     2821 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     2881 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     2941 gttcgactgc ggcgagcgga aatggcttac gaa
//
