LOCUS       sequence-327131-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insertion of MAU2 degron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Zhu Y, Denholtz M, Lu H, Murre C
  TITLE     Calcium signaling instructs NIPBL recruitment at active enhancers 
            and promoters via distinct mechanisms to reconstruct genome 
            compartmentalization.
  JOURNAL   Genes Dev. 2021 Jan 1;35(1-2):65-81. doi: 10.1101/gad.343475.120. 
            Epub 2020 Dec 17.
  PUBMED    33334824
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2021-01-1"; volume: "35(1-2):65-81. doi"; pages: " 
            10.1101/gad.343475.120. Epub 2020 Dec 17"
COMMENT     Sequence Label: px330 MAU2 gRNA
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..290
                     /label=chicken beta-actin promoter
     intron          291..518
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      530..539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             539..604
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             611..631
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             656..4756
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             656..4756
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4757..4804
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4832..4849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4838..5045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5054..5194
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5054..5183
                     /label=AAV2 ITR
     rep_origin      5269..5724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5356..5375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5566..5587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5741..5760)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5860..5882
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5920..5938)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6006..6110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6111..6179,6180..6971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6329..6348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7142..7730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7631..7650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7792..8040
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7792..7812
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7963..7982
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8061..8136
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(8233..8507,1..11)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
       61 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      121 gggggggggg ggngcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      181 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      241 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
      301 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
      361 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
      421 attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta ttaatgttta
      481 attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga
      541 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga
      601 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
      661 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
      721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1441 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     2821 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     2881 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3061 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3361 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     4741 tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     4801 aaagtaagaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4861 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4921 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4981 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     5041 ggggagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     5101 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     5161 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     5221 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     5281 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5341 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5401 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5461 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     5521 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5581 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     5641 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5701 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     5761 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5821 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5881 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5941 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6001 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6061 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca
     6841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta
     7801 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     7861 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     7921 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     7981 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     8041 ggaggcggct tgttgttgtg gttttagagc tagaaatagc aagttaaaat aaggctagtc
     8101 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata
     8161 gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc
     8221 tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8281 cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8341 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     8401 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc agtacatgac
     8461 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcg
//
