LOCUS       sequence-327138-       10990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Bruning JC, Michael MD, Winnay JN, Hayashi T, Horsch D, Accili D, 
            Goodyear LJ, Kahn CR
  TITLE     A muscle-specific insulin receptor knockout exhibits features of the
            metabolic syndrome of NIDDM without altering glucose tolerance.
  JOURNAL   Mol Cell. 1998 Nov . 2(5):559-69.
  PUBMED    9844629
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Nov . 2(5):559-69."
COMMENT     Sequence Label: pBS MCK CRE
FEATURES             Location/Qualifiers
     source          1..10990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(896..912)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(937..956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(938..956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(966..983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(966..982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(975..997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1123..1579)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1261..1282)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1473..1492
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1606..1710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1711..1779,1780..2571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1929..1948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2742..3330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3231..3250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3484..3501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3618..3639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3654..3659,3660..3677,3678..3684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3692..3708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3697..3719
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3716..3732
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3716..3732
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3751..3771
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3753..3771
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3808..3824
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3808..3824
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             3843..4874
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     5218..5237
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5355..5403
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(5442..5458)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tccttttaga attgttatat tttggtgatt tatattaata atgatgttac ctgttctgtt
       61 tgtgattcac tgaatacatg gtttcagagt tgtaatgctt ggatgatttt tgtgctttac
      121 tctgcccaat ggttttggtt ggtatctgtg attgtttcat tggatatagt ttctaagaga
      181 tgtaatgttt ggnttttttt tttttttttt tttttatgta taaaatcccc tgcttgagag
      241 ctgcaaaata cactcagatt caaactgcct ctgtgtttgt ttctgtttgt caccgccgaa
      301 cccttaccca cctagcttcg aggacgctca ttccagggac ctccatacct gactgggtgg
      361 tccgtggcag gtttcaagag ttcgaggtcg acctggtcta cagagggagt ttcaagacag
      421 ttaggactac acagaggagc cctgtcttga aaaacaaaca aacaaacaaa caaacaaaca
      481 aacaaaaaac catttttaaa aattgaaccc ttacctggga aacagagggt acatggtgcc
      541 ttcctcctct gagagagcac tgtgacatct gaggtggaca gagccagctg tgcaaggagg
      601 gagcagagag aagagcgagg ccctgaggaa ggaatgctga ggggagactg gaataaccca
      661 aggagcgcag gaaaaagatg ccattgggca aggagttcat gggagccact gagggtcaga
      721 ggcagagaga ggcaggcccc ggccatgtca taggcttgcc agggagcaca cgccaccctc
      781 tgagaacccc tcttgggtca ttcaactttg tttttccaga tgggtttatc tgtagccctg
      841 gctgttctgg aactctctct atagaccagg atggggaatt cgatatcaag cttatcgata
      901 ccgtcgacct cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg
      961 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1021 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1081 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata
     1141 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc atttttttaa ccaataggcc
     1201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     1321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     1381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     1441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     1501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     1561 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1681 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1741 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1801 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1861 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1921 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1981 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2041 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2101 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2161 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2221 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2281 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2341 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2401 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2461 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2521 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2581 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2641 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2881 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3481 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3541 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3601 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3661 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3721 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     3781 ctggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcagccg
     3841 atatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
     3901 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
     3961 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
     4021 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
     4081 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
     4141 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
     4201 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
     4261 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta
     4321 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
     4381 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
     4441 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
     4501 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
     4561 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
     4621 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
     4681 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
     4741 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
     4801 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
     4861 aagatggcga ttagccatta acgcgtaaat gattgctata attatttgat atttatggtg
     4921 acatatgaga aaggatttca acatcgacgg aaaatatgta gtgctgtctg taagcactaa
     4981 tattcagtcg ccagccgtca ttgtcactgt aaagctgagc gatagaatgc ctgatattga
     5041 ctcaatatcc gttgcgtttc ctgtcaaaag tatgcgtagt gctgaacatt tcgtgatgaa
     5101 tgccaccgag gaagaagcac ggcgcggttt tgctaaagtg atgtctgagt ttggcgaact
     5161 cttgggtaag gttggaattg tcgaggctgg gtgtggcgga ccgctatcag gacatagcgt
     5221 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5281 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5341 tcttctgagg ggatcggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     5401 ttcggatccg tcgacctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
     5461 gccggcggcc tttgggaccc tgtgagcaga ccgggttcag acttaagaac tcagttgctg
     5521 ggggcttgca gtccgtcgga ccgtgggctt gaaaaagggg ggttctcccc ctgtgaacct
     5581 cagccttgca aggaaacatc aggagcccaa gttgcctgat gtggccacat cctgcctatg
     5641 tgatcctgct ggggactcag tctctctagc tggaaaagga gggcaaaacc ttttgtctaa
     5701 agccagaaaa ggacagtcac ataaacaaaa acaactacaa aaatacagga tctggggctg
     5761 gggttctgct ggcagagtgc ttgcccagca agcttaggac cctgggtaac agtttggcgc
     5821 tcagcaaatc tgggtgtggc tgctcatgcc tgaaatccca gcagttcaag gtcatcttca
     5881 tctacgtaga gagctggaga ccagcccgag tctgggctcc tacacaggtc tctatctcaa
     5941 cccaaaggaa ctgggcatga tggtccatgc ttgtaattcc aacactctga aactgaggca
     6001 ggaggattgc agtgaattca agggtagcct gggctacaga aaccctgcca ccaggaatta
     6061 aaataaaata aaataaaata aaaagatgaa taaaagcagc aaaggatctg gatcagatgg
     6121 tctgggttcg aatttcggct ttgcaactta caagcagccg cgtgactctg aggagcctgt
     6181 ttgactttcc tgttcactca tctgtaacat ggagactgtg gcatcgctga cctcttggga
     6241 gggattaaaa gacaagaaaa gcgcctcggc cagtgcctgg cacataacac aaatatttta
     6301 ttcgattgtt cttgggtctg tggtgatttg aatgagaaga gcctcagact cacatatttg
     6361 aatgtttggt ccccagtcgg tggaactgtt tgggaaggat tcggaggtgt ggccttgttg
     6421 gaggaggtgt gccattgctt gtgggctttg agatttccga aaagccaccc cattcccaag
     6481 tggctctcac tgcctctggt tgttgataaa gatgtgagct tccagcctct gctccagcac
     6541 catgcccacc tccctgccgc tccagaaccc tgagccccaa attaatgctt tctcttttct
     6601 agattgtctt ggtcatggtg tttgtcacag aaattgaaaa ccaatctgac aggctctgtg
     6661 tgaggaccct ggtaaagatt atatgtgcac acctgagaaa agccataagg gctcccctga
     6721 gattctgatc caaattagtc cagatgtggg cgtgggaatg agtctggagg cttcagccac
     6781 ttctcccagt ccctccacct ctggctgtct acaaggtcca ctttaaaagg cacggctctc
     6841 ctaagtcact gctctgggcc ccaggctgtg actagatctc tctggctttt gaatctgtga
     6901 gactgaagct ttcccaggag atctctgagt tccagtccct gactgatgag catggacctg
     6961 gcacctggca tgaagttgca gctgggattc taacagttac tgttaatatt tttgtttgta
     7021 gtgcaggctc atgtatgtgc acaggtgtgc acattccagt ggtgacctca ggtgctgcaa
     7081 cattgttatt ttatatattt attttatttt attttatttt attttatttt tatcttgagg
     7141 tagggtctct cgcaggcctg gagctaggct gcctggccat ggagccccac agatcccctg
     7201 tccccacctc cccaggggga ttctgatgtt ctcccccaca gccagctgtt ttgttttgtc
     7261 ttgtctttta aagggtgtga acactgggat ttgaactaag ccctaagctg ccccagtcac
     7321 gtcacgtgta ttttattact gttgtttttc attattaatt ttattgacta gatccctgaa
     7381 gcactgacca tgggggacat caggcctcca tcggggctgg aagggggcct cagaggaagg
     7441 tgagagggaa ggtggaccaa gctgagagaa gagtgtgggg cagacagaga cccaaagccc
     7501 ttgaagctgc tactttgtgg ctatttttag atgctcctca ccccccactg ccacctgtcc
     7561 tccctccttg ctggctcgcc ttgcactaag tcagcattgg gacaggtggg ctggggtggc
     7621 caggaggaca cacagccagg ctctgggcct caaggacacc ccgggctccc agcccagatc
     7681 tctcccttca gaaggttctg tatcctctgc taccccctcc ctcaggggca ccccaggaga
     7741 caggacaccg aggcaagacg cagaagcaga aagcttcgga atccccaaac tcatcacaga
     7801 ttctctaaga tctccaggta aactgagtca tgtagtcctt cctgccttca agatcccaga
     7861 gacagccaga cagaaggact caaaggaaag ccaggggcct gtaagagtga gagcccccaa
     7921 gtctcacaca agaaagaccc gccgggcggt ggtggcacac gcctttaatc ccagcacttg
     7981 ggaggcaggt ggatttctga gttcgaggcc aggctggtct acagagtgag ttccaggaca
     8041 gccagggcta tgcagagaac accacccccc ctgacccatc tcgaaagaga gagggagaga
     8101 gagagacaga aagagagaga tagagacaga gagacagaga gacatagaga cagaccagga
     8161 cagtgagaca caaggtcaca gagctgcaac acaggtccac atggttacaa agttaaaggg
     8221 ccactgacag gtgtcactga gacaactgtc tttgtgtggg ccattaactc tgctaaacta
     8281 agttccagcc caccctgcct tgcaaggagg ggacacttgg tggctattga gacctaggtg
     8341 tcccagacat ttgtccccac aacttgtgct gcaaaatcca caaccataac atgactgggt
     8401 gagcagaccc ttggactccc acgccccact cccaccccag tgatttctct cagtccctac
     8461 ctggctggct ggctcctgag tgtctgtctg tgctgtggag gtggtggtga cccgggggca
     8521 gcccctgtgc ccctgggtta tatagaggag cctacagggt gtgactagcc aggaggggct
     8581 gtccccaggg aggggcccct gagagcagat gagctttcag ctcgttgccc gggcaccgtg
     8641 cccaccccgg acccaggcgt gcagcttgcc cagccccatg gccttgtatg ggctgcccca
     8701 agggctgact tgctcactgg ttcctaaact aagtgctgag tctagctggc gggggacagc
     8761 tggcccttcg ccgggaacat ggaacagtaa tactttggga gtcccaggca cgtataagcc
     8821 ctggccccca agcctgttac agcctgccct cagtccccca cagccttgat tgtagccagc
     8881 accctccttc attgccttca ttgctaatgc tcaccaggct tcattgcctg gtgagcatta
     8941 gcagctggga gaaccatttt gtttgtgttt ttgaaatagg gccttactct gtagacaggg
     9001 ctagcctgaa accctatgta gtccaggctg gcctcagaca tgtaacaatc cctctgcctc
     9061 agtctcccga gtaggattac ttgcctgact ttagttattt aattgttttt ggttttttga
     9121 gacagggtct aactatgcca cccagtctgg ctttgcactc atggcaatcc ccctgcctca
     9181 gccttccaag ggtcaaggta tgtgtcattt tttgcagttg gaggttcaga accatgcatg
     9241 ccaggcaagc actgttcttg ggagccgtga ctctagccta cagcacctgg ggggaaattt
     9301 ccagtgaaag cctaggatcc ccaggcccag gaaggataca ggagacgggg gtgggggtgg
     9361 ggctgtagtc gcccatctac aggaaaggca ataactgtag ctgaaaggtt agaacccggt
     9421 ttggtttctc ccagaagccc agtgcaggct gctccaccat ggcagaattg acaggaaaag
     9481 ctacagaagc agaaaaacca ttcttgggaa aacaaacctg cggctgaggg aagtggccgc
     9541 cctcagcctg cccctcaccc tggatccacc agggacaggg ttatttttag agcgagcttc
     9601 tcctccatgg tgtacagagc ctaagaccca ggcaccgggg tgggggtgag gctcaggcag
     9661 caggtgttgg gggggggggg gcagccacat gtctgggtta attataacca ggcatctcgg
     9721 gtgtccccag gccttgcctc cttacatggg cagcctagac ccgtagtggc tgctctcatc
     9781 cctagggaga ggtgggctag gtggccccca agctgtggaa ggcagaccag agcaggacac
     9841 aatatgacag gagccttttg cacctcagct gtgaccaagg acctcagagt ccgaggaggg
     9901 gattcatgga agtgagatgc actgtggaag tttcctcttc tgcctgggag gtgccaggat
     9961 tgcccctggg gggagaccag cttctccagt attgcccccg gctccctctg ttgattctgg
    10021 agtagccagt atggcttcac tccatcttcc cccatttctg cccaggctgg tgaggggagc
    10081 ctgctctagg gggcggagaa gggaagaagt gtttaaggtg ttcctggggt ccagcctctg
    10141 tcctggcacc cgtgttcctt gtcagcatgg tcagatgagc aaaggcctcc tctgggcctt
    10201 ttacttttag cccaagacac taaaaggaat gggtttctgc cacaccacct ccagtaagat
    10261 ctgtgtgaca agttccacag actgaggcca ttgacctcaa aacgtggagg cctcctggag
    10321 atggttgtgg aactcttcgc cggagagaaa aaatattgag ccgtagttct catggcaatc
    10381 tctctgtttt gttttctctt attccttttc ttatttacat gttcctggta gcctaggcca
    10441 agatcttaag cgagaataat gggtgagatg ggcctagaga tgggccacta acatgacttt
    10501 atccccaagg aaaccaacac tttgcttttt ttggggggtg gggggttcga gacagggttt
    10561 ctctgtgtag ccctggctgt cctggaactc actctgtaga ccaggctggc ctcaaactca
    10621 gaaatcctcc tgcccctgcc tcccgagtgc agggattaaa ggcgtgtgcc accacgcccg
    10681 gctaacactt tgctttttaa atcatttaca aaactattat gataggtaca aattttccca
    10741 acaataaaat ttcatatggc tacgcgtgtg tcattggagg tgaattttgg tatatggtaa
    10801 agaaagcctt aattaggaag aattaaaata gttttaaatt acatagtgaa gatttataaa
    10861 aaaaaattag gcactagatg ctaaataata attgaccatg gggctggaga gttagttcag
    10921 tggttaagat ttactaacct gctcctggaa atctgccact agccttggac gacagccccc
    10981 actggataca
//
