LOCUS       sequence-327139-        8629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Cheng SC, Kim R, King K, Kim SH, Modrich P
  TITLE     Isolation of gram quantities of EcoRI restriction and modification 
            enzymes from an overproducing strain.
  JOURNAL   J Biol Chem. 1984 Sep 25;259(18):11571-5.
  PUBMED    6088551
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1984-09-25"; volume: "259(18)"; pages: "11571-5"
COMMENT     Sequence Label: pSCC2
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    136..150
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             309..374
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     primer_bind     complement(3446..3465)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4923..5114
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5108..5130)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5216..5356
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5271..5290
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5371..5388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5542..6130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5622..5641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6301..7092,7093..7161))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6924..6943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7162..7266)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7334..7352
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataaaaaaca tacagataac catctgcggt gataaattat ctctggcggt gttgacataa
       61 ataccactgg cggtgatact gagcacatca gcaggacgca ctgaccacca tgaaggtgac
      121 gctcttaaaa attaagccct gaagaagggc agcattcaaa gcagaaggct ttggggtgtg
      181 tgatacgaaa cgaagcattg gccgtaagtg cgattccgga ttagctgcca atgtgccaat
      241 cgcggggggt tttcgttcag gactacaact gccacacacc accaaagcta actgacagga
      301 gaatccagat ggatgcacaa acacgccgcc gcgaacgtcg cgcagagaaa caggctcaat
      361 ggaaagcagc aaatcccctg ttggttgggg taagcgcaaa accagttgat ccggctggaa
      421 atacaggaac gcacactgga tggtccttcg ttgtcgctgt gatggcgaaa ctatgaaaaa
      481 tggcaggttc ggtggatttt gacgggctaa tgtggtctgc accatctggt tgcataggta
      541 ttcatacggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg tttgttgcca
      601 tttttacctg tctgctgccg tgatcgcgat gaacgcgttt tagcggtgcg tacaattaag
      661 ggattatggt aaatcaaacg tatgttaatc tatcgacata tgtaacttta taaaataaca
      721 gtggaaacat ggattcatgt ctaataaaaa acagtcaaat aggctaactg aacaacataa
      781 gttatctcaa ggtgtaattg ggatttttgg ggattatgca aaagctcatg atctcgctgt
      841 tggtgaggtt tcaaaattag taaagaaagc tcttagcaac gaataccctc aattatcatt
      901 tcgatataga gatagtataa agaaaacaga aataaatgaa gctttaaaaa aaattgaccc
      961 tgatcttggc ggtactttat ttgtttcaaa ttccagcatc aaacctgatg gtggaattgt
     1021 agaggtcaaa gatgattatg gtgaatggag agttgtactt gttgctgaag ccaaacacca
     1081 aggtaaagat attataaata taaggaatgg tttgttagtt gggaaaagag gagatcaaga
     1141 tttaatggct gctggtaatg ctatcgaaag atctcataag aatatatcag agatagcgaa
     1201 ttttatgctc tctgagagcc actttcctta cgtccttttc ttagaggggt ctaacttttt
     1261 aacagaaaat atctcaataa caagaccaga tggaagggtt gttaatcttg agtataattc
     1321 tggtatatta aataggttag atcgactaac tgcagctaat tatggaatgc ctataaatag
     1381 taatctatgt attaacaaat ttgtaaatca taaagacaaa agcattatgc tacaagcagc
     1441 atctatatat actcaaggag atgggaggga gtgggattcg aaaatcatgt ttgaaataat
     1501 gtttgatata tcaacgactt cgctcagagt gttggggcgt gacttgtttg aacagcttac
     1561 atctaagtga tattttttat tttaataagg ttttaattaa tggctagaaa tgcaacaaac
     1621 aagttactgc acaaagctaa aaaatcgaaa agcgacgaat tttacactca gtattgtgat
     1681 attgagaacg aactgcaata ctacagagag cacttctctg ataaggttgt ttattgcaat
     1741 tgtgatgatc ctagagtaag caatttcttt aaatattttg cagtgaattt tgataatctt
     1801 ggcttgaaaa agttaatagc atcttgctat gtagagaata aagaaggttt ttctagtagc
     1861 gaagccgcga agaacggatt ttactatgaa tatcataaag aaaatggaaa gaaattagtt
     1921 tttgatgata ttagtgtttc ttctttctgt ggcgatggcg attttcgcag ttcggagagc
     1981 attgatctgc taaaaaaatc agatattgtt gttacgaatc ctccattctc gttatttaga
     2041 gagtatcttg atcaactaat taagtatgat aagaaattcc ttataattgc taatgttaat
     2101 tcaataacat ataaagaggt gtttaatcta attaaggaaa ataagatttg gcttggggtt
     2161 catctcggga gaggtgtttc tggatttatt gttccagagc attatgaatt atatggtact
     2221 gaggcgagaa ttgattctaa tggtaataga attatctcgc caaacaactg cttatggcta
     2281 actaacctag atgtctttat taggcataaa gacttgcctc ttacaagaaa atattttggg
     2341 aatgaaagtt catatccaaa atatgataat tatgatgcta taaatgtaaa caaaacaaag
     2401 gatattccat tagattacaa tggggttatg ggggttccta tcacattctt gcataagttt
     2461 aaccctgagc aatttgagtt aataaaattt agaaagggtg ttgatgaaaa agatttgtct
     2521 ataaatggta aatgccctta tttcagaatt ttgataaaaa acaaacgatt acaaaagtaa
     2581 ttgatgtttg ttagtttttt cttgagatca ttagcttccc ggatcaaccg ccctattctc
     2641 tcgctgaatc gcaaaccgaa atcacgagta gaaagcgcac taaatccgat agaccttaca
     2701 gtgctggctg aataccacaa acagattgaa agcaacctgc aacgtattga gcgcaagaat
     2761 cagcgcacat ggtacagcaa gcctggcgaa cgcggcataa catgcagtgg acgccagaaa
     2821 attaagggaa aatcgattcc tcttatctag ttacttagat attggccttg gctttatctc
     2881 aatattatat ggatcatagc tggcaactaa ttcagtccag taaatatcct caatagggaa
     2941 taatatatgc tttccattcc atcgggaaaa agttttgttc aacacaccaa gctcaatcaa
     3001 ctcactaatg tatgggaatt gttttgatgt aaccacatac ttcctgcctt cattaagggc
     3061 tgcgcacaaa accatagatt gctcttctgt aaggttttga attactgatc gcactttatc
     3121 gttttgcatc ttaatgcgtt ttcttagctt aaatcgctta tatctggcgc tggcaatagc
     3181 tgataatcga tgcacattaa ttgctagcga aaatgcaaga gcaaagacga aaacatgcca
     3241 cacatgagga ataccgattc tctcattaac atattcaggc cagttatctg ggcttaaaag
     3301 cagaagtcca acccagataa cgatcatata catggttctc tccagaggtt cattactgaa
     3361 cactcgtccg agaataacga gtggatcctc tacgccggac gcatcgtggc cggcatcacc
     3421 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     3481 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     3541 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     3601 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     3661 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     3721 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     3781 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     3841 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     3901 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     3961 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     4021 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     4081 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     4141 attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     4201 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     4261 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     4321 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     4381 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
     4441 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     4501 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     4561 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     4621 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
     4681 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     4741 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
     4801 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
     4861 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
     4921 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     4981 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     5041 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
     5101 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5161 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     5221 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     5281 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5341 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5941 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6601 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6901 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     6961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7261 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7321 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctcatgttt
     7381 gacagcttat catcgataag cttcagttga agatattaag aacagcctcg cagatgacga
     7441 atcattggga ttcccatctt ttttgtttgt tgaaggcgac accattggtt ttgccagaac
     7501 tgttttcggg ccgaccacat ccgatctgac agatttttta atcgggaaag gaatgtcatt
     7561 aagcagtgga gagcgcgttc agatagagcc actgatgagg ggaaccacca aagacgatgt
     7621 tatgcatatg catttcatcg gccgaacaac ggtgaaggta gaagccaagc tacctgtatt
     7681 tggcgatata ttaaaggtct taggggcaac agatattgaa ggggagcttt ttgactcatt
     7741 ggatatagtc attaagccaa aatttaaaag ggatataaaa aaggttgcca aggatattat
     7801 ttttaacccg tcacctcaat tttcagacat tagcctgcgg gcaaaagatg aggccggaga
     7861 tattttaaca gaacattatc tatcagaaaa aggccatctc tcagcgcctc tgaacaaggt
     7921 caccaatgct gagatagctg aagagatggc atattgctac gcaagaatga aaagtgatat
     7981 actggaatgt tttaaaaggc aggtgggcaa agttaaggat taattatcag gagtaattat
     8041 gcggaacaga atcatgcctg gtgtttacat agtaataatt ccttacgtta tcgtaagcat
     8101 ttgctatctc cttttccgcc actacattcc tggtgtttct ttttcagctc atagagatgg
     8161 tcttggggcg acattgtcat catatgcagg aaccatgatt gcaatcctga ttgctgcctt
     8221 gacgtttcta atcggaagca gaacgcgccg actggccaag attagagagt atgggtatat
     8281 gacatcggta gttattgtct atgcccttag ttttgttgag cttggagctt tgtttttctg
     8341 cgggttattg cttctttcca gcataagcgg ctacatgata cccactatcg ccatcggcat
     8401 tgcctctgca tcgttcattc atatatgcat ccttgttttc caactatata atttgaccag
     8461 agaacaagaa taacccggcc tcagcgccgg gttttctttg cctcacgatc gcccccaaaa
     8521 cacataacca attgtattta ttgaaaaata aatagataca actcactaaa catagcaatt
     8581 cagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcat
//
