LOCUS       sequence-327155-        8810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin
            receptor gene OsTIR1. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
  TITLE     Auxin-mediated protein depletion for metabolic engineering in 
            terpene-producing yeast.
  JOURNAL   Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.
  PUBMED    33594068
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-16"; volume: "12(1):1051. doi"; pages: " 
            10.1038/s41467-021-21313-1"
COMMENT     Sequence Label: pJAID8Z104
FEATURES             Location/Qualifiers
     source          1..8810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             431..1141
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1148..1279
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     terminator      1472..1742
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     protein_bind    2442..2453
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             complement(2839..2856)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             2868..4595
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(4831..4847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4831..4847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4844..4866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4855..4871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4879..4885,4886..4903,4904..4909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4924..4945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5062..5079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5233..5821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5313..5332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5992..6783,6784..6852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6615..6634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6853..6957)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7025..7043
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7081..7103)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7203..7222
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7416..7438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7430..7447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7431..7447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8561..8594)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(8805..8810,1..408)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
       61 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
      121 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac
      181 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
      241 cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      301 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      361 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
      421 aatgggatcc tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
      481 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
      541 ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc
      601 aaccttagtc actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat
      661 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
      721 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
      781 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
      841 tcacaaattg gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa
      901 gaatggtatc aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt
      961 agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa
     1021 ccattactta tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat
     1081 ggtcttgtta gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa
     1141 aagatctcca aaagacccag ctaaacctcc agccaaggcc caagtcgtcg gctggccacc
     1201 agttaggtct tacagaaaga acgtaatggt tagttgtcaa aagtcctccg gtggtccaga
     1261 agctgctgcc tttgttaaga tgttcagcga attaattaac ttccaaaatg aaggtcatga
     1321 gtgccaatgc caatgtggta gctgcaaaaa taatgaacaa tgccaaaaat catgtagctg
     1381 cccaacgggg tgtaacagcg acgacaaatg cccctgcggt aacaagtctg aagaaaccaa
     1441 gaagtcatgc tgctctggga aataaactag tattgaattg aattgaaatc gatagatcaa
     1501 tttttttctt ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg
     1561 aatatttttt atttatatac gtatatatag actattattt atcttttaat gattattaag
     1621 atttttatta aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca
     1681 ttcgatattt ctatgttcgg gttcagcgta ttttaagttt aataactcga aaattctgcg
     1741 ttcgttaaag ctttcgagaa ggatattatt tgcggccgcg attgcaagaa ttgatctccc
     1801 tcatcgatgt cataaatcag taaatataaa cattaaatac attacacgta gccacccgct
     1861 gtatagcata aaactaactt aaaattattc atatgcgttt cccggggccg aagcgttatt
     1921 gccgatattc ggtagccgat tccgctggct tttttttttt gaacgaggca aaaaagtttc
     1981 gtgactgaac ttcttcgtca ctggttttgt tgataatttt tatcgttttt tacttcgaga
     2041 ttgaaaacag tgttctgaat tttagcaacg atatcgcacc gcacgtttag cctgatcaaa
     2101 ccttatatat acctctacct taaaatatat atatatatag tattcgaatt ccgaattgtg
     2161 tgtctccgcg gttagtgatt gttatacaca aacagaatac aggaaagtaa atcaatacaa
     2221 taataaaagc tagcaaaatg gtgacttcta atgttgtcct agtgagtggt gagggtgaac
     2281 gattcaccgt agacaagaaa atcgcggaga gatccctgct attgaaaaac tatctgaacg
     2341 atatgcatga cagcaaccta caaaacaact cagattcaga atcagattca gactcggaaa
     2401 cgaaccacaa aagcaaagac aacaataacg gtgacgacga cgatgaggat gacgacgaga
     2461 ttgtgatgcc cgtgcccaat gttcggtctt ccgttttgca gaaggttatc gaatgggccg
     2521 agcaccacag agactctaac ttccctgacg aagatgacga cgattccaga aagagtgctc
     2581 ccgtggactc atgggacaga gaattcctga aagtggacca agagatgcta tacgaaatta
     2641 ttctggcggc aaactacctc aacatcaagc ccttgcttga cgctggctgc aaggtggttg
     2701 ctgaaatgat caggggaagg tctcccgagg aaattagaag aacgtttaat atcgtcaacg
     2761 acttcactcc agaggaggag gctgccatta ggcgtgaaaa tgaatgggct gaagaccgtg
     2821 gtatctttga tttgggtgct ggtgctggtg ctggtgacct tacttctatg acctatttcc
     2881 cagaagaagt cgttgaacac atcttctctt tcttgccagc tcaaagagat agaaatactg
     2941 tttccttggt ttgtaaggtt tggtatgaaa tcgaaagatt gagtagacgt ggtgttttcg
     3001 tcggtaattg ttacgctgtt agagctggta gagttgctgc tagattccca aatgttagag
     3061 ccttgactgt caaaggtaaa ccacattttg ctgactttaa cttggtccca ccagattggg
     3121 gtggttatgc tggtccatgg attgaagctg ccgctagagg ttgtcacggt ttggaagaat
     3181 tgcgtatgaa aagaatggtc gtttctgacg aatctttgga attattggcc cgttcttttc
     3241 caagattcag agctttggtt ttgatctctt gtgaaggttt cagtactgac ggtttggctg
     3301 ccgttgcttc ccattgtaaa ttattgagag aattggactt gcaagaaaat gaagtcgaag
     3361 atagaggtcc aagatggtta tcttgttttc cagactcttg tacttctttg gtctctttga
     3421 acttcgcttg tattaagggt gaagtcaacg ctggttcttt agaaagattg gtctcccgtt
     3481 ctccaaactt acgttctttg agattgaatc gttctgtttc cgttgatacc ttggctaaaa
     3541 tcttattgag aaccccaaac ttggaagact taggtactgg taacttgacc gacgatttcc
     3601 aaactgaatc ctactttaaa ttaacttctg ctttagaaaa gtgtaagatg ttgagatcct
     3661 tatctggttt ttgggacgcc tctccagtct gtttatcttt tatctatcca ttgtgtgctc
     3721 aattaactgg tttaaactta tcttacgctc caaccttgga tgctagtgac ttaaccaaga
     3781 tgattagtag atgtgttaag ttacaaagat tatgggtttt agattgtatt tccgataagg
     3841 gtttacaagt cgttgctagt agctgtaagg atttgcaaga attaagagtc tttccaagtg
     3901 atttttacgt tgctggttac agcgctgtta ctgaagaagg tttggtcgct gtttctttgg
     3961 gttgtccaaa gttgaacagc ttattgtact tttgtcatca aatgactaat gctgccttag
     4021 tcactgttgc taagaactgt ccaaacttca cccgtttcag attgtgtatt ttggaaccag
     4081 gtaaaccaga cgttgtcacc tcccaaccat tggatgaagg tttcggtgct atcgttagag
     4141 aatgtaaggg tttgcaaaga ttatctatct ctggtttgtt aactgacaaa gttttcatgt
     4201 acatcggtaa atatgccaaa caattagaaa tgttgagcat tgctttcgct ggtgactctg
     4261 acaagggtat gatgcacgtt atgaatggtt gtaagaattt gagaaagttg gaaattagag
     4321 attctccatt cggtgacgct gccttattgg gtaattttgc tagatacgaa actatgagaa
     4381 gtttgtggat gagttcctgt aacgttacct tgaaaggttg tcaagtctta gccagcaaga
     4441 tgccaatgtt gaacgtcgaa gtcatcaacg aaagagacgg tagtaacgaa atggaagaaa
     4501 atcacggtga cttaccaaag gtagaaaagt tgtacgtata cagaactact gctggtgcta
     4561 gagatgatgc tccaaatttc gtaaagattt tgtaactcga gaggactatt tgcaaaggga
     4621 agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga
     4681 agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca
     4741 caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat
     4801 ttaaatgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6961 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7021 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     7081 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7141 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     7201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7261 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     7321 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7381 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7441 ggccagtgaa ttcatttaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga
     7501 aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga agaaacatga
     7561 aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa
     7621 aacatgtcca caaaatcata taccagtaga gctgagactc atgcaagtcc ggttgcatcg
     7681 aaacttttac gtttaatgga tgaaaagaag accaatttgt gtgcttctct tgacgttcgt
     7741 tcgactgatg agctattgaa acttgttgaa acgttgggtc catacatttg ccttttgaaa
     7801 acacacgttg atatcttgga tgatttcagt tatgagggta ctgtcgttcc attgaaagca
     7861 ttggcagaga aatacaagtt cttgatattt gaggacagaa aattcgccga tatcggtaac
     7921 acagtcaaat tacaatatac atcgggcgtt taccgtatcg cagaatggtc tgatatcacc
     7981 aacgcccacg gggttactgg tgctggtatt gttgctggct tgaaacaagg tgcgcaagag
     8041 gtcaccaaag aaccaagggg attattgatg cttgctgaat tgtcttccaa gggttctcta
     8101 gcacacggtg aatatactaa gggtaccgtt gatattgcaa agagtgataa agatttcgtt
     8161 attgggttca ttgctcagaa cgatatggga ggaagagaag aagggtttga ttggctaatc
     8221 atgaccccag gtgtaggttt agacgacaaa ggcgatgcat tgggtcagca gtacagaacc
     8281 gtcgacgaag ttgtaagtgg tggatcagat atcatcattg ttggcagagg acttttcgcc
     8341 aagggtagag atcctaaggt tgaaggtgaa agatacagaa atgctggatg ggaagcgtac
     8401 caaaagagaa tcagcgctcc ccattaatta tacaggaaac ttaatagaac aaatcacata
     8461 tttaatctaa tagccacctg cattggcacg gtgcaacact acttcaactt catcttacaa
     8521 aaagatcacg tgatctgttg tattgggatc tctagaccta ataacttcgt atagcataca
     8581 ttatacgaag ttatattaag ggttgctcga ccagaaagcg accacccaac tttggctgat
     8641 aatagcgtat aaacaatgca tactttgtac gttcaaaata caatgcagta gatatattta
     8701 tgcatattac atataataca tatcacatag gaagcaacag gcgcgttgga cttttaattt
     8761 tcgaggaccg cgaatcctta catcacaccc aatcccccac aagtgatccc
//
