LOCUS       sequence-327156-        8840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and 
            SKP1-fusing rice auxin receptor gene OsTIR1. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
  TITLE     Auxin-mediated protein depletion for metabolic engineering in 
            terpene-producing yeast.
  JOURNAL   Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.
  PUBMED    33594068
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-16"; volume: "12(1):1051. doi"; pages: " 
            10.1038/s41467-021-21313-1"
COMMENT     Sequence Label: pJAID8Z105
FEATURES             Location/Qualifiers
     source          1..8840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             431..1141
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1148..1279
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             1472..1492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1502..1772
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     protein_bind    2472..2483
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             complement(2869..2886)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             2898..4625
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(4861..4877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4861..4877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4874..4896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4885..4901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4909..4915,4916..4933,4934..4939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4954..4975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5092..5109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5263..5851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5343..5362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6022..6813,6814..6882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6645..6664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6883..6987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7055..7073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7111..7133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7233..7252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7446..7468
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7460..7477
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7461..7477
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8591..8624)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(8835..8840,1..408)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
       61 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
      121 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac
      181 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
      241 cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      301 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      361 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
      421 aatgggatcc tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
      481 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
      541 ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc
      601 aaccttagtc actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat
      661 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
      721 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
      781 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
      841 tcacaaattg gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa
      901 gaatggtatc aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt
      961 agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa
     1021 ccattactta tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat
     1081 ggtcttgtta gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa
     1141 aagatctcca aaagacccag ctaaacctcc agccaaggcc caagtcgtcg gctggccacc
     1201 agttaggtct tacagaaaga acgtaatggt tagttgtcaa aagtcctccg gtggtccaga
     1261 agctgctgcc tttgttaaga tgttcagcga attaattaac ttccaaaatg aaggtcatga
     1321 gtgccaatgc caatgtggta gctgcaaaaa taatgaacaa tgccaaaaat catgtagctg
     1381 cccaacgggg tgtaacagcg acgacaaatg cccctgcggt aacaagtctg aagaaaccaa
     1441 gaagtcatgc tgctctggga aatgtacccc acctaagaag aaaagaaagg tttaaactag
     1501 tattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta
     1561 cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac gtatatatag
     1621 actattattt atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt
     1681 ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgttcgg gttcagcgta
     1741 ttttaagttt aataactcga aaattctgcg ttcgttaaag ctttcgagaa ggatattatt
     1801 tgcggccgcg attgcaagaa ttgatctccc tcatcgatgt cataaatcag taaatataaa
     1861 cattaaatac attacacgta gccacccgct gtatagcata aaactaactt aaaattattc
     1921 atatgcgttt cccggggccg aagcgttatt gccgatattc ggtagccgat tccgctggct
     1981 tttttttttt gaacgaggca aaaaagtttc gtgactgaac ttcttcgtca ctggttttgt
     2041 tgataatttt tatcgttttt tacttcgaga ttgaaaacag tgttctgaat tttagcaacg
     2101 atatcgcacc gcacgtttag cctgatcaaa ccttatatat acctctacct taaaatatat
     2161 atatatatag tattcgaatt ccgaattgtg tgtctccgcg gttagtgatt gttatacaca
     2221 aacagaatac aggaaagtaa atcaatacaa taataaaagc tagcaaaatg gtgacttcta
     2281 atgttgtcct agtgagtggt gagggtgaac gattcaccgt agacaagaaa atcgcggaga
     2341 gatccctgct attgaaaaac tatctgaacg atatgcatga cagcaaccta caaaacaact
     2401 cagattcaga atcagattca gactcggaaa cgaaccacaa aagcaaagac aacaataacg
     2461 gtgacgacga cgatgaggat gacgacgaga ttgtgatgcc cgtgcccaat gttcggtctt
     2521 ccgttttgca gaaggttatc gaatgggccg agcaccacag agactctaac ttccctgacg
     2581 aagatgacga cgattccaga aagagtgctc ccgtggactc atgggacaga gaattcctga
     2641 aagtggacca agagatgcta tacgaaatta ttctggcggc aaactacctc aacatcaagc
     2701 ccttgcttga cgctggctgc aaggtggttg ctgaaatgat caggggaagg tctcccgagg
     2761 aaattagaag aacgtttaat atcgtcaacg acttcactcc agaggaggag gctgccatta
     2821 ggcgtgaaaa tgaatgggct gaagaccgtg gtatctttga tttgggtgct ggtgctggtg
     2881 ctggtgacct tacttctatg acctatttcc cagaagaagt cgttgaacac atcttctctt
     2941 tcttgccagc tcaaagagat agaaatactg tttccttggt ttgtaaggtt tggtatgaaa
     3001 tcgaaagatt gagtagacgt ggtgttttcg tcggtaattg ttacgctgtt agagctggta
     3061 gagttgctgc tagattccca aatgttagag ccttgactgt caaaggtaaa ccacattttg
     3121 ctgactttaa cttggtccca ccagattggg gtggttatgc tggtccatgg attgaagctg
     3181 ccgctagagg ttgtcacggt ttggaagaat tgcgtatgaa aagaatggtc gtttctgacg
     3241 aatctttgga attattggcc cgttcttttc caagattcag agctttggtt ttgatctctt
     3301 gtgaaggttt cagtactgac ggtttggctg ccgttgcttc ccattgtaaa ttattgagag
     3361 aattggactt gcaagaaaat gaagtcgaag atagaggtcc aagatggtta tcttgttttc
     3421 cagactcttg tacttctttg gtctctttga acttcgcttg tattaagggt gaagtcaacg
     3481 ctggttcttt agaaagattg gtctcccgtt ctccaaactt acgttctttg agattgaatc
     3541 gttctgtttc cgttgatacc ttggctaaaa tcttattgag aaccccaaac ttggaagact
     3601 taggtactgg taacttgacc gacgatttcc aaactgaatc ctactttaaa ttaacttctg
     3661 ctttagaaaa gtgtaagatg ttgagatcct tatctggttt ttgggacgcc tctccagtct
     3721 gtttatcttt tatctatcca ttgtgtgctc aattaactgg tttaaactta tcttacgctc
     3781 caaccttgga tgctagtgac ttaaccaaga tgattagtag atgtgttaag ttacaaagat
     3841 tatgggtttt agattgtatt tccgataagg gtttacaagt cgttgctagt agctgtaagg
     3901 atttgcaaga attaagagtc tttccaagtg atttttacgt tgctggttac agcgctgtta
     3961 ctgaagaagg tttggtcgct gtttctttgg gttgtccaaa gttgaacagc ttattgtact
     4021 tttgtcatca aatgactaat gctgccttag tcactgttgc taagaactgt ccaaacttca
     4081 cccgtttcag attgtgtatt ttggaaccag gtaaaccaga cgttgtcacc tcccaaccat
     4141 tggatgaagg tttcggtgct atcgttagag aatgtaaggg tttgcaaaga ttatctatct
     4201 ctggtttgtt aactgacaaa gttttcatgt acatcggtaa atatgccaaa caattagaaa
     4261 tgttgagcat tgctttcgct ggtgactctg acaagggtat gatgcacgtt atgaatggtt
     4321 gtaagaattt gagaaagttg gaaattagag attctccatt cggtgacgct gccttattgg
     4381 gtaattttgc tagatacgaa actatgagaa gtttgtggat gagttcctgt aacgttacct
     4441 tgaaaggttg tcaagtctta gccagcaaga tgccaatgtt gaacgtcgaa gtcatcaacg
     4501 aaagagacgg tagtaacgaa atggaagaaa atcacggtga cttaccaaag gtagaaaagt
     4561 tgtacgtata cagaactact gctggtgcta gagatgatgc tccaaatttc gtaaagattt
     4621 tgtaactcga gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac
     4681 agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta
     4741 ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag
     4801 ttattacccg ggaatctcgg tcgtaatgat ttaaatgcat gcaagcttgg cgtaatcatg
     4861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7021 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7081 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     7141 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     7201 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     7261 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7321 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7381 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7441 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcatttaaa tttttttgat
     7501 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     7561 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     7621 agaacaaaaa cctgcaggaa acgaagataa aacatgtcca caaaatcata taccagtaga
     7681 gctgagactc atgcaagtcc ggttgcatcg aaacttttac gtttaatgga tgaaaagaag
     7741 accaatttgt gtgcttctct tgacgttcgt tcgactgatg agctattgaa acttgttgaa
     7801 acgttgggtc catacatttg ccttttgaaa acacacgttg atatcttgga tgatttcagt
     7861 tatgagggta ctgtcgttcc attgaaagca ttggcagaga aatacaagtt cttgatattt
     7921 gaggacagaa aattcgccga tatcggtaac acagtcaaat tacaatatac atcgggcgtt
     7981 taccgtatcg cagaatggtc tgatatcacc aacgcccacg gggttactgg tgctggtatt
     8041 gttgctggct tgaaacaagg tgcgcaagag gtcaccaaag aaccaagggg attattgatg
     8101 cttgctgaat tgtcttccaa gggttctcta gcacacggtg aatatactaa gggtaccgtt
     8161 gatattgcaa agagtgataa agatttcgtt attgggttca ttgctcagaa cgatatggga
     8221 ggaagagaag aagggtttga ttggctaatc atgaccccag gtgtaggttt agacgacaaa
     8281 ggcgatgcat tgggtcagca gtacagaacc gtcgacgaag ttgtaagtgg tggatcagat
     8341 atcatcattg ttggcagagg acttttcgcc aagggtagag atcctaaggt tgaaggtgaa
     8401 agatacagaa atgctggatg ggaagcgtac caaaagagaa tcagcgctcc ccattaatta
     8461 tacaggaaac ttaatagaac aaatcacata tttaatctaa tagccacctg cattggcacg
     8521 gtgcaacact acttcaactt catcttacaa aaagatcacg tgatctgttg tattgggatc
     8581 tctagaccta ataacttcgt atagcataca ttatacgaag ttatattaag ggttgctcga
     8641 ccagaaagcg accacccaac tttggctgat aatagcgtat aaacaatgca tactttgtac
     8701 gttcaaaata caatgcagta gatatattta tgcatattac atataataca tatcacatag
     8761 gaagcaacag gcgcgttgga cttttaattt tcgaggaccg cgaatcctta catcacaccc
     8821 aatcccccac aagtgatccc
//
