LOCUS       sequence-327162-        5734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnma1b gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnma1b
FEATURES             Location/Qualifiers
     source          1..5734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..71
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     63..80
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     64..80
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    96..195
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3386..3485)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3502..3521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3503..3521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3526..3542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3526..3542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3644..3663)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3655..4464
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3722..3741)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4611..5199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5100..5119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5353..5370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5529..5556)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5648..5734)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
       61 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
      121 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
      181 acaaaaaagc aggctccgcg gccgccccct tcaccatgac ctctgtcaaa gactgggctg
      241 gagtcatgat ctctgcacag acgctcaccg gacgcgtgct ggtggtgttg gtgtttgccc
      301 tcagcatcgg cgctctggtg atttacttca tcgactcctc tgatcccatt gaatcctgtg
      361 agcatttcga caagaacttc accctgcaga tcgacatggc cttcaacgtc ttcttcttgc
      421 tgtattttgg actgcgtttt atagcagcca atgataagct gtggttctgg ctggaggtaa
      481 actcagtggt ggatttcttc acggttccgc cggtctttgt gtctgtttac ctgaaccgga
      541 gctggctggg actgaggttc ttaagagcct tgaggctgat ccagttttca gaaatattgc
      601 agtttttaaa tatcttaaaa acaagcaact ccatcaagtt ggtgaatttg tgctccatct
      661 ttataagcac atggctgacg gcggctggat tcattcattt ggtggagaac tctggagatc
      721 cttgggagaa cttccagaat tcgcagcctc tgtcgtactg ggagtgtgtg tatttactga
      781 tggtgaccat gtccacagtg ggctatggag atgtgtgtgc gaaaacaacc ctcggccgcc
      841 tcttcatggt cttcttcatt ctgggaggac tggccatgtt tgccagctac gttcctgaaa
      901 tcatagagtt aataggaaac cgcaagaaat atggtggttc ctatagtgca gtaaatggga
      961 gaaagcatat cgtggtgtgt ggacacatca cactggagag cgtctccaac ttcctcaaag
     1021 acttcctaca caaggacagg gatgatgtca atgtagaaat agtttttctc cataatattt
     1081 cccctaatct tgagctggaa gccttattca agagacattt cacccaggtg gagttttacc
     1141 agggctctgt gctcaatcct cacgatctcg ctcgagtcaa gattgaatca gccgatgcct
     1201 gtctgatctt ggccaataaa tactgcgcag atcctgatgc agaggacgca tccaatatca
     1261 tgagggtgat atccattaaa aactatcatc caaagatacg catcattaca cagatgctgc
     1321 agtaccacaa taaggctcat ctgctgaaca tcccgagctg gaactggaag gaaggagacg
     1381 atgccatctg cctggcggag ctaaagttgg gcttcatcgc ccagagctgc ctggcacagg
     1441 ggctctccac catgctggcc aacctgttct caatgaggtc ctatatcaag attgaggagg
     1501 acacgtggca gaagtattat ctagaaggag tagccaatga gatgtacact gagtatctgt
     1561 ccagtgcctt cgtgggttta tctttcccca ccgtctgtga actctgctat gtgaagctga
     1621 agctgctgct gatcgccatc gagtataaat cagaccagag agagagcagc actctgatta
     1681 accctggcaa tcatgtgaag atgcaggagg gaacgctggg ctttttcatc gccagcgacg
     1741 ccaaagaggt caagagggct cttttctact gtaaagcctg tcatgatgac attacagatc
     1801 ctaaacgcat caagaagtgc ggatgcaaac ggaacaagag tttcggctac aagagaatgc
     1861 gtctggcatg ttgtgtagct tgcggtgggg cagaaagtgg ctgctcgtgc atgtcagaca
     1921 gtgtggctaa taacaaggag gtgttacaac gctcctctaa aagtaactat aacggataca
     1981 tcaaatcaat agtggaggag gagcagcact cttctctctc ccccaagaag aagcagcgta
     2041 atggaggaat gaggacatcc cccacctgct cccccaaaaa cctcatcaga cacgatccgc
     2101 tgctggagat ggagaccatg gatgagaaca tcaagaagta tgattccaca gggatgtttc
     2161 actggtgtcc atctaaggac atcgagaggg tcctcctgac gcgcagtgag gcggcgatga
     2221 cggtgttgag tggtcatgtg gtggtctgta ttttcggaga tgtgaagtct gctctgattg
     2281 gcctgaggaa cctggtgatg ccgctgagag ccagcaactt ccactaccat gagctgaagc
     2341 ccatcgtgtt cgtgggctca ctggagtacc tgaagagaga gtgggaaaca ctgcacaact
     2401 tccccaaagt ctccatcctg cccggaactc ctctgagtcg ggctgatctg agagcagtga
     2461 acattaatct gtgtgatatg tgtgtaatcc tctccgccaa ccagaacaac atcgacgacg
     2521 cgtctctgca ggataaggag tgtattctgg cgtcactcaa catcaagtcc atgcagtttg
     2581 acgacagcat cggcctcctg caggccaact ctcaaggctt cactcctcca ggaatggatc
     2641 gctcgtctcc agaaaacagt cctgttcacg ggttggtgcg gcaggcgtcg ataaccacag
     2701 gagcaaacat tcccatcatc actgaactcg tgaacgactc gaatgtgcag tttctggatc
     2761 aggatgatga tgatgatccc gacactgagc tgtatctcac tcaacccttc gcctgcggga
     2821 ccgccttcgc cgtcagcgtg ctggactcat tgatgagtgc tacgtacttc aatgataata
     2881 tcctgacgct gatccgcacg ctggtgacgg gtggagcgac gccggagctg gagggtctgc
     2941 tggcggagga gaacgctctg cgtggaggat acagcacacc acagaccctc gccaatcgag
     3001 accgctgccg agtcgcccag ctggcgcttt atgacggacc cttcgccgac ctcggggacg
     3061 gtggctgtta cggtgattta ttctgtaaag cgttgaagac gtataacatg ctgtgtttcg
     3121 gcatctaccg gctgagagac gctcatctga ccgcagcgag cctctgcacc aaacgctacg
     3181 tgatcacgaa cccgccgtat gagtttgatc tggttcccac agatctgatc ttctgtctga
     3241 tgcagtttga tcataacgcg ggtcagagtc gcagtaatct aaacactcag agcagcttct
     3301 gtcctgtgcg cagagcatcc cgcagccgct gcaccccccg cgacaagcag aagtacgtcc
     3361 ctctgtagaa agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
     3421 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     3481 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     3541 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     3601 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     3661 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     3721 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     3781 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     3841 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     3901 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     3961 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     4021 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     4081 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     4141 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     4201 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     4261 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     4321 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     4381 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     4441 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     4501 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     4561 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4741 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5401 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5461 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     5521 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     5581 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     5641 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     5701 cccagtcttc cgactgagcc tttcgtttta tttg
//
