LOCUS       sequence-327163-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite Gateway (attR4-attR3) vector for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
  TITLE     Combinatorial assembly of clone libraries using site-specific 
            recombination.
  JOURNAL   Methods Mol Biol. 2014;1116:193-208. doi: 
            10.1007/978-1-62703-764-8_14.
  PUBMED    24395366
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1116:193-208. doi"; pages: " 
            10.1007/978-1-62703-764-8_14"
COMMENT     Sequence Label: pDest-669 (attR4-attR3)
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4123..4246
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4287..4592)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4389..4408)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4912..5592)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     5507..5526
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(5646..5652,5653..5670,5671..5676))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5701..5824)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5843..6431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5896..5916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6314..6325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6487..6986
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6522..6545)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6919..6938
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7004..8029
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7004..8029
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             8403..8636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8708..8829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8745..8764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8799..8818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8869..9004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9024..9043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9025..9043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9053..9070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9053..9069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9062..9084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9211..9666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9298..9317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9508..9529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag atcaactttg tatagaaaag
     4141 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     4201 aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac
     4261 tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt
     4321 ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc
     4381 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg
     4441 taaagttcac gggggacttt atctgacagc agacgtgcac tggccagggg gatcaccatc
     4501 cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc
     4561 tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcggcaaa
     4621 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
     4681 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccgaaccgga
     4741 gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta
     4801 atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata
     4861 ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc
     4921 cggatcctct agattacgcc ccgcctgcca ctcatcgcag tactgttgta attcattaag
     4981 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     5041 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     5101 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     5161 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     5221 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     5281 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     5341 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     5401 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     5461 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     5521 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     5581 ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct aactcaaaat
     5641 ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgcggccgc
     5701 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     5761 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt attatacata
     5821 gttgatgtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5881 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5941 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6001 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6061 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6121 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6181 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6241 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6301 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6361 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6421 cgcctccccg cctggaattc tgcagatatc cggactagtg atctctcgag gttaacgaat
     6481 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6541 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6601 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6661 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6721 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6781 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6841 gaaggggtgg gtccgggggc gggctcaggg gnnggctcag gggcggggcg ggcgcccgaa
     6901 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6961 cctcttcctc atctccgggc ctttcgacct gcatcccgcc accatgaaaa agcctgaact
     7021 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     7081 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     7141 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     7201 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     7261 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     7321 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     7381 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     7441 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     7501 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     7561 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     7621 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     7681 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     7741 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     7801 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     7861 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     7921 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     7981 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg
     8041 accgaacaag agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg
     8101 caaatgcgag agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc
     8161 tcgctcggct gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt
     8221 ggtccccagg gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat
     8281 tggagatcgc cgcccgtgcc tgccgattgg gtgcagatcc gtcgagggcc cggtaccttt
     8341 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8401 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     8461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8641 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8701 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8821 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8881 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8941 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9001 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     9061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9181 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     9241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9421 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9541 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9601 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     9661 caatttaggt ggcacttttc ggggaaatgt g
//
