LOCUS       sequence-327164-        3361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnmb2b gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnmb2b
FEATURES             Location/Qualifiers
     source          1..3361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(40..126)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     175..197
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     189..206
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     190..206
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    222..321
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1139..1238)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1255..1274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1256..1274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1279..1295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1279..1295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1397..1416)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1408..2217
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1475..1494)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2364..2952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2853..2872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3106..3123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3282..3309)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
       61 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
      121 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
      181 cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt
      241 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
      301 ctttgtacaa aaaagcaggc tccgcggccg cccccttcac catgtttctg tgggctggaa
      361 gcaaagcagc tcaaggatca ggaagtgaga gaaggacgat ttatcagaag attcgagagt
      421 atgatatttt ggagaagagg aggacggtga ccgcgctgaa ggctggagag gacagagcca
      481 ttctcttagg cctcagcatg atcctatgct ccgccatgat gtactttgtg ttgggcatca
      541 caatggtgcg ttcttactca gacagtgttt ggacagagga gtcaaactgc acagtgttga
      601 actcgagtgt agttgctgag attaactgta gctacagctg tggttcagag tgccggaaaa
      661 gctccaggta tccctgtctt caagtgtatg tcagtttgga ctctggtaaa gttcagcggc
      721 tctcatacaa cgaggaagcg catgaggcca atcctgagtg tttttacatc ccaaagtgcc
      781 gaaaggagca aacactaatg cacaccatag tgatgaacat ctcggagcgg ctcaagatgc
      841 accagcaggt tctgtgttat tatgacccct cagagcagca ggacggcgta cttctgacac
      901 gtctctacgg ccgcggagcg atcatcagct cgctcctttg gcccacatgc acccttgtcg
      961 ggggaacgct tatcatcgca atggtgaagc tcactcaata cctgtccttc ctgtgcgagc
     1021 agatcagccg cattaaaagg atggggcttt actccttaag aggagcttct catgctcagg
     1081 tctgcacccg gaagtttaac ggcggcatcc aggatgtggt ttaagggtgg gcgcgccgac
     1141 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1201 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1261 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1321 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1381 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1441 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1501 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1561 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1621 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1681 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1741 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1801 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1861 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1921 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1981 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2041 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2101 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2161 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2221 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2281 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3001 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3061 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3121 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3241 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3301 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3361 c
//
