LOCUS       sequence-327165-        3453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnmb3 gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnmb3
FEATURES             Location/Qualifiers
     source          1..3453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..62)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(154..240)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     289..311
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     303..320
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     304..320
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    336..435
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1345..1444)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1461..1480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1462..1480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1485..1501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1485..1501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1603..1622)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1614..2423
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1681..1700)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2570..3158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3059..3078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3312..3329
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
       61 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
      121 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
      181 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
      241 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
      301 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
      361 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
      421 acaaaaaagc aggctccgcg gccgccccct tcaccatgct gctgaatcag tcccctcggg
      481 ggtctttcag tgttcctgtt aacatcaccc tgcagggtgc gcggcggcga cacatgcggg
      541 atgtaatcca ccatcaggcc cagctgcatg attctggggg gaactggagg aaagagaggg
      601 agctgaagtg caacgaggaa gcccgggctc aaatcccaga ctccagtgtc ggagaagaca
      661 gagccgtcct cctgggcttc ctcatgatga cctgctccat cctcatgtac ctgatggtgg
      721 gaatcttaat ggtcaaaccg tgcatccata gtgattggat aagttctgtg aactgctcac
      781 tggtgcacat tgatttgtcg gatgaagtga tggactgccg tggcattgtt aacttcaaat
      841 gcctgcgtgc tttagtaaac atcacaacct ctaacaaaac gtcatcgctt caactttact
      901 atgatgaaga cgctgtgaat tccaggctta agacaaactc tttctcccag tgtttctaca
      961 ctcccaaatg ccaacgaaac aagacggaac aagaaaccga ggcccgtaac atcaaacaag
     1021 cattaattac acaaagggaa aacctgacat gctttctcag cccttcacac ccagaagacg
     1081 ccatctgcag gagaaaatat aacttagaaa tggccctgta ttacttgctg tggcccagcc
     1141 tcatgttgtt tggcgggatc gtcctggtgg gactagtgaa gctcaaccag catctggctt
     1201 tgctctgctc tgagatcagc agggatgagt ttctggacaa tcagagtaac caggcagagg
     1261 gaaggatcta ccagctcttg aaatgcagat ctggagagac cgcagagcag cataaaccac
     1321 ttagctagaa gggtgggcgc gccgacccag ctttcttgta caaagttggc attataagaa
     1381 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1441 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1501 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     1561 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     1621 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     1681 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     1741 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     1801 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     1861 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     1921 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     1981 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2041 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2101 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2161 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2221 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2281 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2341 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2401 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2461 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     2521 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2701 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3421 aagcgggcag tgagcgcaac gcaattaata cgc
//
