LOCUS       sequence-327167-        4215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnn1b gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnn1b
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..154
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     146..163
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     147..163
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    179..278
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1950..2049)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2066..2085)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2067..2085)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2090..2106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2090..2106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2208..2227)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2219..3028
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2286..2305)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3175..3763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3664..3683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3917..3934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4093..4120)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(4212..4215,1..83))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
       61 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
      121 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca
      181 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
      241 cttttttata atgccaactt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
      301 gaacagctgc aagtacaatg ggggcatagt cagaccactc ggcagtctgg gggcgtcccg
      361 gaggaacctc gcagagctgg actctgagac tcagcctttg cagaccctgc acacgtccgg
      421 cctggaggtg gtggtctcca aaggaaacgg ggaagaccct agtaagcagt ctagtgagaa
      481 cctggtgacg cagagaccgt gcagcgcgcc acataagaag aacaaggaca ttggctataa
      541 actcggccac cggagggcgc tgttcgagaa gcgcaagcgc cttagtgact acgccctgat
      601 ttttgggatg tttgggattg ttgtcatggt gacggagact gaactctcct ggggagttta
      661 tacaaaggaa tcctcatatt catttgcact gaaatgcctt attagcctct ccactgtaat
      721 tctactctgt ctgatcatta tgtaccacgc aagagagatc cagctcttta tggtggacaa
      781 tggcgcagac gactggcgga ttgccatgac gtacgagcgg atcctcttca tagtcctgga
      841 gctgctggtg tgcgccatcc accccatccc aggccagtat gtgttcacat ggacggcccg
      901 cctggccttc acctacacgc cgtcggtggc agacgcagac gtggacatca tcctgtctat
      961 ccccatgttc ctgcgcctct acctgatcgg ccgtgtaatg ctcctgcaca gtaaactctt
     1021 tacagacgcc tcgtcgcgca gcatcggagc cctcaataag atcaacttca acacgcgctt
     1081 cgtcatgaag accctcatga ccatctgccc aggcaccgtc ctgctggtct tcagcatctc
     1141 gtcatggatc atcgccgcct ggactgtgca tgtctgcgag agatatcacg ataaagtgga
     1201 agtcaccagt aacttcctgg gagccatgtg gctgatctcc ataaccttcc tgtcgatcgg
     1261 gtatggagac atggtgccta acacatactg tgggaaggga gtgtgtctcc tcacaggaat
     1321 catgggggcc gggtgcactg ccctcgtggt ggctgtcgta gccagaaaac tagagctcac
     1381 caaggctgaa aagcatgtcc ataactttat gatggacact caactctgca aaagagtgaa
     1441 aaacacggct gctaatgtac tcagggaaac atggctaatc tacaaacaca caaaacttgt
     1501 gaagaagatt gaccatgcca aggtccgcaa gcatcagcgc aagttcctgc aggccattca
     1561 ccaacttcga agtgtgaaga tggagcaaag gaaactgaat gaccaagcaa acacgctggt
     1621 ggacttagca aagacacaga atgtgatgta cgacctggta tcagaactgc aggagcgcag
     1681 cgaggagctg gaaaagcgaa tcggtcacct cgaaacaaaa ctggactcca taaacagcag
     1741 tctgcaggct ctgcctggcc tgctctcaca agccatacag cagcaacagc accacctgct
     1801 ggacgggctg acccagcgcg gattcaccct cacgagaccc tcctcgcaga cctccgaacg
     1861 ctcctgggcg tcctccgtgc accgacggag atcaccctct accgcaccac acacgtcctc
     1921 cgatagcggc tagaagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat
     1981 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2041 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2101 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2161 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2221 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2281 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     2341 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2401 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2461 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     2521 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2581 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2641 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2701 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2761 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2821 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2881 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2941 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3001 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3061 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3121 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3961 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4021 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     4081 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     4141 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     4201 gctcccggcg gattt
//
