LOCUS       sequence-327168-        4026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnn4 gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnn4
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(76..103)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(195..281)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     330..352
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     344..361
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     345..361
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    377..476
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1959..2058)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2075..2094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2076..2094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2099..2115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2099..2115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2217..2236)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2228..3037
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2295..2314)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3184..3772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3673..3692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3926..3943
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
       61 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
      121 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
      181 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      241 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
      301 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
      361 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
      421 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccgc
      481 ggccgccccc ttcaccatgg agatgaccgc tcgcttcgcg cagttggaga ccatggtgga
      541 cggcaaaaac tgcgatggcc ttgtgaacca taacggggaa acaacagaga taagagttcc
      601 gctgtcccac atggggaaga gaatgagtcc tattgaaggg ttgaacctct acagactgcg
      661 cgacagaaag ttcttactgg aggacaaaag gcgcctgtgc gcgtgggctc tgggcaccgc
      721 gctgttgggc attctgctaa tggtgttaca cgctgagctg tgcccagtag tgtaccagcc
      781 gggatccata tatgccctct ccataaacgg tctcattagt ttctccactg gttgtctgct
      841 cattctcatc atcgccttcc attataaaga catcagactt ttcatcattg accacaacca
      901 ggtggactgg cgcattgcca tgaccagcta ccgggtgctt ggcatctccc tggagctcat
      961 ggtctgcgcc atccatcccg tggtcatcta cctgaggcca gacggggagg acaaacttgg
     1021 gaggaaacca gagcagaatt cgtcaacccc cctgtgcttg tcatccagtc acagagaagt
     1081 catgctggac gttgagctgc tgctgtcggc cctcatgttc ttgagactgt atctggtgca
     1141 cagagccgtc ctgttgcaca gtaaagtgct cctcagcgcc tcgtacagga gcatcggatc
     1201 cctcaacaac atcaacttca ccttccgctt cgtgctaaag gtgttaatga acaaatatcc
     1261 agcccgaacg ctgctggtct tcattatgtt cttctggctc tctgcatctt ggatgctgcg
     1321 actgtgtgaa agggagaagc aggtagacac aggtggcatg gacacagcgc tgtggctcat
     1381 tgccatcacc ttcctcacag tgggctatgg agacgtgtcc cctcacacca gctgtggaaa
     1441 ggtggtgtgt ctcttcacag gcgtcatggg tgtggcttgt actgctatgt tggtagcagt
     1501 catgactaag aaattggcac ttaacaaagg agagaaacat gtgcatttct tcatgatgga
     1561 cattcagatc tcaaagcgga tcagacatgc agcagccaat gtgctgaggg aatgttggct
     1621 tctgcatcgc actacccaaa ctaaatacac tagtggagaa catcgacacc atcagaggtg
     1681 cctgctggag gccatccgtg tatttcgtca tttgcggttg aagcagagga agttaagaga
     1741 ctatgccagt gaaatggtgg atctctcaaa gatgcagatg atcatgtgtg atctgagtgc
     1801 aaactggaac agctcttatc acgagctgga gcagcgaatc atctccatgg agcagaagct
     1861 ggatgaactg agacgctcct tccaaaacac ctcggagctg ctcacacagg cgctacatca
     1921 ccgcagactg gactacaggt gaaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1981 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2041 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2101 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2161 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2221 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2281 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2341 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2401 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2461 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2521 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2581 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2641 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     2701 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     2761 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     2821 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     2881 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     2941 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3001 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3061 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3121 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3841 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3901 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4021 gtttcc
//
