LOCUS       sequence-327171-        2735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human TERF2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2735)
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-77:TERF2-mEGFP
FEATURES             Location/Qualifiers
     source          1..2735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aactggatct ttgcaggttc cagactagtg gggctggtag gacaatcttt gaagctagac
       61 cagttactgg gcctctcagg aagaggcacc cacaaccacc atcctgaact aaggtctgag
      121 ctggaggttc tccatttggt caagtgccag ttggtgaatt ccaagagtat cagagatatc
      181 tgaaagtgac tcagaccctc tatgggacta tgggtggtcc ttttgcttat ttttctaatt
      241 gttctacaac aaaatgtgtt atttttgtga ttacaaagag tatatgtcta gcggctgggt
      301 tgccaggtgc attctattta ttggtatcac actggtcttt gctttataat tacagtattt
      361 gttaaaatac aaatttgttt aaagactttc ctctgtgtat gctctcttcc acaattttca
      421 aagtgtttaa taaggtgcac atatttggtt ttcttttttt tcttttcttt tttttgagac
      481 ggagtttcgc tcttgttgcc cagactggag tgcaatggcg cgatctcggc tcaccgcaac
      541 ctccgcctcg cgggttcaag cgagtctcct gcctcagcct cctgagtagc taggattaca
      601 ggcatgcgcc accacgtctg gctaattttg tatttttagt agagatgggg tttctccatg
      661 ttggtcaggc tggtctcgaa ctcccgacct caggtgatcc acccgcctca gcctcccaaa
      721 gtgctgggat tacaggcgta gagccaccga tcccggcctg tttttcagtc tctaatacac
      781 tgttccgcgt ccctagcata gtagctgttt tctgtaaatt cggagcgaag ttgactgcaa
      841 aacccgccgg gttcaccggc ctgggcggtg tggacttcaa gccccaggag gcattgcggc
      901 cggcacatcg ggaactacgg cgtctgagaa gggggccggg ccctccggtc ctttccctcc
      961 cagaagcccg cgggcggctc ggaacgctgt ttctatcatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc agaacccaga tccccgcagc
     1741 gggagcaggg acggcgggcc ccgcttccgg cccgggcgtc gtgcgtgacc cagcggcgtc
     1801 acagccgagg aagcggcccg gccgggaggg cggggagggc gcgcggcgat cggacacgat
     1861 ggcgggagga ggcgggagta gcgacggcag cgggcgggca gctggcaggc gggcgtcccg
     1921 cagtagcggg cgggcccggc gggggcgcca cgagccgggg ctggggggcc cggcggagcg
     1981 cggcgcgggg gaggcacggc tggaagaggc agtcaatcgc tgggtgctca agttctactt
     2041 ccacgaggcg ctgcgggcct ttcggggtag ccggtacggg gacttcagac agatccggga
     2101 catcatgcag ggtgagggcc gggccggggg aggggggttg gagcgccggg gaagggggtc
     2161 cgggggaccg ggagtcggga ctgggggtcg cccagccaac gatgtcttct ctcccagctt
     2221 tgcttgtcag gcccttgggg aaggagcaca ccgtgtcccg attgctgcgg gttatgcagt
     2281 gtctgtcgcg gattgaagaa ggggaaaatt taggtatggc tatttttgtg ttcttctctg
     2341 ggcttacttg cgtagggttt tgttttaaaa cttatatcta gaactgaagg attccaggtt
     2401 aaagtgcgat agtttccatc agagtggtct ttttcattct acgatgggtc tgttttctgg
     2461 ctcgactggc tctagttttc tccggttaag gttgtcttgg ctgcttttct gtgttttttt
     2521 taaatcttct cccacctgtt aattgctcac cccaactttg ctcctgtcta aagtgagtta
     2581 tgttcatatg gacatctttt ttcccccatg gtagagagtt tttcagagtg gaagtactct
     2641 gacatttttc ccctctgaat ttaacttgaa agctatttag ctgttgtcag tttcttgcca
     2701 ctcgtatctg aggaggatgg gtatattctg aattt
//
