LOCUS       sequence-327182-        6030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnt1a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pDONR221-kcnt1a
FEATURES             Location/Qualifiers
     source          1..6030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(135..162)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(254..340)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     389..411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     403..420
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     404..420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    436..535
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4022..4121)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4138..4157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4139..4157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4162..4178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4162..4178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4280..4299)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4291..5100
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4358..4377)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5247..5835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5736..5755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5989..6006
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
      121 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      181 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      241 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      301 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      361 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
      421 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      481 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggcttcacc
      541 atgaccactg gaacatctgt ggttccttcc cttcaaacct atttccggtt ccgagacctg
      601 ttactaggag accagacatt ttatgatgac agggttcatg tggagtttta tgtggacgag
      661 aacacattta aagagagact taaacttttt ttcatcaaaa accagagatc cagtttaagg
      721 atccgaatat tcaacttctg cctgaagctt ttgacatgtg ttctctacat catacgggtg
      781 atgacggaca atccagccca gcttcgccac acatgtgtga actgcagctc acagaacgac
      841 atcaactggg agctgatatt ctgggtggac agaaaggtac cggtgtgggc cattcaggtg
      901 attgtagcat ccatcagttt tatggaggcc atgttactga cctacctgag ctacaaggga
      961 aatttatggg agcagatctt tcaggtgtct ttcctgctgg agatgctcaa caccgtcccg
     1021 ttcatcatca cgatcttctg gcctcctctc agaaacctct tcatcccagt gttcctcaac
     1081 tgctggctgg ccaaatgtgc tctggagagc atgataaatg atctgcacag agccattcag
     1141 aggactcact ccgccatgtt taatcaggtc ctgatcctca tctgcaccct cctgtgtctc
     1201 gtctttaccg gcacgtgtgg gatccagcat ttggagcggg ccggtaagaa gagtctgtcg
     1261 ctcttcaacg ctctgtattt ctgcatcgtg accttctcaa cggtgggttt cggggacgta
     1321 acgccccaga tctggccttc tcagctgctg gtggtggtga tgatctgcgt ggccctcgtc
     1381 gtgctgccgc ttcagtttga ggagttgatt tatctgtgga tggaaagaca gaaatctgga
     1441 ggaaactaca gcaggcatcg cgctcagaca gagagacacg tggttttatg tgtcagtact
     1501 ctgaaaatcg acctgctgat ggactttctc aatgagttct acgctcaccc ccacacacag
     1561 gattattatg tggtcatctt gtgtccgtgt gaagtggacg ttcaggtcag gaggatttta
     1621 cagattcctc tctggtcaca gagggtcatt tatcttcaag gctccgccct caaaaaccag
     1681 gatttgctga gagccaagat ggacgatgcg gaggcatgtt ttattcttag cagcaggaat
     1741 gaagcggatc gcatggcagc agatcaccag accattctga gggcttgggc tgtgaaagac
     1801 ttcgctccaa actgccctct gtatgtccag atccttaaac ccgaaaacaa attccatgtt
     1861 aaatttgcag atcacgttgt gtgtgaggaa gagtttaaat acgccatgtt ggctctgaac
     1921 tgtctgtgtc cggccacttc tactctgatc actttactgg ttcacacatc tcgcggacaa
     1981 gagggccagc agtctccgga gcagtggcag aggatgtatg gtcgatgttc gggtaatgag
     2041 gtctatcata tccgcctggg tgacagtatg ttcttcaggg agtacaacag caaaagcttt
     2101 acctatgctg cctttcacgc tcacaaaaag tacggtgtgt gtatgattgg tgtgaagaga
     2161 gaagataata agagcatctt gttgaaccct ggaccgcgac acatcatgtc tgccagcgac
     2221 acctgttact acatcaacat cacgaaagag gaaaactccg ccttcatctt cagacaggag
     2281 gaagaccagg gaaaggggcg gagccactgc gactttttaa acagcccgtc aggactacct
     2341 gttcatagca ttattgctag catgggaacg gttgccatgg attttcagaa caccagtccc
     2401 actgagagcg gactcaaact ggctccgccc acagagaacg aactgggaag tcgacgacca
     2461 agtatagcgc ccgttctgga gattgctgac tccgcctcct tactgccgtg tgacctgctg
     2521 agcgaccaat cagaggacga ggccggacac tcgaatgaag atattacagc cactgatttt
     2581 gtgaaaggtt atcccccgaa ctctccatat attgggagct ctccgaccct ttgccatctt
     2641 ttacctcaga aagccccttt ctgctgttta cggcttgacc agagctgtga gcatgtttcc
     2701 tttgaagacg caaaagcata cggcttcaaa aataaattaa ttatcgtttc tgcggagacg
     2761 gctggaaacg gcctttacaa cttcatagtg cctttaagag cctattacag gcccagaaga
     2821 gaactcaacc ctatagtcct gctgttagac aacctacccg atgaccactt ccttgaggcc
     2881 atatcctgct tcccaatggt gtattatatg ggtggcacca ttgacaactt ggacagccta
     2941 ttgcagtgtg gcgttctgta tgctgataat ctagtggttg ttgacaaaga aagcacgatg
     3001 agtgcagagg aagactatat ggctgacgct aaaaccatcg tcaatgtgca aaccatgttc
     3061 aggttgtttc cgagtctcag tataatcaca gaactgactc acccgtccaa catgagattc
     3121 atgcagttca gagcgaagga ctgctactcg ctcgccctgt ccaaactaga gaagaaggaa
     3181 cgtgacaaag gctcaaatct ggccttcatg tttcgccttc cctttgctgc tggccgagtc
     3241 ttcagtatca gcatgctgga cacactcctg taccagtgtt ttgtgaagga ctacatgatc
     3301 cccatagcca gattactgct gggattggac accacacctg gatctggata cctgtgtgct
     3361 atgcacatat gtgaggctga tctctggatc cgcacatacg ggagactctt ccagaagttc
     3421 tgctcatcca gttcagagat tcccatcgga atctaccgca cagaatcaca cgcaccacct
     3481 gagcttcaaa gctcagtcag catagacggt ttaaacgaca ctagagagaa aggcgaggag
     3541 tcaaagatcc tcacccggag ctcctcaagc agcgaccaat cagatcaccc actgctgaga
     3601 aagaagagca tgcattggac aagacgtctc agtaggcgga gtgtaaagcg gagcgactcc
     3661 tccctcagct ccatccagca gcgtctgggt gtgttacggc gctctgagcg ggaggagctc
     3721 actgaactgg tcagaaaccg catgcagcat ttaggcctgc acactggcgg atacaatgtc
     3781 acaaatttaa cagccagtga tgtgatgaat cgggttaatt tgggctattt gcaagatgag
     3841 ttgaatgacc agcagaactc catctcatat gtgctcataa accccgcccc cgacacactg
     3901 ctgcagctca atgatgtcgt gtttttgatt cgtccggatc cactggctca cgtacctgaa
     3961 gctccgcctg gacagaagcg ccgctgccgc tccaccacag acacaccaga actcaactag
     4021 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     4081 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     4141 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     4201 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     4261 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     4321 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     4381 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     4441 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     4501 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     4561 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     4621 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     4681 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     4741 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     4801 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     4861 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4921 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4981 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     5041 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     5101 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     5161 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6001 agcgaggaag cggaagagcg cccaatacgc
//
