LOCUS       sequence-327188-        6048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnt2a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnt2b
FEATURES             Location/Qualifiers
     source          1..6048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1952..2051)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2067..2084)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2067..2083)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2076..2098)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2147..2233
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2325..2352
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2511..2528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2682..3270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2762..2781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3417..4226)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4140..4159
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4218..4237
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4339..4355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4339..4355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4360..4379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4360..4378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4396..4495
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcctcagatt ggtcccccag caggtcaaac gtggagttgt gtacaccgtt caccacttcc
       61 aggacagggg cgatgctgtg tcttctttcc tcaccggagc cactgacggc aggcagcgat
      121 agcgtgttac aaacgctcac ttctgtcctg cagccacttc tgctcaccgt cccactgccc
      181 agagctgccg gccccgagcc ttcaggactg ctgtcacttg agtcctgtag gtcgatcgcc
      241 accgtgccca tgctggctat gatgctgtgc actggcaggc gagtgaggcc atgatagatg
      301 gtctgtggga cccccctgga atcacccccg gggcctcgca ccggctctct gtggccacgg
      361 acaaatgctg aattttcctc tttggagatg ttgatgtaga aacaggtgtc agaggctccc
      421 ataatgtgac aaggtccagg gttcaggagg atattggcgg tgtccatcct gcgcacccca
      481 atcagacaca caccgtattt cttatgtgca tgaaaggagg caaaggtgaa actctttccc
      541 tgatattctc caaaaaactt gctctcctcc agtcggatgt ggtgcacctc attggctgcg
      601 cagcgccggt aggtgcgctg ccattgatcg actgagctgg caccaccttc cctgttgagg
      661 ggctggaagg ctcccgctcg ctgcctgaag gcttctgaag atgctgtttg tcctctagag
      721 gagtgaatca acagtgtgat cagggtagag gtggcaggac acacacagtt cagggccaac
      781 atagcatatt taaactcttc ctcacaaacc acatgatctg cgaacttgat gtgaaactta
      841 ttctcaggtt tgaggatctg cacataaagt gggcagtttg gagcaaagtc tttcacggcc
      901 caggccctaa gaatggtctg atggtctttg ccctcattag gtcctgatct ttgagtgcag
      961 acccctgcag gtaaatgacc ctctgtgccc acagcgggat atgaagaacc ctccgcacct
     1021 ggacgtccat ctcagcagga cacaagatga ccacatagta atcctgcagt ctggggtgag
     1081 caaagaactc gttgaggaag tccatgagca ggtctatctt gagacagctg acacataaca
     1141 ctacatgctt ctcagtctga gcacggtaac ggctgtagtt tcctccagac ttctgtcgct
     1201 ccatccatag gaaagccaac tgctcaaact gtattggcag aactataaga gccacacaga
     1261 tcatgatgac caccagcaac tgggacggcc agatctgagg tgtgacgtcc ccaaagccca
     1321 ccgtagagaa agtgacaaca cagaaataca gagagtcaaa gagtgtcagg ttgttcccgg
     1381 ctcgttccaa gtgctgaata ccacaaatac aggtgaagat gaggcagagg agggtgctga
     1441 tgaggatgag cacctggttg aacatggctg aatgtgtgcg ctgaatcgct ctgtgcaggt
     1501 cattagtgat cacaaaaggg acggcactga tcatctccag aatgaaggga atgcgcaaga
     1561 cttgttccca aacgttgccc ttgtagctta ggtaaaccag caacatggtc tctgaaaaac
     1621 tgatgaatgc aacaagcacc tgaagagccc agagtggcag aggcctgttc acccaaataa
     1681 tgagggacca gtcaaaatgg cttctgttgt gggcagaggc attctgtctg agacagccag
     1741 gacaatctct ttcctcccga gggtcatcca gcaggactcg tacaatgtac agcaagcagc
     1801 tcagaacctt cagaaagaag ttgaagagtc gcactctcaa acctgtcgtc cgtctgccag
     1861 tccccgagca acaggtctcg gaatcggtac cgcggtggca gcggcagcac ctccttctcc
     1921 agctccacca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa agttggcatt
     1981 ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2041 atcattattt ggggcccgag cttaagactg gccgtcgttt tacaacgtcg tgactgggaa
     2101 aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     2161 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     2221 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg
     2281 gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac
     2341 ggatggcctt tttgcgtttc tacaaactct tcctggctag cggtacgcgt attaattgcg
     2401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3301 gacgctcagt ggaacgacgc gtaactcacg ttaagggatt ttggtcatga gcttgcgccg
     3361 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
     3421 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3481 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     3541 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     3601 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     3661 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     3721 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     3781 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     3841 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     3901 tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca
     3961 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     4021 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     4081 tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta
     4141 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     4201 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     4261 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     4321 gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc actatagggg
     4381 atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     4441 caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtcggcg
     4501 cgcccaccct ttcataggtg agtgctatcc tgtgtgtcga atctgtgtcc ttctgctggc
     4561 cctgcgctgg gtttgcgact gccggcctgg ttaggcactg gtgacagaag atcaggtctg
     4621 atcagataca cgacatcatg aagctccagg cgtgtgtcag gggaagggtt gataagaata
     4681 taggacagtc tgttgccctg gtctgtcatc ccattgaatc cagatggtga gagccccagg
     4741 tgcttcatgc gtgtgcggac caaagcattg agctcttgcc tctcggagcg gcgaaacagg
     4801 ctgagcctct gctgggtgat gcgctcagct gcgctcccca ctcccttcgc cccgccggat
     4861 cctcgtccac tcttacgact tagtcgtcgt gcccactgca tgctcttcct cctcagtaga
     4921 gggtggtcca tggcttgtga ggatgaggag gaggagggcg gctctgatga cgttgagttg
     4981 cgatgaagac cggagcggca gagcagaggc tcacggggct cgcgtgtatc ctcatagtct
     5041 tccaccgtga tggacacctg agactctgag acgggtagtt tatgtgcttc tgtgcggtag
     5101 atgccgatgg gaatgtcacc agtcgatgag cacagctttt ggtagagacg accataagta
     5161 cgaatccaca aatcctcctc tgtaatcttc atagcacaga ggaaaccaga tccaggcatg
     5221 gagtctagac ctagcagcag tctggtaatg gagatcatgt agtccttcac aaaagactga
     5281 taaagcagag tgtctaacat actgacacta aacacctttc cagccgcaaa cggtaatctg
     5341 aacatgaaca ctaggttgga gcccctctct ctctctttct tctccagttt ggaaagagct
     5401 agagaatagt ggtctttcac tttgaactgc atgaatcgca tgttggccgg atgtgtgagt
     5461 tcggttataa tactgagagc tggaaaaagc ctgaagagtg tctgaacatt aacaatggtc
     5521 ttggcatcag ccatgtagtc ctcctctgca atcatagagc tctctttgtc cacaactacc
     5581 atggtgtcag caaatgtcac accacaacgc aaaagactat ccagattgtc aatggagccc
     5641 actgtgtaaa agaccatagg aaaccagcag atggcctcca agaagtctgc ctcaggtacg
     5701 ttctccaata acaaaacaat ggggttaagc tccttcctgg ggcggtaata tgctcgcaga
     5761 gggacgatga agttataaag gccatttcca gcactttccg ctgatacgat gatcagtttg
     5821 tttttgaagc cataagagcg agcatcttca tagggcatgt gatgacaagc tttatccaga
     5881 cgcagacagc aaaatggcat tttctcctgc agaaggtggc agagggctgg agagctgccg
     5941 atgtatggag agtttagtgg gtagccttta acccactcgc agtgtgctcc ccaccactgg
     6001 cctttctgct ctcctccatc ctcctccttg ccctcctctc ctccctcg
//
