LOCUS       sequence-327211-        4421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp14
FEATURES             Location/Qualifiers
     source          1..4421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2896..2995)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3012..3031)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3013..3031)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3036..3052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3036..3052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3483..4274
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4367..4421,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctctcaga ttgtaactgg cctttttaaa
     1321 gattgctcta gagaaacttc tggcctctca cctgcttatg caccaacata tgttagtgtt
     1381 gatgacaagt ataagacgag tgatgagctt tgcgtgaatc ttaatttacc cgcaaatgtc
     1441 ccatactctc gtgttatttc caggatgggc tttaaactcg atgcaacagt tcctggatat
     1501 cctaagcttt tcattactcg tgaagaggct gtaaggcaag ttcgaagctg gataggcttc
     1561 gatgttgagg gtgctcatgc ttcccgtaat gcatgtggca ccaatgtgcc tctacaatta
     1621 ggattttcaa ctggtgtgaa ctttgttgtt cagccagttg gtgttgtaga cactgagtgg
     1681 ggtaacatgt taacgggcat tgctgcacgt cctccaccag gtgaacagtt taagcacctc
     1741 gtgcctctta tgcataaggg ggctgcgtgg cctattgtta gacgacgtat agtgcaaatg
     1801 ttgtcagaca ctttagacaa attgtctgat tactgtacgt ttgtttgttg ggctcatggc
     1861 tttgaattaa cgtctgcatc atacttttgc aagataggta aggaacagaa gtgttgcatg
     1921 tgcaatagac gcgctgcagc gtactcttca cctctgcaat cttatgcctg ctggactcat
     1981 tcctgcggtt atgattatgt ctacaaccct ttctttgtcg atgttcaaca gtggggttat
     2041 gtaggcaatc ttgctactaa tcacgatcgt tattgctctg tccatcaagg agctcatgtg
     2101 gcttctaatg atgcaataat gactcgttgt ttagctattc attcttgttt tatagaacgt
     2161 gtggattggg atatagagta tccttatatc tcacatgaaa agaaattgaa ttcctgttgt
     2221 agaatcgttg agcgcaacgt cgtacgtgct gctcttcttg ccggttcatt tgacaaagtc
     2281 tatgatattg gcaatcctaa aggaattcct attgttgatg accctgtggt tgattggcat
     2341 tattttgatg cacagccctt gaccaggaag gtacaacagc ttttctatac agaggacatg
     2401 gcctcaagat ttgctgatgg gctctgctta ttttggaact gtaatgtacc aaaatatcct
     2461 aataatgcaa ttgtatgcag gtttgacaca cgtgtgcatt ctgagttcaa tttgccaggt
     2521 tgtgatggcg gtagtttgta tgttaacaag cacgcttttc atacaccagc atatgatgtg
     2581 agtgcattcc gtgatctgaa acctttacca ttcttttatt attctactac accatgtgaa
     2641 gtgcatggta atggtagtat gatagaggat attgattatg tacccctaaa atctgcagtc
     2701 tgtattacag cttgtaattt agggggcgct gtttgtagga agcatgctac agagtacaga
     2761 gagtatatgg aagcatataa tcttgtctct gcatcaggtt tccgcctttg gtgttataag
     2821 acctttgata tttataatct ctggtctact tttacaaaag ttcaacttaa ttaagacgcg
     2881 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     2941 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     3001 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     3061 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     3121 tcctctagac cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
     3181 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     3241 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     3301 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     3361 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     3421 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3481 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3541 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3601 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3661 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3721 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3781 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3841 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3901 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3961 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     4021 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     4081 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     4141 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4201 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     4261 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     4321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc g
//
