LOCUS       sequence-327255-        4349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp4
FEATURES             Location/Qualifiers
     source          1..4349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2824..2923)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2940..2959)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2941..2959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2964..2980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2964..2980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3411..4202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4295..4349,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccaagattg ttagtacttg ttttaaactt
     1321 atgcttaagg ccacattatt gtgcgttctt gctgcattgg tttgttatat cgttatgcca
     1381 gtacatacat tgtcaatcca tgatggttac acaaatgaaa tcattggtta caaagccatt
     1441 caggatggtg tcactcgtga catcatttct actgatgatt gttttgcaaa taaacatgct
     1501 ggttttgacg catggtttag ccagcgtggt ggttcataca aaaatgacaa aagctgccct
     1561 gtagtagctg ctatcattac aagagagatt ggtttcatag tgcctggctt accgggtact
     1621 gtgctgagag caatcaatgg tgacttcttg cattttctac ctcgtgtttt tagtgctgtt
     1681 ggcaacattt gctacacacc ttccaaactc attgagtata gtgattttgc tacctctgct
     1741 tgcgttcttg ctgctgagtg tacaattttt aaggatgcta tgggcaaacc tgtgccatat
     1801 tgttatgaca ctaatttgct agagggttct atttcttata gtgagcttcg tccagacact
     1861 cgttatgtgc ttatggatgg ttccatcata cagtttccta acacttacct ggagggttct
     1921 gttagagtag taacaacttt tgatgctgag tactgtagac atggtacatg cgaaaggtca
     1981 gaagtaggta tttgcctatc taccagtggt agatgggttc ttaataatga gcattacaga
     2041 gctctatcag gagttttctg tggtgttgat gcgatgaatc tcatagctaa catctttact
     2101 cctcttgtgc aacctgtggg tgctttagat gtgtctgctt cagtagtggc tggtggtatt
     2161 attgccatat tggtgacttg tgctgcctac tactttatga aattcagacg tgtttttggt
     2221 gagtacaacc atgttgttgc tgctaatgca cttttgtttt tgatgtcttt cactatactc
     2281 tgtctggtac cagcttacag ctttctgccg ggagtctact cagtctttta cttgtacttg
     2341 acattctatt tcaccaatga tgtttcattc ttggctcacc ttcaatggtt tgccatgttt
     2401 tctcctattg tgcctttttg gataacagca atctatgtat tctgtatttc tctgaagcac
     2461 tgccattggt tctttaacaa ctatcttagg aaaagagtca tgtttaatgg agttacattt
     2521 agtaccttcg aggaggctgc tttgtgtacc tttttgctca acaaggaaat gtacctaaaa
     2581 ttgcgtagcg agacactgtt gccacttaca cagtataaca ggtatcttgc tctatataac
     2641 aagtacaagt atttcagtgg agccttagat actaccagct atcgtgaagc agcttgctgc
     2701 cacttagcaa aggctctaaa tgactttagc aactcaggtg ctgatgttct ctaccaacca
     2761 ccacagacat caatcacttc tgctgttctg cagcttaatt aagacgcgat cgcggcctca
     2821 tgggcccagc tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt
     2881 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2941 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3001 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     3061 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
     3121 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
     3421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     4021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     4081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     4141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4201 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4321 tctgctgctt gcaaacaaaa aaaccaccg
//
