LOCUS       sequence-32738-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T/A Cloning Vector for dsRNA generation and the generation of sense 
            and anti-sense probes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, 
            Sweedler JV, Newmark PA
  TITLE     Genome-wide analyses reveal a role for peptide hormones in planarian
            germline development.
  JOURNAL   PLoS Biol. 2010  . 8(10):e1000509.
  PUBMED    20967238
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010  . 8(10):e1000509."
COMMENT     Sequence Label: pJC53.2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..344)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(141..160)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
       61 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      121 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      181 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
      241 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
      301 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
      361 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
      421 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
      481 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
      541 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
      601 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
      661 cttttagtaa gccggatcca cgcggcgttt acgccccccc tgccactcat cgcagtactg
      721 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      781 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
      841 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
      901 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat a
//
