LOCUS       sequence-32746-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Cui Z, Geurts AM, Liu G, Kaufman CD, Hackett PB
  TITLE     Structure-function analysis of the inverted terminal repeats of the 
            sleeping beauty transposon.
  JOURNAL   J Mol Biol. 2002 May 17. 318(5):1221-35.
  PUBMED    12083513
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2002 May 17. 318(5):1221-35."
COMMENT     Sequence Label: pT2/SVNeo
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(418..438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        423..752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      603..738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(873..892)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca gttgaagtcg
       61 gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta ctccacaaat
      121 ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt gtgcatgaca
      181 caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat tcactgtatc
      241 acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt aaacagcttg
      301 gaaaattcca gaaaatgatg tcatggcttt agaagcttcc cacaataagt tgggtgaatt
      361 ttggctcatt cctcctttct ataggattga ggtcagagct ttgtgatgga attctgtgga
      421 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      481 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      541 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      601 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      661 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
      721 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgg atatccattt
      781 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
      841 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      901 ca
//
