LOCUS       sequence-32747-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     Transposon cloning vectors
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT/BH
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(681..701)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(681..699)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(720..736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(733..755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    744..760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(768..774,775..792,793..798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    813..834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttgagtcgga agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact
       61 ccacaaattt cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt
      121 gcatgacaca agtcattttt ccaacaattg tttacagaca gattatttca cttataattc
      181 actgtatcac aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa
      241 acagcttgga aaattccaga aaatgatgtc atggctttag aagctagatc tagagcggcc
      301 gcactagtga attcgatatc aagcttgtgg aaggctactc gaaatgtttg acccaagtta
      361 aacaatttaa aggcaatgct accaaatact aattgagtgt atgttaactt ctgacccact
      421 gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt ctctctacta ttattctgat
      481 atttcacatt cttaaaataa agtggtgatc ctaactgacc ttaagacagg gaatctttac
      541 tcggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc taaggtgtat
      601 gtaaacttcc gacttcaact gtaggggatc ctctagctag agtcgacctc gagggggggc
      661 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
      721 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      781 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      841 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      901 ggccaacgcg cggggagagg cggtt
//
