LOCUS       sequence-32748-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Transposon cloning vectors
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT/HB
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(686..706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(738..760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    749..765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(773..779,780..797,798..803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    818..839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact cgtttttcaa
       61 ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta ggacatctac
      121 tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta tttcacttat
      181 aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt tgactgtgcc
      241 tttaaacagc ttggaaaatt ccagaaaatg atgtcatggc tttagaagct tgatatcgaa
      301 ttcactagtg cggccgctct agatctagct tgtggaaggc tactcgaaat gtttgaccca
      361 agttaaacaa tttaaaggca atgctaccaa atactaattg agtgtatgtt aacttctgac
      421 ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc tactattatt
      481 ctgatatttc acattcttaa aataaagtgg tgatcctaac tgaccttaag acagggaatc
      541 tttactcgga ttaaatgtca ggaattgtga aaaagtgagt ttaaatgtat ttggctaagg
      601 tgtatgtaaa cttccgactt caactgtagg ggatcctcta gctagagtcg acctcgaggg
      661 ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      841 ttgcgttgcg ctcactgccc gctttccagt cgggaaa
//
