LOCUS       sequence-32750-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     Transposon cloning vectors
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT2/HB
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(694..714)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(694..712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(733..749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(746..768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    757..773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(781..787,788..805,806..811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    826..847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agtcggaagt ttacatacac ttaagttgga gtcattaaaa ctcgtttttc aactactcca
       61 caaatttctt gttaacaaac aatagttttg gcaagtcagt taggacatct actttgtgca
      121 tgacacaagt catttttcca acaattgttt acagacagat tatttcactt ataattcact
      181 gtatcacaat tccagtgggt cagaagttta catacactaa gttgactgtg cctttaaaca
      241 gcttggaaaa ttccagaaaa tgatgtcatg gctttagaag cttgatatcc atggaattca
      301 ctagtgcgcg cggccgctct agatggccag atctagcttg tggaaggcta ctcgaaatgt
      361 ttgacccaag ttaaacaatt taaaggcaat gctaccaaat actaattgag tgtatgtaaa
      421 cttctgaccc actgggaatg tgatgaaaga aataaaagct gaaatgaatc attctctcta
      481 ctattattct gatatttcac attcttaaaa taaagtggtg atcctaactg acctaagaca
      541 gggaattttt actaggatta aatgtcagga attgtgaaaa agtgagttta aatgtatttg
      601 gctaaggtgt atgtaaactt ccgacttcaa ctgtataggg atcctctagc tagagtcgac
      661 ctcgaggggg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      721 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      781 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      841 cacattaatt gcgttgcgct cactgcccgc tttcc
//
