LOCUS       sequence-327520-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the DiB2‐split protein fused with blue fluorescent 
            protein TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Bozhanova NG, Gavrikov AS, Mishin AS, Meiler J
  TITLE     DiB-splits: nature-guided design of a novel fluorescent labeling 
            split system.
  JOURNAL   Sci Rep. 2020 Jul 6;10(1):11049. doi: 10.1038/s41598-020-67095-2.
  PUBMED    32632329
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-07-6"; volume: "10(1):11049. doi"; pages: " 
            10.1038/s41598-020-67095-2"
COMMENT     Sequence Label: TagBFP-DiB2-splitN1–125
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..752
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatgagcg
       61 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
      121 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      181 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
      241 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
      301 ccttccctga gggcttcaca tgggagagag tcaccacata cgaggacggg ggcgtgctga
      361 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      421 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
      481 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
      541 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
      601 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
      661 aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
      721 acctccctag caaactgggg cacaagctta atgatccccc ggtcgccaca atgg
//
