LOCUS       sequence-327531-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-fragment of the DiB2‐split protein fused with blue fluorescent 
            protein TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Bozhanova NG, Gavrikov AS, Mishin AS, Meiler J
  TITLE     DiB-splits: nature-guided design of a novel fluorescent labeling 
            split system.
  JOURNAL   Sci Rep. 2020 Jul 6;10(1):11049. doi: 10.1038/s41598-020-67095-2.
  PUBMED    32632329
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-07-6"; volume: "10(1):11049. doi"; pages: " 
            10.1038/s41598-020-67095-2"
COMMENT     Sequence Label: TagBFP-DiB2-splitC110–177
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..753
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatgagc
       61 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
      121 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
      181 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
      241 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
      301 tccttccctg agggcttcac atgggagaga gtcaccacat acgaggacgg gggcgtgctg
      361 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
      421 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
      481 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
      541 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
      601 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
      661 aaggaggcca acaacgaaac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
      721 gacctcccta gcaaactggg gcacaagctt aatgatcccc cggtcgccac aatgggg
//
