LOCUS       sequence-327550-        3991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains elements for building reporter plasmid for IBM. SrpR 
            reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ho TYH, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L, Dalchau N,
            Wang B
  TITLE     A systematic approach to inserting split inteins for Boolean logic 
            gate engineering and basal activity reduction.
  JOURNAL   Nat Commun. 2021 Apr 13;12(1):2200. doi: 10.1038/s41467-021-22404-9.
  PUBMED    33850130
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-13"; volume: "12(1):2200. doi"; pages: " 
            10.1038/s41467-021-22404-9"
COMMENT     Sequence Label: IBMc330
FEATURES             Location/Qualifiers
     source          1..3991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(140..226)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     241..263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             397..408
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1208..1302
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1383..1474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1475..1543,1544..2335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2339..2378
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2525..2633
                     /note="incP origin of transfer"
     CDS             complement(2644..3594)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFDIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3642..3864
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
       61 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gcggcgcgcc
      121 cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctttaattaa
      241 agcggataac aatttcacac aggaggccgc ctaggccgcg gccgcgcgaa ttccttctag
      301 aggaagacag tacgagagga ttcgttacca attgacagct agctcagtcc taggtatata
      361 catacatgct tgtttgtttg taaactacta gagattaaag aggagaaata ctagatggtg
      421 agtaaaggag aagctgtgat taaagagttc atgcgcttca aagttcacat ggagggttct
      481 atgaacggtc acgagttcga gatcgaaggc gaaggcgagg gccgtccgta tgaaggcacc
      541 cagaccgcca aactgaaagt gactaaaggc ggcccgctgc ctttttcctg ggacatcctg
      601 agcccgcaat ttatgtacgg ttctagggcg ttcatcaaac acccagcgga tatcccggac
      661 tattataagc agtcttttcc ggaaggtttc aagtgggaac gcgtaatgaa ttttgaagat
      721 ggtggtgccg tgaccgtcac tcaggacacc tccctggagg atggcaccct gatctataaa
      781 gttaaactgc gtggtactaa ttttccacct gatggcccgg tgatgcagaa aaagacgatg
      841 ggttgggagg cgtctaccga acgcttgtat ccggaagatg gtgtgctgaa aggcgacatt
      901 aaaatggccc tgcgcctgaa agatggcggc cgctatctgg ctgacttcaa aaccacgtac
      961 aaagccaaga aacctgtgca gatgcctggc gcgtacaatg tggaccgcaa actggacatc
     1021 acctctcata atgaagatta tacggtggta gagcaatatg agcgctccga gggtcgtcat
     1081 tctaccggtg gcatggatga actatacaaa taataactcg gtaccaaatt ccagaaaaga
     1141 gacgctttcg agcgtctttt ttcgttttgg tccgtacagc tgcgtcttct actagtactg
     1201 cagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     1261 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
     1321 tacgatttac gtatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
     1381 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1441 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
     1501 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
     1561 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
     1621 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
     1681 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
     1741 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
     1801 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
     1861 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
     1921 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
     1981 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     2041 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     2101 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     2161 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     2221 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     2281 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     2341 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     2401 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     2461 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     2521 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     2581 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     2641 gcctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
     2701 gagccatgag aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc
     2761 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
     2821 gttatgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
     2881 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
     2941 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
     3001 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
     3061 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
     3121 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
     3181 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
     3241 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     3301 tgtccactgg aaaatatcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     3361 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     3421 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     3481 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     3541 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     3601 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     3661 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     3721 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     3781 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     3841 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     3901 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     3961 caccctcgca agctcgggca aatcgctgaa t
//
