LOCUS       sequence-327553-        3760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express miR-125a~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir125~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..3760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1166..1247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1258..1275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1429..2017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1509..1528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2188..2979,2980..3048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2811..2830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3049..3153)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3186..3267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagggatc
      541 ctctagtcag ctgacgcgtg ctagcgcggc cgccgttttt ggtctttctg tctctggctc
      601 tcagaatgtc tctgtgccta tctccatctc tgacccccac cccagggtct accgggccac
      661 cgcacaccat gttgccagtc tctaggtccc tgagaccctt taacctgtga ggacatccag
      721 ggtcacaggt gaggttcttg ggagcctggc gtctggccca accacacacc tggggaattg
      781 ctggcctgac ttctgacccc tgactcctca tacccttcct ccagagcatg acatttgacc
      841 accaactgaa acctgacaag aagctctctg ctcagcctgt cacaacctac tgactgccag
      901 ggcacttggg aatggcaagg aaaccgttac cattactgag tttagtaatg gtaatggttc
      961 tcttgctata cccagaaaac gtgccaggaa gagaactcag gaccctgaag cagactactg
     1021 gaagggagac tccaaagctt gtcgacgata tctccagagg atcataatca gccataccac
     1081 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1141 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcccc gagcttcctc
     1261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     2821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3181 cgtcggcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3241 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3301 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     3361 gctgattatg atcctctaga ctgcagggct gtcggccgcg gcggcccccg tggtgtggag
     3421 tgcccctcag tgcggcgaga gaggtcccgg cctgagatac atacttcttt acattccata
     3481 gcttagcagg tccatatggg catataaaga agtatgtttc ccaagcaggc agtctgcatg
     3541 cagttccaag accccgggac gccgccccgg tagaagctga ccccagatct gggcccccgc
     3601 ggcatatgac cggtgaattc tccaggcgat ctgacggttc actaaacgag ctctgcttat
     3661 ataggcctcc caccgtacac gccacctcga catactcgag tagttattaa tagtaatcaa
     3721 ttacggggtc attagttcat agcccatata tggagttccg
//
