LOCUS       sequence-327578-        8701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, 
            Blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8701)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-TRE-PtenWT-Blast
FEATURES             Location/Qualifiers
     source          1..8701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1159..1747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1648..1667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1901..1918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2035..2056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2071..2076,2077..2094,2095..2101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2109..2125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2114..2136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2133..2149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2133..2149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2168..2188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2170..2188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2216..2442
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2443..2623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2670..2795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3288..3521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3706..3750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4017..4133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4149..4527
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4157..4175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4193..4211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4229..4247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4265..4283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4301..4319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4337..4355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4436..4460
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5766..6354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5819..5839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6237..6248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6368..6867
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6403..6426)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6800..6819
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6896..7294
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7438..7671
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7743..7864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7780..7799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7834..7853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7904..8039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8059..8078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8060..8078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8088..8105)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8088..8104)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8097..8119)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8246..8674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8333..8352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8543..8564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2041 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     2101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2161 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2221 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2281 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     2341 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2401 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2641 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2701 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2761 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2821 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2881 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2941 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3001 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3061 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3121 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3181 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3241 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3301 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3361 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3421 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3481 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3541 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3601 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3661 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3721 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3781 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3841 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3901 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3961 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     4021 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4081 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatt
     4141 ctagattcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     4201 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     4261 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     4321 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     4381 agtgatagag aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc
     4441 gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact
     4501 ttccgtacca cttcctaccc tcgtaaagtc gagcttgcgt tggatccatg acagccatca
     4561 tcaaagagat cgttagcaga aacaaaagga gatatcaaga ggatggattc gacttagact
     4621 tgacctatat ttatccaaat attattgcta tgggatttcc tgcagaaaga cttgaaggtg
     4681 tatacaggaa caatattgat gatgtagtaa ggtttttgga ttcaaagcat aaaaaccatt
     4741 acaagatata caatctatgt gctgagagac attatgacac cgccaaattt aactgcagag
     4801 ttgcacagta tccttttgaa gaccataacc caccacagct agaacttatc aaacccttct
     4861 gtgaagatct tgaccaatgg ctaagtgaag atgacaatca tgttgcagca attcactgta
     4921 aagctggaaa gggacggact ggtgtaatga tttgtgcata tttattgcat cggggcaaat
     4981 ttttaaaggc acaagaggcc ctagattttt atggggaagt aaggaccaga gacaaaaagg
     5041 gagtcacaat tcccagtcag aggcgctatg tatattatta tagctacctg ctaaaaaatc
     5101 acctggatta cagacccgtg gcactgctgt ttcacaagat gatgtttgaa actattccaa
     5161 tgttcagtgg cggaacttgc aatcctcagt ttgtggtctg ccagctaaag gtgaagatat
     5221 attcctccaa ttcaggaccc acgcggcggg aggacaagtt catgtacttt gagttccctc
     5281 agccattgcc tgtgtgtggt gatatcaaag tagagttctt ccacaaacag aacaagatgc
     5341 tcaaaaagga caaaatgttt cacttttggg taaatacgtt cttcatacca ggaccagagg
     5401 aaacctcaga aaaagtggaa aatggaagtc tttgtgatca ggaaatcgat agcatttgca
     5461 gtatagagcg tgcagataat gacaaggagt atcttgtact caccctaaca aaaaacgatc
     5521 ttgacaaagc aaacaaagac aaggccaacc gatacttctc tccaaatttt aaggtgaaac
     5581 tatactttac aaaaacagta gaggagccat caaatccaga ggctagcagt tcaacttctg
     5641 tgactccaga tgttagtgac aatgaacctg atcattatag atattctgac accactgact
     5701 ctgatccaga gaatgaacct tttgatgaag atcagcattc acaaattaca aaagtctaag
     5761 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6361 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     6421 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     6481 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     6541 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     6601 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     6661 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     6721 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     6781 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     6841 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccgct agcccatggc
     6901 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     6961 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     7021 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     7081 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     7141 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     7201 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     7261 attgctgccc tctggttatg tgtgggaggg ctaagctagc cgcccgcccc acgacccgca
     7321 gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac
     7381 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7441 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7501 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7561 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7621 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7681 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7741 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7861 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7921 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7981 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8041 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     8101 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8161 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8221 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8281 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8341 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8401 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8461 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8521 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8581 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8641 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8701 t
//
