LOCUS       sequence-327586-        1596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast high copy plasmid with dCas9, sgRNA expression cassette and 
            VPR activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: EC2_10_dCas9_VPR_HC_sgRNA
FEATURES             Location/Qualifiers
     source          1..1596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             28..177
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             202..222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             652..1008
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1027..1596
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 cccaagaaaa agcgcaaggt aggatccgac gcattggacg attttgatct ggatatgctg
       61 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
      121 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
      181 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
      241 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
      301 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
      361 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
      421 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
      481 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
      541 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
      601 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
      661 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
      721 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
      781 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
      841 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
      901 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
      961 gacgaggact tcagctctat cgccgacatg gacttctccg cactgctggg tagcggatcg
     1021 ggatctcggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     1081 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     1141 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     1201 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     1261 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agaaacgagc
     1321 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     1381 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     1441 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     1501 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     1561 agcacaggac tgtccatctt cgacacatct ctgttt
//
