LOCUS       sequence-327602-        8413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression (EF1a promoter) of GFP cDNA plus 
            miRE-embedded shRenilla (control shRNA), Blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8413)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-EF1a-GFP-miRE_shRen-Blast
FEATURES             Location/Qualifiers
     source          1..8413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1159..1747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1648..1667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1901..1918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2035..2056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2071..2076,2077..2094,2095..2101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2109..2125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2114..2136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2133..2149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2133..2149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2168..2188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2170..2188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2216..2442
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2443..2623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2670..2795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3288..3521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3706..3750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4017..4133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4140..4351
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4371..4380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4377..4379,4380..4382,4383..5096)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4377..4379,4380..4382,4383..5093)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4422..4443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4683..4702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5030..5051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           5290..5304
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    5480..6068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5533..5553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5951..5962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6081..6580
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6116..6139)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6513..6532
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6609..7007
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7150..7383
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7455..7576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7492..7511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7546..7565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7616..7751
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7771..7790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7772..7790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7800..7817)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7800..7816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7809..7831)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7958..8386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8045..8064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8255..8276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2041 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     2101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2161 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2221 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2281 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     2341 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2401 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2641 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2701 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2761 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2821 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2881 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2941 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3001 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3061 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3121 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3181 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3241 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3301 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3361 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3421 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3481 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3541 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3601 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3661 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3721 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3781 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3841 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3901 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3961 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     4021 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4081 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatg
     4141 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
     4201 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4261 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4321 ttttcgcaac gggtttgccg ccagaacaca gtctagagga tccaccggtc gccaccatgg
     4381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4441 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaatgac
     5101 gtacggcggc cgcctcgact agggataaca gggtaattgt ttgaatgagg cttcagtact
     5161 ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc gacttcttaa
     5221 cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgcagga attataatgc
     5281 ttatctatag tgaagccaca gatgtataga taagcattat aattcctatg cctactgcct
     5341 cggacttcaa ggggctagaa ttcgagcaat tatcttgttt actaaaactg aataccttgc
     5401 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
     5461 tcaattgacg cgtgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5881 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6061 ctccccgcct ggattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     6121 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     6181 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     6241 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     6301 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     6361 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     6421 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     6481 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     6541 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     6601 gctagcccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     6661 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     6721 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     6781 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     6841 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     6901 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     6961 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagct agccgcccgc
     7021 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcattc gagggcccgg
     7081 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7141 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     7201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7381 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7441 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7561 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7621 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7681 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7741 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7801 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7861 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7921 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7981 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8041 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8101 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8161 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8221 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8281 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8341 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8401 acgcttacaa ttt
//
