LOCUS       sequence-327616-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 RBD in bacterial system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Ahmad J, Jiang J, Boyd LF, Natarajan K, Margulies DH
  TITLE     Synthetic nanobody-SARS-CoV-2 receptor-binding domain structures 
            identify distinct epitopes.
  JOURNAL   bioRxiv. 2021 Jan 27. doi: 10.1101/2021.01.27.428466.
  PUBMED    33532775
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2021 Jan 27. doi: 10.1101/2021.01.27.428466."
COMMENT     Sequence Label: pET21b-RBD-JA1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             660..677
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(730..748)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      744..791
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aactttaaga aggagatata catatgaatc tttgtccgtt cggtgaggtt tttaacgcga
       61 caaggttcgc tagtgtatat gcttggaacc gaaagagaat ctccaattgc gtagctgatt
      121 actccgttct ctataacagt gcgtcctttt caacctttaa gtgttacggc gtttctccaa
      181 cgaagctgaa tgatctctgt tttacgaacg tgtatgctga ctctttcgtt atacgggggg
      241 acgaagtgag acagatagca ccaggtcaga ctgggaagat agcggattac aactataagt
      301 tgcccgatga ttttacgggg tgcgtaatcg catggaactc aaacaacctc gactccaaag
      361 taggtggtaa ttataattac ttgtatcgcc tgtttcgaaa gagcaatttg aagccttttg
      421 agcgggatat ttcaaccgaa atttaccaag caggcagtac gccatgtaac ggagtagagg
      481 gatttaattg ctactttcct cttcaatctt atggctttca accaacaaac ggagtggggt
      541 atcaacctta tagagtggta gtattgtcct ttgagctcct ccacgccccg gctacagttt
      601 gtgggcccaa aaagggtgag aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      661 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      721 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      781 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      901 cgcc
//
