LOCUS       sequence-327618-        9799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid for GFP-RPA70.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pQCXIP-GFP-RPA70
FEATURES             Location/Qualifiers
     source          1..9799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        186..565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        566..769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             770..945
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1008..1207
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1408..1824
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1754..1776
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1792..1808
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1861..2164
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2165..2368
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2365..2389
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2507..2509,2510..2512,2513..3223)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2507..2509,2510..2512,2513..3223)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2552..2573)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2813..2832)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3160..3181
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3233..3257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5108..5132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5182..5756
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5350..5367)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5577..5596
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5789..6388
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5789..5808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6285..6305
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6762..7187
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7290..7312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7448..7468)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7453..7782
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7633..7768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7804..7823
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7904..7921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8075..8663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8155..8174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8834..9625,9626..9694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9457..9476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9695..9799)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt ggccattgca
      121 tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc
      181 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      421 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      481 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc aataaaagag
      781 cccacaaccc ctcactcggc gcgccagtct tccgatagac tgcgtcgccc gggtacccgt
      841 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gtctcgctgt tccttgggag
      901 ggtctcctct gagtgattga ctacccacga cgggggtctt tcatttgggg gctcgtccgg
      961 gatttggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca
     1021 acttatctgt gtctgtccga ttgtctagtg tctatgtttg atgttatgcg cctgcgtctg
     1081 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
     1141 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
     1201 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
     1261 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
     1321 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
     1381 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1441 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1501 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1561 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1621 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1681 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1741 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1801 gccctcactc cttctctagg cgccggaatt gaagatctgg gggatcgatc ctctagagtc
     1861 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1921 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1981 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2041 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2101 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2161 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2221 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2281 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2341 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     2401 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     2461 ggaacggtgc attggaacgg acctgcagga attgatccgc ggccgcatgg tgagcaaggg
     2521 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2581 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2641 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2701 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2761 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2821 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2881 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2941 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3001 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3061 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3121 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3181 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaccggat caacaagttt
     3241 gtacaaaaaa gcaggctgcg tcggccagct gagcgagggg gccattgcgg ccatcatgca
     3301 gaagggggat acaaacataa agcccatcct ccaagtcatc aacatccgtc ccattactac
     3361 ggggaatagt ccgccgcgtt atcgactgct catgagtgat ggattgaaca ctctatcctc
     3421 tttcatgttg gcgacacagt tgaaccctct cgtggaggaa gaacaattgt ccagcaactg
     3481 tgtatgccag attcacagat ttattgtgaa cactctgaaa gacggaagga gagtagttat
     3541 cttgatggaa ttagaagttt tgaagtcagc tgaagcagtt ggagtgaaga ttggcaatcc
     3601 agtgccctat aatgaaggac tcgggcagcc gcaagtagct cctccagcgc cagcagccag
     3661 cccagcagca agcagcaggc cccagccgca gaatggaagc tcgggaatgg gttctactgt
     3721 ttctaaggct tatggtgctt caaagacatt tggaaaagct gcaggtccca gcctgtcaca
     3781 cacttctggg ggaacacagt ccaaagtggt gcccattgcc agcctcactc cttaccagtc
     3841 caagtggacc atttgtgctc gtgttaccaa caaaagtcag atccgtacct ggagcaactc
     3901 ccgaggggaa gggaagcttt tctccctaga actggttgac gaaagtggtg aaatccgagc
     3961 tacagctttc aatgagcaag tggacaagtt ctttcctctt attgaagtga acaaggtgta
     4021 ttatttctcg aaaggcaccc tgaagattgc taacaagcag ttcacagctg ttaaaaatga
     4081 ctacgagatg accttcaata acgagacttc cgtcatgccc tgtgaggacg accatcattt
     4141 acctacggtt cagtttgatt tcacggggat tgatgacctc gagaacaagt cgaaagactc
     4201 acttgtagac atcatcggga tctgcaagag ctatgaagac gccactaaaa tcacagtgag
     4261 gtctaacaac agagaagttg ccaagaggaa tatctacttg atggacacat ccgggaaggt
     4321 ggtgactgct acactgtggg gggaagatgc tgataaattt gatggttcta gacagcccgt
     4381 gttggctatc aaaggagccc gagtctctga tttcggtgga cggagcctct ccgtgctgtc
     4441 ttcaagcact atcattgcga atcctgacat cccagaggcc tataagcttc gtggatggtt
     4501 tgacgcagaa ggacaagcct tagatggtgt ttccatctct gatctaaaga gcggcggagt
     4561 cggagggagt aacaccaact ggaaaacctt gtatgaggtc aaatccgaga acctgggcca
     4621 aggcgacaag ccggactact ttagttctgt ggccacagtg gtgtatcttc gcaaagagaa
     4681 ctgcatgtac caagcctgcc cgactcagga ctgcaataag aaagtgattg atcaacagaa
     4741 tggattgtac cgctgtgaga agtgcgacac cgaatttccc aatttcaagt accgcatgat
     4801 cctgtcagta aatattgcag attttcaaga gaatcagtgg gtgacttgtt tccaggagtc
     4861 tgctgaagct atccttggac aaaatgctgc ttatcttggg gaattaaaag acaagaatga
     4921 acaggcattt gaagaagttt tccagaatgc caacttccga tctttcatat tcagagtcag
     4981 ggtcaaagtg gagacctaca acgacgagtc tcgaattaag gccactgtga tggacgtgaa
     5041 gcccgtggac tacagagagt atggccgaag gctggtcatg agcatcagga gaagtgcatt
     5101 gatgtgaacc cagctttctt gtacaaagtg gttgatccgg taggcctcgt acgcttaatt
     5161 aacggatccg gaattccscc ccccctctcc ncycccccgc ccctaacgtt actggccgaa
     5221 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     5281 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     5341 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5401 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5461 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     5521 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     5581 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     5641 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     5701 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
     5761 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc
     5821 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5881 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5941 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6001 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6061 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6121 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6181 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6241 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6301 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6361 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     6421 aaggagcgca cgaccccatg gctccgaccg aagccacccg gggcggcccc gccgaccccg
     6481 cacccgcccc cgaggcccac cgactctagt cgagggctgc agcgctgcag aggccgagtg
     6541 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag
     6601 ccagtgtaga gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc
     6661 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     6721 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     6781 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     6841 agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac
     6901 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     6961 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     7021 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     7081 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     7141 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     7201 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc
     7261 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7381 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc
     7441 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7501 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7561 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7621 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7681 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7741 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact
     7801 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7861 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     7921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9121 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     9181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9421 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9781 aacaaatagg ggttccgcg
//
