LOCUS       sequence-327619-        3408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5053
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(166..754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(246..265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(901..1710)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1624..1643
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1702..1721
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1823..1839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1823..1839
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1844..1863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1844..1862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1880..1979
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             2812..2832
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2844..2943)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2959..2976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2959..2975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2968..2990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3039..3125
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3217..3244
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(join(3403..3408,1..12))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
       61 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      781 gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc atgagcttgc
      841 gccgtcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga
      901 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
      961 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     1021 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     1081 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     1141 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     1201 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     1261 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     1321 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     1381 ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc
     1441 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     1501 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     1561 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac
     1621 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     1681 cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     1741 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     1801 ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata
     1861 ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1921 gcaacaaatt gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtc
     1981 ggcgcgccca cccttgtgta tttttgaagc gaatacttga acgccgatta aaggcagccc
     2041 atctgccgat gatatgtttt cgatagtatc agccaacgct ttcgagtgtg tatgaaactc
     2101 tccgagattg ggatcaaata aggtcgctct tttgccatct gatgatacag aaaagcccaa
     2161 agcgtgggca atttcttggc tgtatatcac cattacccct ctcttatttt ctacaattgc
     2221 atcacataag gagcttagcg cagcttcatc tgtgctcacc tgccgccctg actgcttaca
     2281 cgcaattagg aagggtaggc ctgagttcga cttgtcctct agaacaggtt gaagctttaa
     2341 ccccttcaac tcagcagctt gcaataaact ttctgtgcca gccatcgcgc cacttgatac
     2401 tccggggttt gcggaagctt gctccatcag aaatttgtgc tcagtccgat agaagttatg
     2461 aacagtacga gcatgcacca ccccttcaaa ggaacccaga aacctcattc tttctgccga
     2521 tgcatagggc gtaacatgat ctttcccggc atgaatcatg ttaatccagc tcaatgaaag
     2581 gccaaagcac attgcacgct gactgatgcc atgattattc gtgcaaatat cctgatcgaa
     2641 tctcgccaat gtaattgacg acaattgggt cggaagcttt ttccaccgtc catcactgaa
     2701 ctgtgcagga tcaggcattt gattctcgac cgaagccgaa gacccagctt gaacagtggt
     2761 taggcttggg tgttttccga tgtgtccaga cacgattgtc atggtgaagg gctccttctt
     2821 aaagttaaac aaggcggccg cggagcctgc ttttttgtac aaagttggca ttataaaaaa
     2881 gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2941 ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
     3001 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     3061 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     3121 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
     3181 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
     3241 tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg cgttgcgctc
     3301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgct
//
