LOCUS       sequence-327620-        4716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5588
FEATURES             Location/Qualifiers
     source          1..4716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     235..254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     488..505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(664..691)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(783..869)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     918..940
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     932..949
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     933..949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    965..1064
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             1076..1096
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(3237..3336)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3353..3372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3354..3372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3377..3393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3377..3393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3495..3514)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3506..4315
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3573..3592)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(4462..4716,1..334)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
       61 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      541 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      601 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
      661 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
      721 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
      781 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      841 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
      901 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
      961 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     1021 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt
     1081 taactttaag aaggagccct tcaccatgaa tcctctacga tctattcaac acaacattgc
     1141 aactccccca atcagtggcg gtcagccatt agacgcggtg ggccctcagg cccagcaatc
     1201 ccatcctaaa aggatttcac cttctcaatt gagccaaagc gctcaccagg ctctagaacg
     1261 cctttcagct aatgccgaac accaacgcct tgcatcactg gtacgcaacg ctctgcagga
     1321 tggcacattt caatttcaat ccagtaacca cacgcaagta acctataaag cgtcaatctg
     1381 tctgccagct gacaccgata ccgtgagaac cgaccacttg attaataacg agctgacggt
     1441 tcaggcccga ttaaatgatc aatcggagta cgacatcgtc agcgcacatt tgcatggctc
     1501 ttcgaaagcc atatccttcg acgtacccag ccccccgccc gcacatggtt cagcatcttc
     1561 tgtcttgagt gaacggaccc atctaggtat gagtcgcgtt ctctcacaag atgcagtaga
     1621 cagcagtagc ctggaaactc cgttactgag ctcgccagac cattctcgtc cgccatcaca
     1681 gccaaagccc gtgcatatcg ggtcggtccg cagggactct ggtagccttg tttccgataa
     1741 cccggtagtg caggccctgc tatcgtttgc gcaggccgac caggcatttc caccacaggc
     1801 cgcgagcatt gccggggtcc agctggaaat gcggccacgt cgggatattg agaaagcact
     1861 tgaggaattc aaaggcgcct tcacggtggt gaaggcgcaa ctgatgtccg gtgccaactc
     1921 gtcggagcgt gtagatgagg atgtcaacgc agacatccat atccccttat tgctcaaggc
     1981 catcgagcgg ggggctgcgg catttggtcc aaacgcatca atcggccaga atagcgcgaa
     2041 agcgtttctc gcctcatgtg ctcccaagat cacgtccaat gacgatgtcc tctccgagtt
     2101 catcaaccag aaactcaagg gggacgacga tcttcaggtt cgcctgggcg cacaggaatt
     2161 gttgcatgta gccaccaaga aggaattcca gctcggcggt ctagccggca gcatcggggt
     2221 cagcagcata ctcggctcgg catgggagct tggcgcttct gagctgttga aaaatgccat
     2281 cttcggcaaa aatttctcac cgagccaata tgccctgcaa ttggctggaa tcgattcagt
     2341 gcctcctttg attatcgagt ccatggacac catgtgcgta cttgccatca tcaagggcat
     2401 gaagggtgag gagtggtcca tgagcgatct acttcccaag gcgttgaagg ccggtgctat
     2461 ttcctcggtg gtgtcattcc ccaataatgt tttgcagtat gcaggtttca aatccagagt
     2521 cggcgatctt gcggcaaact cagtgacaac tgaagcggcc atctttggcg ccgcctccgg
     2581 tattccaccc gaggtcaagg aaagtgaaga gctgatgcgt gctggcttat tccagagcat
     2641 gaaggacggc gtgatggctc attcaggcga gggggtggac accaaaaaaa cgattgagcg
     2701 gatgacgcgc catgcgctgg atatcgctcc gggcgaaagc accgctgtca agtccatggg
     2761 gctggcatcg attgtcggga tgattccact gattgccagc aacaaggcaa ccgggctgct
     2821 gtcggaacag gtactgcgta ttttccggag cgccgtcttc aatccaatcg aagccatcgc
     2881 tctgaacgcg ttggcgcttg gcgggcgtgt caacgttccc gggctatttg attccgacaa
     2941 tgccaagcat gcacgcgtgg tacaaaccat ccttgcgcgg gccagccagc acatggaagc
     3001 tggagaccgt gacatttccg cagaggagct acatcaaatg ctggctcccc ggagcgagtt
     3061 cctgcgccat gtgggatctg cgattgtcaa cggcatgaat gccagctttg aggcaattcc
     3121 cgccctggtt cggaagcttg gatatggtga ggctccattg gccgaacgta ttccgtatca
     3181 agacctggct gtgcccgaca cgtcgcggca gcccgcaccc aagggtgggc gcgccgaccc
     3241 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3301 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3361 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3421 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     3481 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     3541 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     3601 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     3661 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3721 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3781 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     3841 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3901 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3961 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     4021 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     4081 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     4141 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     4201 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     4261 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     4321 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     4381 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     4441 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4501 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4561 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4621 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4681 aatcctgtta ccagtggctg ctgccagtgg cgataa
//
