LOCUS       sequence-327626-        2211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30del is conditionally replicating empty vector. Replication of 
            the plasmid is inhibited in presense of IPTG or lactose. pQE30del 
            can be used for construction of any E. coli helper plasmids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
  TITLE     Development of new versatile plasmid-based systems for 
            lambdaRed-mediated Escherichia coli genome engineering.
  JOURNAL   J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001.
  PUBMED    29885886
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001."
COMMENT     Sequence Label: pQE30del
FEATURES             Location/Qualifiers
     source          1..2211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..15,16..21,22..38,39..45)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    21..37
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    53..69
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             88..99
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(207..224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(378..966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(458..477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1137..1928,1929..1997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1760..1779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1998..2102)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2170..2188
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaggagaaat taactatgag aggatcgcat
      121 caccatcacc atcacggatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
      181 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2101 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2161 acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa a
//
