LOCUS       sequence-327627-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Second generation Rosa26 gene targeting vector to induce a 
            Cre-dependent mosaic of cells expressing different membrane 
            localized fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb2-Control-Mosaic (SO273)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..145)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(152..178)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(185..211)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      337..346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    353..386
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    401..522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(412..431)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     466..485
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgccctcgat cacgaaggcg tggccattca cgttgccctc catcttcagc ttgatcttca
       61 tgtcgggctt gattacgccc attgtggtct cctcgccctt gctcaccata agtttggcag
      121 cgtaatctgg aacgtcgtat ggatattcgg ctgcatagtc cgggacgtca tagggatatt
      181 cggccgcata gtcaggaaca tcgtatgggt aggatcctac cttcacgtgg ccattctcct
      241 gtccgttcgc tttggaaggc gacgaggcca cagccgcctc cccaggcctc tccgcggcgg
      301 cttctccctt cgctgcggtc ttggagaaac agcaacccat ggtggcgcgc cgataacttc
      361 gtataatgta tgctatacga agttatacta gtccacgcct taagatacat tgatgagttt
      421 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      481 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      541 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      601 tacaaatgtg gtatggctga ttatgatctg gtgcagcagt ctcgacggta tcgatgcggg
      661 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
      721 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc c
//
