LOCUS       sequence-327628-       14571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CONTROL_transfection of mammalian cells with WT Vcl under a 
            beta-actin promoter, with IRES-GFP allowing identification of 
            transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Vilchez-Larrea, S, Valsecchi W, Fernandez-Villamil S,, Lafon Hughes 
            L
  TITLE     First evidences suggesting a role of a tankyrase-binding motif (TBM)
            of vinculin (VCL) in epithelial cells
  JOURNAL   PeerJ
REFERENCE   2  (bases 1 to 14571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ"
COMMENT     Sequence Label: Tol2-GgVCL/WT
FEATURES             Location/Qualifiers
     source          1..14571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6424..6448
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6450..6466
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6476..6494
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9735..9751)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9788..9806)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(9835..9859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(9862..10436,10437..10439,10440..10448)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10029..10046)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10256..10275
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10455..10475
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10521..10523,10524..10526,10527..11240)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10521..10523,10524..10526,10527..11237)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10566..10587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10827..10846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11174..11195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(11248..11266)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    11271..11405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(11468..11488)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11496..11512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    11528..11662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(12101..12288,12289..12300)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12334..12350)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(12387..12405)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12426..12442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12439..12461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12450..12466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12474..12480,12481..12498,12499..12504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12519..12540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12657..12674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12828..13416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12908..12927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13587..14378,14379..14447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14210..14229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14448..14552)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgaaa attccagttt gaagaaactt
     1141 ttcaagactc accttttcac tgttggtctt aatttaggtt gattattttt gtcaatttaa
     1201 taattacatt gtattgttca tattttattg tcttattgtg gtgtttttac agctttttta
     1261 ttgttctgct ttgttactgt ttgccttgta tgcattgtag cgctttgggt ctgagaaaag
     1321 cgcactataa ataaactgta ttattattat tagggatgga catcacggtg gcgcgatggg
     1381 tggcacaatt acctcacagc aagtaggttg ctgtttagag ccccggctgg atcagttggc
     1441 agttctgtgt ggagtttgca tgttctccct gttcaagtag gtttcctccc ggtgctctgg
     1501 tttcccctca caagtccaaa gacatgatgt ataggtgaat ggggtaagct aaattgacca
     1561 tagtgagtgt gtgtgtgtgt gtgaatgaga gtgtataggt gtttcccagt gttaggttgc
     1621 aactggaagg acatctgctg tgtaaaacat atgcaggata agttggcggt tcattcccct
     1681 atggtgaccc ctggttaatt aaaggactaa gttgaaaatg aatgaatgaa tgaatgatta
     1741 gagataaacc gataccactt ttttacaaac cgatatgagt acaagtataa tagtttctgt
     1801 tactcgacaa tactgagtac cgattctgat acttcttgga tttggtttca atgtaattaa
     1861 tttgaaagaa ggttttattt cttgcctgtc gcaaggatga acacaaaaat ctttaccaga
     1921 aggatatttt aagctaagaa tatacgcacg ttgttgacaa ccctgtatcg attaccgatt
     1981 aaacgtggac atttaaagga taagttatgc ttgcgccatc acaactttac acattacatg
     2041 atcaaagatc atattgaata ttcggtttat tcgtcagaag cctatagatt tgttaaaagt
     2101 gtattttata aacggatggt ttataaagac taagctatta atatatcaga cattcattaa
     2161 tacatcaaga catcgatgtc aggtaggaca acaaatcaca tccatgtaat gaaatcttgt
     2221 tgagtggtat tggtgcgtgg tttcagcatt tcatgtgcaa tacgagtaag aatattttaa
     2281 ccaagtatcg acccaaaacc tgatactggt atcggtgcat cccttattat tattattatc
     2341 aaaaacacac ttgttttatt tatgtgggtg ccaaacaaca aactatgaaa tgcagcatga
     2401 cacgcccaaa aagtgttcct ttgagaacaa cttcataaat atgtttctta tcattatgca
     2461 tgaatggtgt actgggagag ctttggtatc agaattgaga taagagactt tggcattcta
     2521 aaggacaatg acattatcac actgacagtc aacaacactt tattgctctg gaaatcttat
     2581 gtcatggggc aaagctgagg ttcattagga cagtacctta tttagagatg aatgggaagg
     2641 ctgtagtggg ctgctatcag ggaagggtgg tggtcctcgg gggaactatg gactcacaaa
     2701 agcacatctg ctgtaacaag atcccctgtg caatctacaa aagttacaaa gagaagcatg
     2761 gttggttaaa aaatagaatt gactggatat gctgaatgca ttgtttgttg tgtgttacat
     2821 cacattaacc acagattggt gctaaatatt tgcttatctt ataaaaacct gtgtgtttat
     2881 aaaggggggt gtggatggat gcttggcatt ctttgaaaag atgttggggg atggaaggag
     2941 gagaccttat tgatgagtat ccagtttgca gataacaagt ttttagacat tcatactatg
     3001 atgctgttta tgtctgtttt ttctcatttt ttctatacac aaacagctat aaaacattca
     3061 cttttatatc tgatgctgta tattaaattt cgttgtattt attctgtttg ctctgttggg
     3121 tcatagctag ggccagacag aatctgctga cattttttgc tttttctgca gagaattttg
     3181 taaacaatct gtgatttatg cgagattttt tcgggagtat cgtaactaaa aacttactat
     3241 atgaaataaa aaaaaataat acctttttaa cttttaataa atatatacaa tgcaagtcca
     3301 cctagatcca attatttggt cgacaaagca agtctctcac ataatatatc ttctaaaaga
     3361 cagaaaacat tactttcaga ccatattgta atcaaatgaa cactttcata ttagtcaata
     3421 atattacgga aatgtattta aaaactgaat aaacatatat ttacaccttt gtctttattt
     3481 ctagagtgct tttacaatgt agattgtgtc aaagcagctt aacttagagg attagtatag
     3541 taaattgaaa ctgtgtcagt ccatttttta agagtttaag ttcagttcag tttagttcag
     3601 tgtgagttaa tttttactgc tgagagtcca aacactaaag agcaaatcca aatacacaag
     3661 tcaataaata aactcaatga tgggctaaag atctggggaa atctgcagat ttctgtgcgc
     3721 acagattctg tgtgggccta gtcattacct atactcaacg tttactctga gaaacttcca
     3781 tattttagag gtaaaatgct cagaattgta aaaagtcttg tcaaaatact tggaatttgc
     3841 caaaaacaaa ataaaagtca catcacctca ttgtccttgg tatcagcaag cacttttaac
     3901 acaatcagct gtttttgttt ataactccca ctgaaggcca tgtgcattac tcccaagtta
     3961 cacagtaaaa agtcacatgt agtttatcat gatcactgct gctcaagcac agctttatct
     4021 gttattcagt gtggcaatat ctgaaatgtg tgcaaaatat tatttattgt agctgatcac
     4081 caacactttc agtgtttttt ttttctactt ttaatgagaa ttatgttctt aggatttatc
     4141 cttataaacc tctttaaaaa gaaatgttat gtctgcatag acacgacatg aaattatgaa
     4201 cagaaaattt aactatttaa gatcaagact tgacatataa catttctaat gaacattaaa
     4261 cctgaggtag gcccacttgc aaaaatgtaa ctgctatcac tcactttata catcattgtg
     4321 taacttgtac acacaaaaat agtgaagcaa tgtggcatgg aatgcaggtc ccattcattc
     4381 aaagttatta aagcactttc ttaaagtgca ttactactga gctaagacca gctagttgct
     4441 catacaacat actacaagta gtgtcatcct aatttggata aaaaaaatgt ccattttata
     4501 catggaaaag tactcattag atcccccatt gtttgtatta cggtatttcg tgaacacaag
     4561 aggtaaatga cctacagagc tgctgttgtg ttagattgaa aacacaacac aggatcatgg
     4621 aggcatccat cattatgacg cattctgcca cttgtaactc tgcacagact ttttgaaaag
     4681 tttaattgag tcttggcgtt gtcgcagaaa aatacgaaaa attctgtgtt aaaatttgca
     4741 aataaaaatt ccagttttat aggaaatcgc catggccttg ttctttaacc agttaagctt
     4801 ccccttcttt cactctcaag ttgcaagaag caagtgtagc aatgtgcacg cgacagccgg
     4861 gtgtgtgacg ctggaccaat cagagcacag agctccgaaa gtttaccttt tatggctaga
     4921 gccgggtatg tgccgtcata taaaagagcg cgcccagctt ttcagcctca ctttgagctc
     4981 ctccacacgc agctagtgcg gaatatcatc tgcttgtaac ccattctctt aagtcgacaa
     5041 ccccccaaac ccaaggtgag ttggttttac agtttttaag cgtttaatta tagtttctat
     5101 ctattaatgt taagtaaaat gtgtaaatgg ataaatatga tttgatgaag tctttagaca
     5161 gttcataacg gtctctttgt agttatctac taggtttgat ttataagtgg tggttctttt
     5221 gataatctat gttacattta ataaatattg gctgtatctt aacttttttg tagcgttagt
     5281 ggatgtggca ggtgagaatg tcggtaattt aacgtgacca gtcgtaggca cgacattgaa
     5341 cattgaatag gccggtgtga aatgggtgtt aagtcttaat ataaagttgt gttctggcta
     5401 atctctcttt aacagccata aatgtgattt ttaactacag ttttgagggt gctggtgagc
     5461 atgttgcaca cttgatggat agccggcatg ggaagttctt tgtgcaggca gtgctgcagc
     5521 agggtgtgac ctactttagc tagccggcta accagctctc atctgatgta acgtaaaccc
     5581 catcaatcta acatcggctc attcgtgctt tataagatcg aagatcttgt tattagtaac
     5641 gggtattggc atttagtttt aacttggtta attttctttt tgttatgtgt tctatttaaa
     5701 tgtagcctgc agccccctct gtctttaatg aagctgaaga ttaacgttaa cgttagtgaa
     5761 gtgttgtatc cgctatgaaa gatttgaatt ttagaggtgt ggtccacttt aataacggct
     5821 gcggagttct ctggctgcac ttcagccgct gtgactgtcg aagcagagcc catcagtttt
     5881 tcaggatctc ggtgattttt gaggctagac tatgaactga accgactgag agtgaccgtc
     5941 cagaaatgct ttagtgtgaa gtctccgctc acgaggagtc cgtggactga ctgcagatct
     6001 gtgacgcagt aacacccggc ggcagacacc cgttgaaatg cggttgtgta attgatcgca
     6061 ggcgagggtg gaagaggatg tgaaacttca tttgtgtaaa atttagggag tggtcctggt
     6121 gtgatgaatg tcgaaatctg ttccttttta ctgagcccta cgactctggc tgagtgccac
     6181 accgccggca gccgcaaagc ggctcaaacc attgcctttt atggtaataa tgagagaatg
     6241 cagagggact tcctttgtct ggcatatctg aggcgcgcgc tgtcactagc gcccaccagc
     6301 ggtcagactg tagaatgcag agcaaaacag gaagttgact ccagatggtc acatgcttgc
     6361 tgaaacgcct ctccctggca gcagtgcaat tctaacagtt tccctttctt ttacagttca
     6421 gcctcaagtt tgtacaaaaa agcaggcttg taaaacgacg gccagtgagc gcgcgtaata
     6481 cgactcacta tagggcgaat tgggtaccac catgatgccc gtcttccaca cgcgcaccat
     6541 cgagagcatc ttggagcccg tggctcagca gatctcccac ctggtcatca tgcacgagga
     6601 gggggaggta gacggcaagg ccatcccgga cctcaccgcc cccgtgtcgg ccgtgcaggc
     6661 cgctgtcagc aacctggtgc gggttggaaa agaaactgtg cagacaacag aagaccagat
     6721 cttgaaaagg gatatgccac cagcattcat caaagtagag aatgcctgca ccaagctcgt
     6781 tcgagcagcc cagatgctgc aagcagatcc ttattcagta ccagctcgtg actacctaat
     6841 tgatggatca agaggcatcc tttctggaac atcagactta cttctgacat ttgatgaagc
     6901 ggaggtccgt aaaatcatcc gtgtctgcaa aggaatattg gaatatctga ctgtggcaga
     6961 agtagtagag actatggagg atttggtgac atatacaaag aatctagggc caggaatgac
     7021 aaagatggcg aaaatgatcg atgagagaca acaggaatta actcatcagg aacatagggt
     7081 tatgctggtg aactccatga atactgtgaa ggagctattg cctgtactta tttcagctat
     7141 gaagatcttt gtaaccacaa aaaacactaa aagccaggga atagaagagg ccttgaaaaa
     7201 tcgcaatttc acagtagaga aaatgagtgc tgagataaat gaaataatcc gtgtattaca
     7261 actcacttcc tgggatgaag atgcctgggc cagcaaggac actgaagcca tgaaaagagc
     7321 tttagcccta atagattcaa agatgaacca ggcaaaaggc tggctgagag atccaaacgc
     7381 acctccaggg gatgctggtg agcaagcaat aaggcagatc cttgatgaag ctggaaaagc
     7441 aggagaattg tgtgcaggca aagaacgcag ggagattctg ggaacatgca aaactttggg
     7501 ccaaatgact gatcaacttg ctgatctccg agctagagga cagggtgcta caccgatggc
     7561 aatgcagaag gcacagcagg tgtcacaagg cctggatttg ctcactgcaa aagtggagaa
     7621 tgcagcccgg aaattggagg ccatgacaaa ctctaagcag gctatcgcaa agaagattga
     7681 tgctgctcag aactggcttg cggatcccaa cggcggaagt gaaggagaag aacacattcg
     7741 aggaattatg tctgaagcaa ggaaagttgc agaattgtgt gaggagccta aagaaagaga
     7801 tgatatcctt cgctccttgg gggaaatctc tgctctgaca gctaagctgt cagatctgcg
     7861 acgacatggg aaaggcgact ctcctgaggc ccgtgcattg gccaagcaaa tagctacatc
     7921 acttcagaac ttacagtcca aaacaaacag agctgtagca aatactaggc cagttaaagc
     7981 tgctgtccat ttggagggca agattgagca agcccagagg tggatagata atcctacagt
     8041 tgatgatcgg ggagtaggcc aggcagcaat tcggggtttg gttgcagaag gtcgtcgtct
     8101 agccaatgtc atgatgggac cttatcgtca ggacctgctt gccaaatgtg accgtgtaga
     8161 ccagctggct gctcagcttg ctgaccttgc agcaagaggg gaaggagagt ctcctcaggc
     8221 tagggcaatt gctgctcagc ttcaggactc cctgaaggat ctcaaagcac ggatgcaaga
     8281 agcaatgacc caggaggtgt ctgatgtttt cagtgacact acaaccccta ttaagttgtt
     8341 agcagtagca gccactgctc cttctgatac tcccaataga gaagaggtgt ttgaagaaag
     8401 agcagcaaat tttgaaaacc atgctgctag actgggagca acagcagaaa aagcagctgc
     8461 agttggaact gctaataaaa ctactgtgga aggcattcag gcaacagtca aatctgcaag
     8521 ggagcttaca ccacaggtag tatcggctgc tcgaatcctc ctgagaaatc ctggaaatca
     8581 agctgcttat gagcattttg agacaatgaa aaaccaatgg attgataatg tagaaaagat
     8641 gacagggctg gtggatgagg ccatcgatac caagtctctg ttggatgcat cagaagaggc
     8701 tattaagaag gatcttgata aatgcaaagt tgcaatggcc aatatgcaac ctcagatgct
     8761 ggtagctgga gccaccagca ttgctagacg agcgaaccgc atcctgcttg tggcaaaacg
     8821 ggaggttgaa aattcagaag accctaaatt cagggaggct gttaaagcag cttctgatga
     8881 gctgagcaaa accatatcac cgatggtaat ggatgctaaa gctgtagcag gaaatatctc
     8941 tgatcctggt ttgcagaaga gtttcttgga ttctggatac aggattctgg gagctgtggc
     9001 caaagtcaga gaagcctttc agcctcagga gccagacttt ccccctcctc ctcctgacct
     9061 agagcatctc catctgactg atgagcttgc tcctccaaaa ccaccacttc cagaaggtga
     9121 ggttccccca cccagaccac caccccctga agaaaaagat gaggagttcc cagagcagaa
     9181 agcaggagaa gctattaatc agcccatgat gatggctgct aggcagttgc atgacgaggc
     9241 tcggaaatgg tctagcaagg gtaacgacat cattgctgct gctaaacgaa tggcgctgct
     9301 catggctgag atgtcacgcc tggtgcgagg aggcagcgga aacaagcgtg ccctcatcca
     9361 gtgtgcaaaa gatattgcta aggcatcgga tgaagtcact cgattggcca aagaggtggc
     9421 caagcaatgt actgataaac gcattagaac aaacctctta caggtctgtg agcgaatccc
     9481 aaccatcagc acgcagctca aaattctttc cacagtgaaa gctaccatgc tgggcaggac
     9541 taacatcagc gatgaagaat cagaacaggc aactgagatg ttggttcata acgcccagaa
     9601 cctcatgcag tctgtgaagg aaactgtgag agaagctgaa gcagcatcca ttaagataag
     9661 aacagatgcc ggattcactc tgcgctgggt cagaaagacc ccatggtatc agtaggaatt
     9721 cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct
     9781 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagcacccag
     9841 ctttcttgta caaagtggcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     9901 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     9961 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
    10021 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
    10081 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
    10141 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
    10201 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
    10261 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
    10321 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
    10381 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
    10441 ccacaaccat ggctccaaag aagaagcgta aggtaaatcg atcgaattca aggcctctcg
    10501 agcctctaga aggtggcgga atggtgagca agggcgagga gctgttcacc ggggtggtgc
    10561 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
    10621 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
    10681 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
    10741 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
    10801 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
    10861 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
    10921 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
    10981 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
    11041 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
    11101 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
    11161 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
    11221 tggacgagct gtacaagtaa ctgcagccct atagtgagtc gtattacgta gatccagaca
    11281 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
    11341 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
    11401 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
    11461 ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat
    11521 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
    11581 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
    11641 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
    11701 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
    11761 ttatgatcct ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
    11821 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
    11881 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
    11941 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
    12001 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
    12061 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa
    12121 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
    12181 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
    12241 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
    12301 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca
    12361 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    12421 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    12481 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    12541 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    12601 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12661 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12721 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12781 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12841 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12901 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12961 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    13021 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    13081 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13141 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13201 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    13261 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    13321 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    13381 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    13441 gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca
    13501 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    13561 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    13621 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13681 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    13741 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    13801 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    13861 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13921 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13981 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    14041 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    14101 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    14161 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    14221 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    14281 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    14341 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    14401 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    14461 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    14521 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
