LOCUS       sequence-327629-        3895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr16
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(27..615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(107..126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(786..1577,1578..1646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1409..1428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1647..1751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1829..2284)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1966..1987)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2178..2197
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2410..2432
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2424..2441
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2425..2441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2448..2467
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2448..2466
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2497..2506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3484..3502)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3485..3502)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3520..3536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3520..3536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3533..3555)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3544..3560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3568..3574,3575..3592,3593..3598))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3613..3634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3751..3768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
       61 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1081 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1381 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag
     1801 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     1861 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1921 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1981 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2041 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2101 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2161 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2221 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2281 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     2341 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2401 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     2461 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggccgc gggattcctg
     2521 aagtgataga gttcgtcttc ctgggattct ccagctttgg aaagcatcag ataaccctct
     2581 ttgtggtttt cctaaccatc tacattttaa ctctggctgg caacatcatt atagtgacaa
     2641 tcacacacat agaccaccac cttcacactc ccatgtactt ctttctgagc atgttggcaa
     2701 gctcagagac tgtgtacaca ctggtcattg tcccacgaat gctttccagc ctgatttttt
     2761 acaaccttcc catatccttg gcaggctgcg caacccaaat gttctttttt gtcactttgg
     2821 ccaccaacaa ctgctttctg ctcacagcaa tgggttatga tcgttatgtg gctatttgta
     2881 atcctctgag atatacaatc atcatgagca agggaatgtg tgccttgttg gtctgtgggt
     2941 ctttaggcac tggcctggtt atggcagttc ttcatgtgcc agccatgttc catttgccct
     3001 tttgtggcac ggtggtggag cactttttct gtgacatata cccagtaatg aagctttctt
     3061 gtgttgatac caccgtcaat gagataatca attatggtgt aagttcattt gtaattcttg
     3121 tgcccatagg gctgatattt atctcctatg tgctcattgt ctcttccatc cttaaaattg
     3181 tgtccactga aggccagaag aaagcctttg ccacctgtgc ctctcatctc actgtggtca
     3241 ttgtccacta tggctgtgcc tccattgcct acctcaaacc caaatcagaa agttcagtag
     3301 aaaaagacct tcttctctct gtgacctaca ctatcatcac tcccttgctg aaccctgttg
     3361 tctacagcct caggaacaaa gaagtcaaag atgctctatg cagagctgta atcactagtg
     3421 cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag
     3481 tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3541 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3601 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3721 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaac
//
