LOCUS       sequence-327633-        3476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express miR-451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..3476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        68..271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     222..242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    645..726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(737..754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(908..1496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(988..1007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1667..2458,2459..2527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2290..2309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2528..2632)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2665..2746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        join(3240..3476,1..67)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
       61 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      121 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      181 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      241 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctaggg
      301 atcctctagt cagctgacgc gtgctagcgc ggccgcaaga agctctctgc tcagcctgtc
      361 acaacctact gactgccagg gcacttggga atggcaagga aaccgttacc attactgagt
      421 ttagtaatgg taatggttct cttgctatac ccagaaaacg tgccaggaag agaactcagg
      481 accctgaagc agactactgg aagggagact ccaaagcttg tcgacgatat ctccagagga
      541 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      601 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      721 cactgccccg agcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2281 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2641 cccgaaaagt gccacctgac gtcggcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2701 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2761 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2821 cctctacaaa tgtggtatgg ctgattatga tcctctagac tgcagggctg tcggccgcgg
     2881 cggcccccgt ggtgtggagt gcccctcagt gcggcgagag aggtcccggc ctgagataca
     2941 tacttcttta cattccatag cttagcaggt ccatatgggc atataaagaa gtatgtttcc
     3001 caagcaggca gtctgcatgc agttccaaga ccccgggacg ccgccccggt agaagctgac
     3061 cccagatctg ggcccccgcg gcatatgacc ggtgaattct ccaggcgatc tgacggttca
     3121 ctaaacgagc tctgcttata taggcctccc accgtacacg ccacctcgac atactcgagt
     3181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3361 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgccca
//
