LOCUS       sequence-327634-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: GFP-CYLD-STOP
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(671..673,674..676,677..1387)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(704..732)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1327..1347
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    1409..1433
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4313..4337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4369..4386)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4375..4599
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4645..5073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4732..4751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4942..4963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5082..5102)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5087..5416
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5267..5402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5329..5348
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5483..6277
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5537..5556)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6147..6166
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6451..6572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6488..6507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6542..6561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6621..6637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6621..6637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6634..6656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6645..6661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6669..6675,6676..6693,6694..6699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6714..6735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6852..6869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7023..7611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7103..7122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7782..8573,8574..8642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8405..8424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8643..8747)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8822..8841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagacacc atggccagca aaggagaaga acttttcact ggagttgtcc caattcttgt
      721 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      781 tgctacatac ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc
      841 atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga
      901 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg
      961 cactatatct ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg
     1021 tgataccctt gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1081 tctcggacac aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa
     1141 acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt
     1201 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1261 agacaaccat tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga
     1321 ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct
     1381 ctacaaaagc ggttccggac cggatcaaac aagtttgtac aaaaaagttg gcgcgcccac
     1441 catgagttca ggcttatgga gccaagaaaa agtcacttca ccctactggg aagagcggat
     1501 tttttacttg cttcttcaag aatgcagcgt tacagacaaa caaacacaaa agctccttaa
     1561 agtaccgaag ggaagtatag gacagtatat tcaagatcgt tctgtggggc attcaaggat
     1621 tccttctgca aaaggcaaga aaaatcagat tggattaaaa attctagagc aacctcatgc
     1681 agttctcttt gttgatgaaa aggatgttgt agagataaat gaaaagttca cagagttact
     1741 tttggcaatt accaattgtg aggagaggtt cagcctgttt aaaaacagaa acagactaag
     1801 taaaggcctc caaatagacg tgggctgtcc tgtgaaagta cagctgagat ctggggaaga
     1861 aaaatttcct ggagttgtac gcttcagagg acccctgtta gcagagagga cagtctccgg
     1921 aatattcttt ggagttgaat tgctggaaga aggtcgtggt caaggtttca ctgacggggt
     1981 gtaccaaggg aaacagcttt ttcagtgtga tgaagattgt ggcgtgtttg ttgcattgga
     2041 caagctagaa ctcatagaag atgatgacac tgcattggaa agtgattacg caggtcctgg
     2101 ggacacaatg caggtcgaac ttcctccttt ggaaataaac tccagagttt ctttgaaggt
     2161 tggagaaaca atagaatctg gaacagttat attctgtgat gttttgccag gaaaagaaag
     2221 cttaggatat tttgttggtg tggacatgga taaccctatt ggcaactggg atggaagatt
     2281 tgatggagtg cagctttgta gttttgcgtg tgttgaaagt acaattctat tgcacatcaa
     2341 tgatatcatc ccagagagtg tgacgcagga aaggaggcct cccaaacttg cctttatgtc
     2401 aagaggtgtt ggggacaaag gttcatccag tcataataaa ccaaaggcta caggatctac
     2461 ctcagaccct ggaaatagaa acagatctga attattttat accttaaatg ggtcttctgt
     2521 tgactcacaa ccacaatcca aatcaaaaaa tacatggtac attgatgaag ttgcagaaga
     2581 ccctgcaaaa tctcttacag agatatctac agactttgac cgttcttcac caccactcca
     2641 gcctcctcct gtgaactcac tgaccaccga gaacagattc cactctttac cattcagtct
     2701 caccaagatg cccaatacca atggaagtat tggccacagt ccactttctc tgtcagccca
     2761 gtctgtaatg gaagagctaa acactgcacc cgtccaagag agtccaccct tggccatgcc
     2821 tcctgggaac tcacatggtc tagaagtggg ctcattggct gaagttaagg agaaccctcc
     2881 tttctatggg gtaatccgtt ggatcggtca gccaccagga ctgaatgaag tgctcgctgg
     2941 actggaactg gaagatgagt gtgcaggctg tacggatgga accttcagag gcactcggta
     3001 tttcacctgt gccctgaaga aggcgctgtt tgtgaaactg aagagctgca ggcctgactc
     3061 taggtttgca tcattgcagc cggtttccaa tcagattgag cgctgtaact ctttagcatt
     3121 tggaggctac ttaagtgaag tagtagaaga aaatactcca ccaaaaatgg aaaaagaagg
     3181 cttggagata atgattggga agaagaaagg catccagggt cattacaatt cttgttactt
     3241 agactcaacc ttattctgct tatttgcttt tagttctgtt ctggacactg tgttacttag
     3301 acccaaagaa aagaacgatg tagaatatta tagtgaaacc caagagctac tgaggacaga
     3361 aattgttaat cctctgagaa tatatggata tgtgtgtgcc acaaaaatta tgaaactgag
     3421 gaaaatactt gaaaaggtgg aggctgcatc aggatttacc tctgaagaaa aagatcctga
     3481 ggaattcttg aatattctgt ttcatcatat tttaagggta gaacctttgc taaaaataag
     3541 atcagcaggt caaaaggtac aagattgtta cttctatcaa atttttatgg aaaaaaatga
     3601 gaaagttggc gttcccacaa ttcagcagtt gttagaatgg tcttttatca acagtaacct
     3661 gaaatttgca gaggcaccat catgtctgat tattcagatg cctcgatttg gaaaagactt
     3721 taaactattt aaaaaaattt ttccttctct ggaattaaat ataacagatt tacttgaaga
     3781 cactcccaga cagtgccgga tatgtggagg gcttgcaatg tatgagtgta gagaatgcta
     3841 cgacgatccg gacatctcag ctggaaaaat caagcagttt tgtaaaacct gcaacactca
     3901 agtccacctt catccgaaga ggctgaatca taaatataac ccagtgtcac ttcccaaaga
     3961 cttacccgac tgggactgga gacacggctg catcccttgc cagaatatgg agttatttgc
     4021 tgttctctgc atagaaacaa gccactatgt tgcttttgtg aagtatggga aggacgattc
     4081 tgcctggctc ttctttgaca gcatggccga tcgggatggt ggtcagaatg gcttcaacat
     4141 tcctcaagtc accccatgcc cagaagtagg agagtacttg aagatgtctc tggaagacct
     4201 gcattccttg gactccagga gaatccaagg ctgtgcacga agactgcttt gtgatgcata
     4261 tatgtgcatg taccagagtc caacaatgag tttgtacaaa taaggccggc ctacccaact
     4321 ttcttgtaca aagtggtgat aattaattaa gataaacccg ctgatcagcc tcgactgtgc
     4381 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4441 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4501 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4561 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4621 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4681 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4741 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4801 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4861 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4921 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4981 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5041 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5161 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5221 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5281 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5341 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5401 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5461 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5641 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6001 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6241 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6301 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6361 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6421 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6601 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7741 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7801 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7861 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7921 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7981 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8041 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8101 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8161 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8221 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8281 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8341 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8401 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8461 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8521 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8581 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8641 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8701 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8761 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8821 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8881 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8941 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     9001 gatatacgcg tt
//
